编辑AP2/ERF转录因子改善水稻种子落粒性及品质

doi:10.1016/j.jia.2025.02.022

Journal of Integrative Agriculture

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编辑AP2/ERF转录因子改善水稻种子落粒性及品质

修回日期:2025-02-17

Editing of the APETALA2/ethylene responsive factor confers improvements in seed shattering and quality in rice

Saisai Xia¹^{, 2*}, He Liu^2*, Ying Liu², Guangheng Zhang², Deyong Ren²^#, Qian Qian²^#

¹ Rice Research Institute of Shenyang Agricultural University/Key Laboratory of Northern Japonica Rice Genetics and Breeding, Ministry of Education and Liaoning Province/Key Laboratory of Northeast Rice Biology and Breeding, Ministry of Agriculture, Shenyang 110866, China
² State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China

Revised:2025-02-17
About author:#Correspondence Qian Qian, E-mail: qianqian188@hotmail.com; Deyong Ren, rendeyong616@163.com * These authors contributed equally to this work.
Supported by:
This work was supported by the National Natural Science Foundation of China (32372118 and 32188102), the Qian Qian Academician Workstation, and the specific research fund of the innovation platform for academicians of Hainan Province, China (YSPTZX202303), the Nanfan Special Project, Chinese Academy of Agricultural Sciences (ZDXM2315). Chinese Academy of Agricultural Sciences Talent Plan-Outstanding Young Talent, Zhejiang Province’s High-level Talent Special Support Plan-Young Talent.

摘要/Abstract

摘要：

种子的落粒性和品质是维持稳定产量及确保优良食用品质的关键因素。本研究成功鉴定了一个AP2/ERF转录因子—SHAT2，该基因能正向调控种子落粒性和品质。shat2突变体由于离层发育异常，导致成熟期种子难落粒。同时，与野生型相比，shat2突变体的淀粉颗粒排列松散，总淀粉以及直链淀粉含量和蛋白质含量显著降低，而可溶性糖含量显著增加。qRT-PCR 的检测结果显示，大部分与种子落粒性以及品质相关基因在shat2突变体中表达显著下调，表明了SHAT2在水稻种子落粒性和品质中起重要作用。此外，EMSA结果显示SHAT2可以结合GCC-box，暗示SHAT2可能通过调控下游基因的表达来影响种子发育。本研究不仅丰富了遗传资源，还突显了SHAT2在培育具有理想落粒特性和优良稻米品质的水稻品种中的巨大潜力。

. 编辑AP2/ERF转录因子改善水稻种子落粒性及品质[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.02.022.

Saisai Xia, He Liu, Ying Liu, Guangheng Zhang, Deyong Ren, Qian Qian. Editing of the APETALA2/ethylene responsive factor confers improvements in seed shattering and quality in rice[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2025.02.022.

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编辑AP2/ERF转录因子改善水稻种子落粒性及品质

Editing of the APETALA2/ethylene responsive factor confers improvements in seed shattering and quality in rice

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