植物丝状蛋白 7 (FPP7) 通过增强脱落酸敏感性以及扰乱钠离子和活性氧稳态来负向调节水稻耐盐性

doi:10.1016/j.jia.2024.08.029

摘要/Abstract

摘要：

植物丝状蛋白是一种中间丝蛋白，在植物的发育和生长过程中发挥着重要作用。然而，目前尚未对植物FPP 家族进行系统鉴定和表征。本研究通过分析两种双子叶植物和四种单子叶植物的基因组，发现了 59 个 FPP 候选基因。系统进化分析和共线性图谱显示，FPP基因在单子叶植物中进化相对保守。其中，水稻 8 个 OsFPP 成员均会被非生物胁迫诱导或抑制。遗传学证据表明，过表达OsFPP7水稻植株在芽期受到盐胁迫时，ABA敏感性增加、Na⁺/K⁺和活性氧平衡被破坏。而敲除 OsFPP7 可通过减弱植株对 ABA 的敏感性以及保护抗氧化系统和钠离子转运系统，从而提高水稻耐盐性。本研究结果进一步阐明了细胞骨架、FPP调控盐胁迫功能以及植物抗逆机制之间的关联。

Abstract:

Filament-like plant proteins are intermediate filament proteins that play a major part in the development and growth of plants. However, no systematic identification and characterization have been conducted on the FPP family in plants. Fifty nine FPP candidates were found in this work by analyzing the genomes of two dicots and four monocots. Phylogenetic analysis and multicollinearity mapping showed the relatively conserved evolution of FPP genes in monocots. Herein, eight OsFPPs were characterized and found to be induced or repressed by abiotic stresses. Additional genetic evidence shows that OsFPP7-overexpressing rice exhibited increased sensitivity to abscisic acid during the germination stage, disrupted Na⁺/K⁺ homeostasis, and disrupted the balance of reactive oxygen species during the seedling stage when exposed to salt stress. Conversely, knockout of osfpp7 alleviated ABA sensitivity, safeguarded the antioxidant system and sodium ion transport system, and thus enhanced rice salt tolerance. The cytoskeleton, FPPs’ function in controlling salt stress and plant stress tolerance mechanisms are all further elucidated by our findings.

Key words: OsFPP7 , abscisic acid , , Na⁺ homeostasis , , ROS homeostasis , , rice (Oryza sativa L.)

. 植物丝状蛋白 7 (FPP7) 通过增强脱落酸敏感性以及扰乱钠离子和活性氧稳态来负向调节水稻耐盐性[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.08.029.

Fan Fan, Jin Chen, Lingyue Yan, Wenjie Hu, Xue Liu, Jia Zeng, Ling Liu, Ting Liu, Nenghui Ye, Dingyang Yuan, Meijuan Duan. Filament-like plant protein 7 (FPP7) negatively regulates rice salt tolerance by enhancing abscisic acid sensitivity and disturbing sodium and ROS homeostasis[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.08.029.

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