miR164a靶向CsNAC1负调控茶树耐寒性

doi:10.1016/j.jia.2024.12.033

摘要/Abstract

摘要：

冷胁迫对茶叶产量的减少和品质的降低有着广泛影响。miR164家族及其靶基因NAC转录因子已被确定为植物应对冷胁迫的关键调节因子之一。然而，人们对miR164和CsNAC在茶树抗寒中的作用知之甚少。本研究发现，csn-miR164a的表达量在冷胁迫下显著降低，且与CsNAC1的表达量呈显著负相关。5 ' RACE和GUS组织定位实验清晰地表明csn-miR164a特异性切割CsNAC1。茶树叶片中csn- miR164a沉默处理后，CsNAC1和CsCBFs的表达水平得以提高，并表现出更强的耐寒性；同时CsNAC1过表达也通过提高AtCBFs的表达水平增强了转基因拟南芥的耐寒性。相反，拟南芥中csn-miR164a异源过表达减少了AtNACs和AtCBFs的表达水平，进而降低了其耐寒性。此外，茶树叶片中CsNAC1的沉默降低了CsCBFs的表达水平，导致其表现出更显著的冷敏感性。综上所述，本研究表明miR164a-CsNAC1模块可能通过CsCBFs依赖途径负调控茶树耐寒性。

Abstract:

Cold stress widely impairs the quality and yield of tea plants. The miR164 family and its target NAC transcription factor have been identified as crucial regulators in response to cold stress. However, the role of miR164 and CsNAC in cold tolerance in tea plants was little understood. In our study, the expression level of csn-miR164a was significantly reduced under cold stress, and was significantly negative correlation with that of CsNAC1. 5’ RACE and GUS histochemical assays clearly showed that CsNAC1 was specifically cleaved by csn-miR164a. The csn-miR164a-silenced tea leaves promoted expression level of CsNAC1 and CsCBFs, and exhibited greater cold tolerance, also overexpression of CsNAC1 enhanced cold tolerance in transgenic Arabidopsis plants by promoting the expression levels of AtCBFs. In contrast, the heterologous overexpression of csn-miR164a in Arabidopsis decreased the expression level of AtNACs and AtCBFs, and thus impaired cold tolerance. Additionally, silencing of CsNAC1-impaired the expression levels of CsCBFs resulted in greater cold sensitivity in tea leaves. Taken together, our present study demonstrated that the miR164a-CsNAC1 module may play a negative role in cold tolerance of tea plants via CsCBF-dependent pathway.

Key words: Tea plants , cold stress , , miRNAs , , NAC transcription factor , , functional verification

. miR164a靶向CsNAC1负调控茶树耐寒性[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.12.033.

Siya Li, Lu Cao, Ziwen Zhou, Yaohua Cheng, Xianchen Zhang, Yeyun Li. The miR164a targets CsNAC1 to negatively regulate cold tolerance of tea plants (Camellia sinensis)[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.12.033.

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