转录组和代谢组关联分析揭示了梨抗炭疽病的新机制

doi:10.1016/j.jia.2024.12.041

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (10): 3851-3865.DOI: 10.1016/j.jia.2024.12.041

转录组和代谢组关联分析揭示了梨抗炭疽病的新机制

收稿日期:2024-07-28 修回日期:2024-12-31 接受日期:2024-12-11 出版日期:2025-10-20 发布日期:2025-09-24

Integrated transcriptomic and metabolomic analyses reveal a novel mechanism of resistance to Colletotrichum fructicola in pear

Xiaomei Tang^1*, Yue Wang^1*, Yuqing Guo¹, Luoluo Xie¹, Wei Song¹, Ziwen Xiao¹, Ruichang Yin¹, Zhe Ye¹, Xueqiu Sun¹, Wenming Wang², Lun Liu^{1, 3}, Zhenfeng Ye^{1, 3}, Zhenghui Gao^4#, Bing Jia^1#

¹Key Laboratory of Horticultural Crop Quality Biology of Anhui Province, College of Horticulture, Anhui Agricultural University, Hefei 230036, China
²Science and Technology Experiment Station of Shexian County, Huangshan 245200, China
³Jinzhai Modern Agricultural Cooperation Center, Integrated Experimental Station in Dabie Mountains, Anhui Agricultural University, Lu’an 237000, China
⁴Key Laboratory of Horticultural Crop Germplasm Creation and Utilization of Ministry of Agriculture and Rural Areas, Institute of Horticulture, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received:2024-07-28 Revised:2024-12-31 Accepted:2024-12-11 Online:2025-10-20 Published:2025-09-24
About author: *These authors contributed equally to this study. #Correspondence Bing Jia, E-mail: jb1977@ahau.edu.cn; Zhenghui Gao, E-mail: gzh96gao@163.com
Supported by:
This study was supported by the China Agriculture Research System (CARS-28-14), the National Natural Science Foundation of China (32302484), the University Natural Science Research Project of Anhui Province, China (2022AHO50926 and 2022AH040129).

摘要/Abstract

摘要：

由果生炭疽引起的梨炭疽病是一种毁灭性病害，严重影响大多数梨品种的产量和品质。然而，目前尚缺乏有效的防治措施。此外，梨果生炭疽关键抗性调节机制尚不清楚。为了探究梨炭疽病的抗性机制，本研究以梨炭疽病抗性品种“涩梨”和易感品种“翠冠”为材料，对接种果生炭疽和无菌水5天的‘涩梨’和‘翠冠’叶片进行转录组和代谢组关联分析。结果表明，这些差异表达基因和差异积累代谢物主要与代谢和次级代谢合成途径有关，包括α-亚麻酸代谢、苯丙氨酸生物合成代谢、不饱和脂肪酸生物合成、氨基酸及其衍生物的合成等。其中，抗病品种‘涩梨’感染炭疽后差异代谢物主要为不饱和脂肪酸、氨基酸及其衍生物，如亚油酸及其衍生物、月桂酸、N-乙酰-L-谷氨酸和L-脯氨酸的积累显著增加，而易感品种‘翠冠’感染炭疽后氧化型谷胱甘肽和N-乙酰-L-谷氨酸以及原花青素含量显著降低，这些差异代谢物的含量变化可能与‘涩梨’和‘翠冠’炭疽病的抗性差异有关。总之，本研究为梨炭疽病抗性调控提供了新的见解，有助于研发新的梨炭疽病防治策略、以及炭疽病抗性品种的培育。

Abstract:

Pear anthracnose, caused by Colletotrichum fructicola, is a devastating disease that seriously affects most pear varieties, compromising their yield and quality. However, effective control of this pathogen is lacking. Moreover, the critical resistance responses to C. fructicola in pear are unknown. To investigate these resistance mechanisms of pear against C. fructicola, transcriptomic and metabolomic analyses were performed on the anthracnose-resistant variety ‘Seli’ and susceptible variety ‘Cuiguan’ after C. fructicola infection. Differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) were mainly involved in metabolism and secondary metabolite synthetic pathways, including α-linoleic acid metabolism, phenylalanine biosynthesis metabolism, unsaturated fatty acids biosynthesis, and biosynthesis of amino acids and their derivatives. In particular, the accumulation of unsaturated fatty acids (UFAs), amino acids, and their derivatives, such as linoleic acid and its derivatives, lauric acid, N-acetyl-L-glutamic acid, and L-proline, was significantly increased in ‘Seli’ after infection, while the amino acids of oxiglutatione and N-acetyl-L-glutamic acid, as well as the proanthocyanidins, were significantly decreased in ‘Cuiguan’. These findings suggest that these metabolites may contribute to the differential anthracnose resistance between ‘Seli’ and ‘Cuiguan’. Overall, our results provid new insights into the regulation of pear anthracnose resistance, which may assist in developing new control strategies and breeding anthracnose-resistant varieties.

Key words: pear anthracnose , Colletotrichum fructicola , transcriptomic , metabolomic , disease resistance

Xiaomei Tang, Yue Wang, Yuqing Guo, Luoluo Xie, Wei Song, Ziwen Xiao, Ruichang Yin, Zhe Ye, Xueqiu Sun, Wenming Wang, Lun Liu, Zhenfeng Ye, Zhenghui Gao, Bing Jia. 转录组和代谢组关联分析揭示了梨抗炭疽病的新机制[J]. Journal of Integrative Agriculture, 2025, 24(10): 3851-3865.

Xiaomei Tang, Yue Wang, Yuqing Guo, Luoluo Xie, Wei Song, Ziwen Xiao, Ruichang Yin, Zhe Ye, Xueqiu Sun, Wenming Wang, Lun Liu, Zhenfeng Ye, Zhenghui Gao, Bing Jia. Integrated transcriptomic and metabolomic analyses reveal a novel mechanism of resistance to Colletotrichum fructicola in pear[J]. Journal of Integrative Agriculture, 2025, 24(10): 3851-3865.

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转录组和代谢组关联分析揭示了梨抗炭疽病的新机制

Integrated transcriptomic and metabolomic analyses reveal a novel mechanism of resistance to Colletotrichum fructicola in pear

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