2个参与油菜素内酯合成的细胞色素P450同源基因协同调控油菜株高

doi:10.1016/j.jia.2024.12.016

Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (8): 3153-3168.DOI: 10.1016/j.jia.2024.12.016

2个参与油菜素内酯合成的细胞色素P450同源基因协同调控油菜株高

收稿日期:2024-08-09 修回日期:2024-12-13 接受日期:2024-11-21 出版日期:2026-08-20 发布日期:2026-07-08

Two genes of cytochrome P450 regulate plant height via brassinosteroid biosynthesis in Brassica napus

Qianqian Zheng^1*, Xinhua Wang^1*, Zhenzhen Wang¹, Yi Zhang¹, Hao Wang³, Kangxi Du³, Shaohong Fu², Wanzhuo Gong², Hua Yuan³, Weilan Chen³, Bin Tu³, Jin Yang^2#, Yun Li^2#, Ting Li^1#

¹State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China/Sichuan Agricultural University, Chengdu 611130, China
²Chengdu Research Branch, Chengdu Academy of Agriculture and Forestry Sciences/National Rapeseed Genetic Improvement Center, Chengdu 611130, China
³Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China

Received:2024-08-09 Revised:2024-12-13 Accepted:2024-11-21 Online:2026-08-20 Published:2026-07-08
About author:#Correspondence Ting Li, Tel: +86-28-86293167, E-mail: tinli@sicau.edu.cn; Yun Li, Tel: +86-28-82746572, E-mail: liyunxpzh@163.com; Jin Yang, Tel: +86-28-82746572, E-mail: yjjing@163.com * These authors contributed equally to this study.
Supported by:
This work was supported by the Science and Technology Innovation 2030-Major Project, China (2023ZD04069), the Natural Science Foundation of Sichuan Province, China (23NSFSC0765), the Postdoctoral Fellowship Program of China Postdoctoral Science Foundation (GZB20230498), the Key Research and Development Projects of Chengdu, China (2023-YF09-00024-SN), and the Sichuan Innovation Team Project, China (SCCXTD-2024-3).

摘要/Abstract

摘要：

油菜，作为全球重要的油料作物之一，是食用植物油的重要来源。现有研究表明优化油菜株型是提高油菜单位面积产量的关键因素之一，但其遗传基础和分子调控机制研究尚未明确。为了创制油菜矮杆新种质挖掘控制株高的新基因，我们利用EMS诱变技术对油菜品种“中双11”进行诱变筛选，获得一份矮杆突变体dm1。形态学分析显示发现dm1矮化表型是由于细胞长度显著减少导致的。结合BSA-seq进行定位分析，鉴定到参与dm1株高调控的基因是BnaA10.CYP90A1和BnaC09.CYP90A1。上述2个基因编码细胞色素酶P450，与拟南芥中的AtCPD/AtCYP90A1同源。AtCPD/AtCYP90A1对油菜素内酯（BR）的生物合成至关重要，在本文研究中，我们也发现dm1中具有生物活性油菜素内酯（CS）及其前体6-脱氧CS水平显著降低，并导致下游参与细胞扩张相关基因表达下调。此外，BR水平的降低还通过负反馈机制促进了BR生物合成基因的表达。最后，我们分离获得BnaA10.CYP90A1的单突突变体，发现BnaA10.CYP90A1基因的单一突变导致了植株呈现出半矮化表型，这种表型有望应用于未来的半矮杆育种，以实现产量和机械收获的平衡。综上所述，以上研究表明BnaCYP90A1s参与油菜中油菜素内酯生物合成，并证实它们在调控油菜植株高度中的重要作用，为油菜株型改良提供了新的基因资源和遗传材料。

Abstract:

Rapeseed (Brassica napus) is one of the most important oil crops worldwide and provides a major source of edible vegetable oil. Currently, manipulating plant height with branching effectively balances biomass and yields. However, the genetic mechanisms to control plant height remain largely unknown in rapeseed. To address this gap, we isolated an extremely dwarf mutant (dm1) from ethyl-methanesulfonate (EMS) mutagenesis and revealed that the dwarfism results from a significant reduction in cell length. Bulk segregant analysis (BSA) identified BnaA10.CYP90A1 and BnaC09.CYP90A1 as the causative genes of dm1. Both genes encoded the proteins homologous to the Arabidopsis cytochrome P450 AtCPD/AtCYP90A1, which is crucial for brassinosteroid (BR) biosynthesis. In this regard, we demonstrated reduced levels of bioactive BRs, castasterone (CS), and its precursor 6-deoxoCS in dm1, resulting in down-regulation of various genes involved in cell expansion. The reduced BR levels also caused negative feedback, promoting the expression of BR biosynthetic genes in dm1. Furthermore, we proved that the single mutation of BnaA10.CYP90A1 gene conferred semi-dwarfism, potentially beneficial for producing an ideal type of plant to improve cultivars with a balance of yield and machinery harvest through genetic modifications. Collectively, these findings highlighted the critical role of BnaCYP90A1s in BR biosynthesis and validated their influence on plant height regulation in rapeseed.

Key words: plant height , BR biosynthesis , cytochrome P450 , Brassica napus

Qianqian Zheng, Xinhua Wang, Zhenzhen Wang, Yi Zhang, Hao Wang, Kangxi Du, Shaohong Fu, Wanzhuo Gong, Hua Yuan, Weilan Chen, Bin Tu, Jin Yang, Yun Li, Ting Li. 2个参与油菜素内酯合成的细胞色素P450同源基因协同调控油菜株高[J]. Journal of Integrative Agriculture, 2026, 25(8): 3153-3168.

Qianqian Zheng, Xinhua Wang, Zhenzhen Wang, Yi Zhang, Hao Wang, Kangxi Du, Shaohong Fu, Wanzhuo Gong, Hua Yuan, Weilan Chen, Bin Tu, Jin Yang, Yun Li, Ting Li. Two genes of cytochrome P450 regulate plant height via brassinosteroid biosynthesis in Brassica napus[J]. Journal of Integrative Agriculture, 2026, 25(8): 3153-3168.

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2个参与油菜素内酯合成的细胞色素P450同源基因协同调控油菜株高

Two genes of cytochrome P450 regulate plant height via brassinosteroid biosynthesis in Brassica napus

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