‘红早酥’梨Ppbbx24-del突变正调控光诱导花青苷积累

doi:10.1016/j.jia.2024.11.005

‘红早酥’梨Ppbbx24-del突变正调控光诱导花青苷积累

修回日期:2024-11-04

Ppbbx24-del mutant positively regulates light-induced anthocyanin accumulation in the 'Red Zaosu' pear (Pyrus pyrifolia White Pear Group)

Shuran Li^{1, 2*}, Chunqing Ou^1*, Fei Wang¹, Yanjie Zhang¹, Omayma Ismail³, Yasser S.G. Abd Elaziz⁴, Sherif Edris^{5, 6}, He Li^2#, Shuling Jiang^1#

¹Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Germplasm Resources Utilization, Ministry of Agriculture and Rural Affair, Research Institute of Pomology, Chinese Academy of Agricultural Sciences, Xingcheng 125100, China
²College of Horticulture, Shenyang Agricultural University, Shenyang 110866, China
³Horticultural Crops Technology Department, National Research Centre, Cairo, 12622, Egypt
⁴Fruit Breeding Department, Horticulture Research Institute-Agriculture Research Centre, Orman, Giza, 12619, Egypt
⁵Department of Biological Sciences, King Abdulaziz University, Jeddah, 21589, Saudi Arabia
⁶Department of Genetics, Ain Shams University, Cairo, 11241, Egypt

Revised:2024-11-04
About author:Shuran Li, +86-15935458172, E-mail：suera142@163.com; Chunqing Ou, +86-15042929049, E-mail：ouchunqing@caas.cn #Correspondence Shuling Jiang, +86-13998900156, E-mail：jshling@163.com; He Li, +186-13238805558，E-mail: lihe@syau.edu.cn *These authors contributed equally to this study.
Supported by:
We thank the National Natural Science Foundation of China (32072531), the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2016-RIP), the National Key Research and Development Program of China (2021YFE0190700), and the Science, Technology & Innovation Funding Authority (STDF) of Egypt (43093) for funding this work.

摘要/Abstract

摘要：

红梨因外观艳丽、营养丰富深受消费者喜爱，而花青苷是红梨果皮的主要色素。许多具有红色果皮的梨品种起源于芽变，然而这种现象背后的遗传机制尚不清楚。我们前期自‘早酥’梨红色芽变‘红早酥’梨中鉴定出一个发生移码突变的PpBBX24基因，命名为Ppbbx24-del，该基因与‘红早酥’梨花青苷的积累密切相关。在此，我们分析了突变基因在红梨着色中的作用及其作用机制。结果表明，光照促进了‘红早酥’梨果皮着色，Ppbbx24-del对光诱导花青苷的生物合成有促进作用，这与正常的PpBBX24相反。瞬时转化和稳定转化实验证实Ppbbx24-del能促进梨果皮、愈伤组织和烟草花青苷的积累。亚细胞定位结果表明，PpBBX24与Ppbbx24-del蛋白的亚细胞定位不同，PpBBX24定位于细胞核；而由于NLS和VP结构域的缺失，Ppbbx24-del为核质共定位。酵母双杂交、Pull-down和BiFC试验结果表明PpBBX24可以与PpHY5互作，但Ppbbx24-del不能；酵母单杂交、EMSA和双荧光素酶表达活性分析试验结果表明PpHY5、PpBBX24与Ppbbx24-del均可与PpMYB10、PpCHS和PpCHI基因启动子上的G-box元件结合，PpHY5和Ppbbx24-del单独存在时，均对下游基因的表达起正调控作用，二者同时存在时，对下游基因的激活呈累加效应；而PpBBX24单独存在时，对下游基因的表达没有显著影响，与PpHY5同时存在时，抑制了PpHY5对下游基因的激活作用。本研究系统证明了突变的Ppbbx24-del基因由花青苷积累负调控因子突变成了正调控因子，并解析了其作用机制，丰富了植物花青素生物合成调控网络，为利用该基因创制红梨资源奠定了理论基础。

Abstract:

Red fruit peel is one of the most valuable economic traits in pear and is mainly determined by anthocyanins. Many pear cultivars with a red peel originated from bud sports; however, little is known about the genetic mechanisms underlying this trait. We have previously identified a mutant PpBBX24 containing a 14-nucleotide deletion in the coding region (Ppbbx24-del) as the only known variant associated with the red coloration of the mutant 'Red Zaosu' pear (Pyrus pyrifolia White Pear Group). Herein, we analyzed the role of the mutant gene in red coloration and its mechanism of action. The results showed that light promoted red peel coloration in 'Red Zaosu' pear, and Ppbbx24-del had positive effects on light-induced anthocyanin biosynthesis, while normal PpBBX24 had the opposite effects. Transient and stable transformation experiments confirmed that Ppbbx24-del could promote anthocyanin accumulation in pear fruit peels, calli, and tobacco flowers. Due to the loss of NLS and VP domains, Ppbbx24-del co-localized in the nucleus and cytoplasm, whereas PpBBX24 localized only in the nucleus. Real-time PCR and transcriptome analyses indicated that PpMYB10 and PpHY5 is highly expressed in 'Red Zaosu' pear. In yeast one-hybrid and dual luciferase assays, Ppbbx24-del and PpHY5 independently promoted the expression of PpCHS, PpCHI, and PpMYB10 by binding to their promoters; however, PpBBX24 did not affect the expression of these genes. Additionally, we found that Ppbbx24-del and PpHY5 had additive effects on the expression of PpCHS, PpCHI, and PpMYB10, as they promote the expression of anthocyanin synthesis genes separately. The co-expression of PpBBX24 and PpHY5 inhibited the activation of downstream genes by PpHY5, and this was attributed to the interaction between the two loci. In conclusion, our results clarify the molecular mechanism by which mutant Ppbbx24-del and PpBBX24 exert opposite effects in the regulation of anthocyanin accumulation in pear. These findings lay an important theoretical foundation for the use of Ppbbx24-del to create red pear cultivars.

Key words: pear , anthocyanin , Ppbbx24-del , gene mutation , transcriptional regulation

. ‘红早酥’梨Ppbbx24-del突变正调控光诱导花青苷积累[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.11.005.

Shuran Li, Chunqing Ou, Fei Wang, Yanjie Zhang, Omayma Ismail, Yasser S.G. Abd Elaziz, Sherif Edris, He Li, Shuling Jiang. Ppbbx24-del mutant positively regulates light-induced anthocyanin accumulation in the 'Red Zaosu' pear (Pyrus pyrifolia White Pear Group)[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.11.005.

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