Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (4): 1606-1609.DOI: 10.1016/j.jia.2024.09.034

• • 上一篇    

基于甜菜碱生物合成代谢工程创制紫叶花生种质资源

  

  • 收稿日期:2024-07-26 接受日期:2024-09-11 出版日期:2025-04-20 发布日期:2025-03-18

Creation of purple leaf peanut germplasm through metabolic engineering of the betalain biosynthesis pathway

Dongxin Huai1, Jie Wu1, Xiaomeng Xue1, Hao Liu2, Nian Liu1, Li Huang1, Liying Yan1, Yuning Chen1, Xin Wang1, Qianqian Wang1, Yanping Kang1, Zhihui Wang1, Yanbin Hong2, Huifang Jiang1, Boshou Liao1#, Yong Lei1#   

  1. 1 Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture and Rural Affairs/Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, China

    2 Guangdong Provincial Key Laboratory of Crop Genetic Improvement/South China Peanut Sub-Center, National Center of Oilseed Crops Improvement/Crops Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China

  • Received:2024-07-26 Accepted:2024-09-11 Online:2025-04-20 Published:2025-03-18
  • About author:#Correspondence Yong Lei, Tel: +86-27-86812725, E-mail: leiyong@caas.cn; Boshou Liao, Tel: +86-27-86712292, E-mail: lboshou@hotmail.com
  • Supported by:
    This work was supported by the Guangdong Provincial Key Research and Development Program - Modern Seed Industry, China (2022B0202060004), the Knowledge Innovation Program of Wuhan-Basic Research, China (2022020801010291), the Project of the Development for High-quality Seed Industry of Hubei Province, China (HBZY2023B003), and the Innovation Program of the Chinese Academy of Agricultural Sciences (2023-2060299-089-031).

摘要:

在乡村旅游蓬勃发展之际,农田作画已成为吸引游客观光和提升农民收入的关键策略。然而,在我国重要的油料作物——花生中,缺少可供花生田作画使用的紫叶品种。因此,开发紫叶花生品种势在必行。甜菜碱是一种源自氨基酸酪氨酸的植物天然色素,呈现红紫色,并且具备抗氧化功能对人体健康有益。甜菜碱的生物合成途径相对简单,仅通过三个酶反应便可将酪氨酸转化为甜菜碱。RUBY基因是由编码甜菜碱合成所需的P450 加氧酶(CYP76AD1)、二羟基苯丙氨酸双加氧酶(DODA)和糖基转移酶的三个基因组成的人工开放阅读框,可利用酪氨酸合成甜菜碱。本研究采用农杆菌介导的转化方式,在 35S 启动子的驱动下,在花生中异源表达RUBY 基因。在转基因植株的愈伤组织、再生芽以及根、叶、茎、花、豆荚和种子中均观察到了紫色组织存在。与野生型植株相比,紫色叶片中转基因植株含有显著更高量级的甜菜碱。综上所述,我们成功创制出了具备观赏植物潜力的带有紫色叶片特征的花生新种质,为培育紫叶花生品种提供了理论和材料基础。此外,RUBY在花生转化过程中展现出了作为可见报告基因的潜力,可用于高效筛选转化植株。