Journal of Integrative Agriculture

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大白菜生物钟节律影响了糖代谢相关基因的昼夜表达模式

  


  • 修回日期:2024-08-29

Circadian clock shapes diurnal gene expression patterns linked to glucose metabolic processes in Chinese cabbage

Shan Wang, Kailin Shi, Yufan Xiao, Wei Ma, Yiguo Hong, Daling Feng#, Jianjun Zhao#   

  1. State Key Laboratory of North China Crop Improvement and Regulation, Key Laboratory of Vegetable Germplasm Innovation and Utilization of Hebei, Collaborative Innovation Center of Vegetable Industry in Hebei, College of Horticulture, Hebei Agricultural University, Baoding, 071000, China
  • Revised:2024-08-29
  • Supported by:

    This work was supported by S&T Program of Hebei (Grant No. 236Z2903G) and the Innovative Research Group Project of Hebei Natural Science Foundation (Grant No. C2020204111). We are grateful to Hebei International Joint Research Center of Vegetable Functional Genomics, and International Joint R&D Center of Hebei Province in Modern Agricultural Biotechnology for supporting this work. 

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摘要：

生物钟作为植物体内的内源计时系统，通过调控基因表达的节律性以及各种生理代谢过程，从而提高植物对环境变化的适应性和竞争力，以达到最优化生长。大白菜（Brassica rapa ssp.pekinensis）作为十字花科重要的蔬菜作物，具有重要的经济价值。因此，解析生物钟对昼夜节律基因的影响，对提高大白菜的农艺性状和产量具有重要的指导意义。本研究以2份短周期自交系（“SPcc-1”和“SPcc-2”）和2份长周期自交系（“LPcc-1”和“LPcc-2”）为试材，在20ºC持续光照条件下取样进行时序转录组测序分析，发现大白菜32.7~50.5%的基因表达受生物钟调控，且节律基因的表达峰在短周期材料中出现较早，在长周期材料中出现较晚。大约250个可变剪切事件呈昼夜节律变化，其中内含子保留为主要的可变剪切类型。节律剪切基因包括生物钟核心基因：LATE ELONGATED HYPOCOTYL（BrLHY）、REVEILLE 2（BrRVE2）和EARLY FLOWERING 3（BrELF3）。经转录水平及转录后水平分析，生物钟广泛影响着光合作用、卡尔文循环和三羧酸循环等糖代谢相关基因的周期性表达。本研究明确了生物钟参与的主要代谢途径，有助于利用生物钟调控大白菜的生长发育。

Abstract:

The plant circadian clock temporally drives gene expression through the day and coordinates various physiological process with diurnal environmental changes. It is essential to confer plant fitness and competitive advantage to survive and thrive under natural condition by circadian control of gene transcription. Chinese cabbage (Brassica rapa ssp. pekinensis) is an economically important vegetable crop worldwide; however, there is little information concerning its circadian clock system. Here we uncovered that gene expression patterns were affected by circadian oscillators at both transcriptional and post-transcriptional levels in Chinese cabbage. Time-course RNA-seq analyses were conducted on two short-period lines (SPcc-1 and SPcc-2) and two long-period lines (LPcc-1 and LPcc-2) under constant light. We showed that 32.7-50.5% of the genes were regulated by the circadian oscillator and the expression peak of cycling genes appeared even earlier in short-period lines compared to long-period lines. In addition, approximately 250 splicing events showed circadian regulation, of which intron retention (IR) accounted for a large proportion. Rhythmically spliced genes included the clock genes LATE ELONGATED HYOCOTYL (BrLHY), REVEILLE 2 (BrRVE2) and EARLY FLOWERING 3 (BrELF3). We also found that the circadian oscillator could notably influence the diurnal expression patterns of genes that are associated with glucose metabolism via photosynthesis, Calvin cycle and tricarboxylic acid (TCA) cycle at both transcriptional and post-transcriptional levels. Taken together, our results demonstrate that circadian-regulated physiological processes contribute to Chinese cabbage growth and development.

Key words: circadian clock , transcriptomek ,  , alternative splicingk ,  , glucose metabolismsk ,  , Chinese cabbage