NAC家族基因CmNAC34通过与CmNAC-NOR互作正向调控甜瓜果实成熟

doi:10.1016/j.jia.2024.11.041

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (7): 2601-2618.DOI: 10.1016/j.jia.2024.11.041

NAC家族基因CmNAC34通过与CmNAC-NOR互作正向调控甜瓜果实成熟

收稿日期:2024-04-11 修回日期:2024-11-30 接受日期:2024-10-18 出版日期:2025-07-20 发布日期:2025-06-17

NAC family gene CmNAC34 positively regulates fruit ripening through interaction with CmNAC-NOR in Cucumis melo

Ming Ma^1*, Tingting Hao^1*, Xipeng Ren², Chang Liu¹, Gela A¹, Agula Hasi^1#, Gen Che^1#

¹Key Laboratory of Herbage & Endemic Crop Biology, Ministry of Education/School of Life Sciences, Inner Mongolia University, Hohhot 010070, China
²Institute for Future Farming Systems Organization, Central Queensland University, Rockhampton, QLD 4701, Australia

Received:2024-04-11 Revised:2024-11-30 Accepted:2024-10-18 Online:2025-07-20 Published:2025-06-17
About author:#Correspondence Gen Che, Tel: +86-471-4992209, E-mail: chegen@imu.edu.cn; Agula Hasi, E-mail: hasiagula@imu.edu.cn * These authors contributed equally to this study.
Supported by:
This research was funded by the National Natural Science Foundation of China (32202513), the Applied Technology Research and Development Foundation of Inner Mongolia Autonomous Region, China (2021PT0001), the Natural Science Foundation of Inner Mongolia Autonomous Region, China (2021BS03002), the Inner Mongolia Autonomous Region Universities “Young Science and Technology Talent Support Project”, China (NJYT24067), the Inner Mongolia University High-Level Talent Research Program, China (10000-21311201/056), and the Inner Mongolia Autonomous Region Department of Education First-class Scientific Research Project, China (YLXKZX-ND-030).

摘要/Abstract

摘要：

甜瓜（Cucumis melo）是世界范围内重要的经济园艺作物。NAC（NAM、ATAC和CUC）转录因子在植物生长和果实发育的各个阶段发挥着重要的转录调控作用，但在甜瓜中对其基因功能知之甚少。本研究通过全基因组鉴定和生物信息学分析，在甜瓜基因组中共鉴定出78个含有完整且保守的NAM（no apical meristem）结构域的CmNAC家族基因。转录组数据分析和qRT-PCR结果显示，大多数CmNAC基因在甜瓜的营养器官或生殖器官中特异表达。我们通过遗传转化发现在甜瓜中过表达CmNAC34导致果实早熟，表明该基因对促进果实成熟具有正调控作用。通过酵母双杂交和双分子荧光互补实验，验证了CmNAC34与CmNAC-NOR有直接蛋白互作。CmNAC34和CmNAC-NOR在甜瓜组织中的表达模式相似，亚细胞定位也表明它们均为核蛋白。我们在甜瓜中遗传转化CmNAC-NOR，发现其过表达同样导致果实早熟。酵母单杂交实验和双荧光素酶实验揭示CmNAC34蛋白可以结合两个GLY基因（Glyoxalase）的启动子。这两个GLY基因参与脱落酸信号通路调控果实发育。以上结果揭示了NAC家族转录因子的分子特征、表达谱、功能模式，为CmNAC34调控呼吸跃变期果实成熟的分子机制提供了新的思路。

Abstract:

Melon (Cucumis melo) is an economically important horticultural crop cultivated worldwide. NAC (NAM/ATAC/CUC) transcription factors play crucial roles in the transcriptional regulation of various developmental stages in plant growth and fruit development, but their gene functions in melon remain largely unknown. Here, we identified 78 CmNAC family genes with an integrated and conserved no apical meristem (NAM) domain in the melon genome by performing genome-wide identification and bioinformatics analysis. Transcriptome data analysis and qRT-PCR results showed that most CmNACs are specifically enriched in either the vegetative or reproductive organs of melon. Through genetic transformation, we found that overexpression of CmNAC34 in melons led to early ripening fruits, suggesting its positive role in promoting fruit maturation. Using yeast two-hybrid and bimolecular fluorescence complementation assays, we verified the direct protein interaction between CmNAC34 and CmNAC-NOR. The expression patterns of CmNAC34 and CmNAC-NOR were similar in melon tissues, and subcellular localization revealed their nuclear protein characteristics. We transformed CmNAC-NOR in melon and found that its overexpression resulted in early ripening fruits. Then, the yeast one-hybrid and dual luciferase reporter gene assays showed that the CmNAC34 protein can bind to the promoters of two glyoxalase (GLY) genes, which are involved in the abscisic acid signal pathway and associated with fruit regulation. These findings revealed the molecular characteristics, expression profiles, and functional patterns of the NAC family genes and provide new insights into the molecular mechanism by which CmNAC34 regulates climacteric fruit ripening.

Key words: melon , NAC transcription factors , expression profile , fruit ripening , protein interaction

Ming Ma, Tingting Hao, Xipeng Ren, Chang Liu, Gela A, Agula Hasi, Gen Che. NAC家族基因CmNAC34通过与CmNAC-NOR互作正向调控甜瓜果实成熟[J]. Journal of Integrative Agriculture, 2025, 24(7): 2601-2618.

Ming Ma, Tingting Hao, Xipeng Ren, Chang Liu, Gela A, Agula Hasi, Gen Che. NAC family gene CmNAC34 positively regulates fruit ripening through interaction with CmNAC-NOR in Cucumis melo[J]. Journal of Integrative Agriculture, 2025, 24(7): 2601-2618.

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NAC家族基因CmNAC34通过与CmNAC-NOR互作正向调控甜瓜果实成熟

NAC family gene CmNAC34 positively regulates fruit ripening through interaction with CmNAC-NOR in Cucumis melo

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