Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (1): 403-407.DOI: 10.1016/j.jia.2024.09.025

• • 上一篇    

中国家禽分离获得同时存在mcr-1.1和mcr-8.1且产ESBLs的ST15型肺炎克雷伯菌

  

  • 收稿日期:2024-03-10 接受日期:2024-07-01 出版日期:2025-01-20 发布日期:2025-01-07

Emerging patterns of antimicrobial resistance: Concurrent presence of mcr-1.1 and mcr-8.1 in a ST15 Klebsiella pneumoniae isolated from poultry in China

Dong’an Cui1, Panpan Liu1, Ling Wang1, Jiongjie He1, Yuzhang Yan1, 2, Mengke Ru1, 2, Baocheng Hao1, Yan Sun3#, Shengyi Wang1#   

  1. 1 Key Laboratory of New Animal Drug Project, Gansu Province/Key Laboratory of Veterinary Pharmaceutical Development, Ministry of Agriculture and Rural Affairs, Lanzhou Institute of Husbandry and Pharmaceutical Sciences, Chinese Academy of Agricultural Sciences, Lanzhou 730050, China

    2 College of Animal Science and Technology, Guangxi University, Nanning 530005, China

    3 China Institute of Veterinary Drug Control, Beijing 100081, China 

  • Received:2024-03-10 Accepted:2024-07-01 Online:2025-01-20 Published:2025-01-07
  • About author:Dong'an Cui, E-mail: cuidongan@caas.cn; #Correspondence Yan Sun, Tel: +86-10-62103799, E-mail: sunyan@caas.cn; Shengyi Wang, Tel: +86-931-2112161, E-mail: wangshengyi@caas.cn
  • Supported by:
    This work was funded by the National Key R&D Program of China (2023YFD1800800), the Basic Scientific Research Business Expenses of Central Public Welfare Scientific Research Institutes, China (Y2022YJ14), the Technology Innovation Project of Hubei Province, China (2022BEC033) and the Innovation Program of Chinese Academy of Agricultural Sciences-Veterinary Natural Medicine and Antibiotic Replacement, China (25-LZIHPS-03).   

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摘要：

多粘菌素是治疗耐碳青霉烯肺炎克雷伯菌感染的最后一道防线，MCR的可移动性传播将对肺炎克雷伯菌感染的治疗造成巨大威胁。在对宁夏某地方保种品种蛋鸡病原菌的耐药性检测中发现一株多粘菌素耐药且产超广谱β-内酰胺酶（ESBLs）的多药耐药肺炎克雷伯菌，命名为K. pn NXQY01。为探究K. pn NXQY01β-内酰胺类抗生素与多粘菌素耐药性传播风险，对K. pn NXQY01进行全基因组测序与生物信息学分析，探究MCR耐药基因在质粒中的水平传播机制，并通过质粒结合转移验证其水平转移。全基因组测序分析显示，K. pn NXQY01属于ST15型，携带6个质粒，pNXQY01-1（227,409 bp）、pNXQY01-2（110,028 bp）、pNXQY01-3（71,848 bp）、pNXQY01-4（71,087 bp）、pNXQY01-5（66,252 bp）和pNXQY01-7（8,159 bp），质粒共携带了K. pn NXQY01约90%的耐药基因。更重要的是，质粒同时携带了mcr-1.1, mcr-8.1, blaCTX-M-55, blaCTX-M-65耐药基因，具有极大的潜在水平传播风险。质粒序列分析显示，pNXQY01-2可能由质粒pKP4和pPK45_NDM1重组形成。pNXQY01-2携带mcr-8.1，并可能同时携带了编码AmpC β-内酰胺酶的ampC1和ampC2基因。mcr-8.1侧翼序列分析表明其与IS903B元件形成的复合转座子有关。pNXQY01-5与pHNSHP45具有高度相似性，携带mcr-1.1和blaCTX-M-55。pNXQY01-5中耐药基因mcr-1.1和blaCTX-M-55可能是通过外源DNA序列插入获得，与插入序列ISApl1和ISEc9有关。oriTfinder分析显示，pNXQY01-2、pNXQY01-3和pNXQY01-5具有完整的结合转移模块，显示水平转移风险。结合转移试验表明，mcr-1.1、mcr-8.1、blaCTX-M-55和blaCTX-M-65可能通过pNXQY01-2、pNXQY01-3和pNXQY01-5发生了水平转移，同时接合子对β-内酰胺类抗生素、多粘菌素B和庆大霉素的药物敏感性均升高至与K. pn NXQY01相当。本研究首次报告了mcr-8.1与mcr-1.1同时存在于产ESBLs的多药耐药肺炎克雷伯菌分离株的质粒，标志着质粒介导的新抗生素耐药性特征。