大豆溶血磷脂酸酰基转移酶的全基因家族特征及GmLPAT11在盐胁迫中的功能验证

doi:10.1016/j.jia.2024.12.031

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (9): 3572-3584.DOI: 10.1016/j.jia.2024.12.031

大豆溶血磷脂酸酰基转移酶的全基因家族特征及GmLPAT11在盐胁迫中的功能验证

收稿日期:2024-09-20 修回日期:2024-12-28 接受日期:2024-11-25 出版日期:2026-09-20 发布日期:2026-08-10

Genome-wide characterization of soybean lysophosphatidic acid acyltransferases and functional characterization of the role of GmLPAT11 in salt stress

Zhiyang Wang^*, Peiyan Liu^*, Haitong Sun, Wenying Suo, Ziqian Cheng, Mingliang Yang^#, Qingshan Chen^#, Ying Zhao^#

National Key Laboratory of Smart Farm Technology and Systems/Key Laboratory of Soybean Biology, Ministry of Education/Northeast Agricultural University, Harbin 150030, China

Received:2024-09-20 Revised:2024-12-28 Accepted:2024-11-25 Online:2026-09-20 Published:2026-08-10
About author:Zhiyang Wang, E-mail: wzy13613642168@163.com; Peiyan Liu, E-mail: liupeiyan823@163.com; #Correspondence Mingliang Yang, Tel: +86-451-55191945, E-mail: mlyang@neau.edu.cn; Qingshan Chen, Tel: +86-451-55191259, E-mail: qshchen@126.com; Ying Zhao, E-mail: tianshi198937@126.com * These authors contributed equally to this study.
Supported by:
This study was financially supported by the Natural Science Foundation of Heilongjiang Province, China (TD2022C003 and YQ2022C010), the National Key R&D Program of China (2023ZD040360305), and the National Natural Science Foundation of China (U20A2027, U23A201783, 32272093, and 32272072).

摘要/Abstract

摘要：

溶血磷脂酸酰基转移酶（LPAT）是一种广泛表达的酶，在植物的生长发育和胁迫响应中起着关键作用。然而，关于大豆基因组中的LPAT基因的信息却十分有限。通过全基因组分析，鉴定了15个大豆LPAT家族成员，并分析了保守蛋白基序的存在。根据其系统发育关系，将这些基因分为三个聚类。通过共聚焦显微镜在拟南芥叶肉原生质体上测定了6个GmLPATs的胞内定位。顺式调节元件分析和实时定量聚合酶链反应（qRT-PCR）的结果显示，非生物胁迫（干旱、盐和碱度）和激素处理显著增加了GmLPATs的转录本。GmLPATs在不同的大豆组织中表现出独特的表达模式。我们发现，一个soiLPAT亚型（GmLPAT11）在各个处理下的表达水平较高，这可能表明它在大豆对盐胁迫的响应中起了关键作用。GmLPAT11在大肠杆菌中的表达表明，重组酶具有LPAT活性。我们还发现过表达LPAT降低了转基因大豆活性氧（ROS）的形成，显著提高了抗盐胁迫性。基因关联分析表明，GmLPAT11的变异与幼苗的耐盐性密切相关。在GmLPAT11的CDS区发现了一种可能与耐盐性相关的多态性。这些发现不仅提高了我们对LPAT基因家族的理解，还确定了大豆未来增强耐盐性的潜在候选基因。

Abstract:

Lysophosphatidic acid acyltransferase (LPAT) enzymes are widely expressed in various plant species, and they contribute to growth, development, and stress responses. Currently, information regarding the LPAT gene family in soybeans is limited. In this study, genome-wide analyses identified 15 soybean LPATs, which were then evaluated for their conserved protein motifs. These genes were grouped into three clusters based on their phylogenetic relationships. Confocal microscopy was used to visualize the localization of six GmLPATs within Arabidopsis mesophyll protoplasts. cis-Acting regulatory element analyses and qRT-PCR experiments revealed that these GmLPATs were upregulated in response to hormonal stimulation or exposure to abiotic stressors, including drought, alkaline conditions, and salt stress. The expression patterns of these GmLPATs varied among different soybean tissue types. One member of the solLPAT1 subtype (GmLPAT11) was found to be upregulated in response to a range of treatments, highlighting its role in soybean salt stress responses. GmLPAT11 expression in Escherichia coli confirmed the LPAT activity of this recombinant enzyme, and overexpressing this LPAT reduced reactive oxygen species production in transgenic soybean plants, thereby enhancing their salt stress tolerance. Gene association analyses indicated that GmLPAT11 variants are closely associated with seedling salt tolerance, and a polymorphism in the GmLPAT11 CDS region was potentially associated with salt tolerance. These results provide new insights into the nature of the LPAT gene family in soybeans while also identifying promising candidate genes for future research efforts to enhance the overall salt tolerance of soybean crops.

Key words: soybean (Glycine max (L.) Merr.) , Lysophosphatidic acid acyltransferase , salt stress , expression pattern

Zhiyang Wang, Peiyan Liu, Haitong Sun, Wenying Suo, Ziqian Cheng, Mingliang Yang, Qingshan Chen, Ying Zhao. 大豆溶血磷脂酸酰基转移酶的全基因家族特征及GmLPAT11在盐胁迫中的功能验证[J]. Journal of Integrative Agriculture, 2026, 25(9): 3572-3584.

Zhiyang Wang, Peiyan Liu, Haitong Sun, Wenying Suo, Ziqian Cheng, Mingliang Yang, Qingshan Chen, Ying Zhao. Genome-wide characterization of soybean lysophosphatidic acid acyltransferases and functional characterization of the role of GmLPAT11 in salt stress[J]. Journal of Integrative Agriculture, 2026, 25(9): 3572-3584.

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大豆溶血磷脂酸酰基转移酶的全基因家族特征及GmLPAT11在盐胁迫中的功能验证

Genome-wide characterization of soybean lysophosphatidic acid acyltransferases and functional characterization of the role of GmLPAT11 in salt stress

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