Journal of Integrative Agriculture ›› 2026, Vol. 25 ›› Issue (8): 3379-3386.DOI: 10.1016/j.jia.2024.08.025

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母羊铬补充改善子代LPS应激羔羊抗氧化、免疫功能及肠道形态

  

  • 收稿日期:2024-06-12 修回日期:2024-08-27 接受日期:2024-08-21 出版日期:2026-08-20 发布日期:2026-07-08

Maternal chromium supplementation improves oxidation resistance, immunity, and intestinal morphology of goat kids injected with lipopolysaccharide

Yujuan Li1, 2, Chengcheng Dai1, 2, Fang Xiao1, 2, Jerry W Spears3, Yanhua Gao1, 2, Fei Jiang1, 2, Haitao Shi1, 2, Yanling Huang1, 2#   

  1. 1 Key Laboratory of Animal Science of State Ethnic Affairs Commission, Southwest Minzu University, Chengdu 610041, China

    2 Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Reservation and Utilization, Southwest Minzu University, Chengdu 610041, China

    3 Department of Animal Science, North Carolina State University, Raleigh, NC 27695-7621, USA

  • Received:2024-06-12 Revised:2024-08-27 Accepted:2024-08-21 Online:2026-08-20 Published:2026-07-08
  • About author:Yujuan Li, E-mail: hbhsyj@163.com; #Correspondence Yanling Huang, Tel: +86-28-85522310, E-mail: swunylh@163.com
  • Supported by:
    This work was supported by the Sichuan Provincial Key R&D Project, China (22ZDYF0194), the Innovation Team Development Funds for Sichuan Beef, China (SCCXTD-2024-13), the Double World-Class Project of Southwest Minzu University, China (XM2023010), the Scientific and Technological Innovation Team Qinghai-Tibetan Plateau for Research in Southwest Minzu University, China (2024CXTD17).

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摘要：

本试验研究了母羊铬补充对子代脂多糖（lipopolysaccharide, LPS）应激羔羊抗氧化、免疫功能及肠道形态的影响。选取40只妊娠90天（90±3.53天）的乐至黑山羊，按体重（52.36±8.24 kg）随机分为4个处理，每组10个重复，每个重复1只母羊，单栏单饲。对照组饲喂基础日粮（实测铬含量为0.29 mg kg-1），铬补充组在饲喂基础日粮的基础上，每头母羊每日以丙酸铬（Cr propionate, CrPro）的形式补充0.4、1.2或2.0 mg的铬，直至子代羔羊21日龄。羔羊出生当日，采集母羊初乳和脐带样品，测定初乳中免疫球蛋白含量及初乳和脐带中铬含量。羔羊哺乳至21日龄，每重复选取一只雄性羔羊，LPS注射2 h后采血、屠宰，测定羔羊血清及肝脏抗氧化指标、血清免疫球蛋白含量、肝脏铬含量及十二指肠、空肠、回肠肠道形态。结果表明：1）母羊铬补充对子代羔羊的初生重、窝重及21日龄体重、窝重无影响（P>0.05）。2）母羊初乳、脐带及子代羔羊肝脏铬含量随母羊铬补充量的提高线性增加（P<0.05）。3）随母羊铬补充量提高，子代羔羊血清总超氧化物歧化酶（Total Superoxide dismutase, T-SOD）活性呈二次曲线升高、丙二醛（Malondialdehyde, MDA）含量线性降低，谷胱甘肽过氧化物酶（Glutathione peroxidase, GSH-Px）活性线性升高（P<0.05）。4）随母羊铬补充量的增加，初乳中免疫球蛋白A（Immunoglobulin A, IgA）、免疫球蛋白G（Immunoglobulin G, IgG）和免疫球蛋白M（Immunoglobulin M, IgM）含量线性增加；子代羔羊血清IgG和IgM含量随母羊铬补充量的增加呈二次曲线增加，均在铬补充量为每头每日0.4 mg时达到最高（P<0.05）。5）随母羊铬补充量的增加，子代羔羊十二指肠绒毛高度（Villus height, VH）和绒毛高度/隐窝深度（Villus height to crypt depth ratio, V/C）呈二次曲线升高，且均在铬补充量为每头每日1.2 mg时达到最高；回肠隐窝深度（Crypt depth, CD）线性降低（P<0.05）。本试验范围内，每头母羊每日补充0.4-2.0 mg（实测值0.47-2.41 mg）来源于丙酸铬的铬可改善子代LPS应激羔羊抗氧化、免疫功能及肠道形态。本研究首次发现铬补充可提高母羊初乳免疫球蛋白含量及初乳和脐带铬沉积，并改善了子代LPS应激羔羊的抗氧化、免疫功能及肠道形态，研究结果为铬在妊娠动物中的应用提供了理论依据。

Abstract:

This study evaluated the effects of maternal chromium (Cr), supplemented with Cr propionate (CrPro) during perinatal period of does, on antioxidant capacity, immune response, and intestinal morphology of goat kids injected with lipopolysaccharide (LPS).  Forty Lezhi black does at 90 d of pregnancy were divided into 4 groups based on body weight (52.36±8.24) kg with 10 replicate pens for each treatment and one doe for each replicate pen.  Treatments consisted of 0, 0.4, 1.2, or 2.0 mg of supplemental Cr per head per day (per head d–1) from CrPro.  After birth, the goat kids were allowed to suckle until 21 d.  At 21-d-old, one male goat kid per replicate was sacrificed 2 h after LPS injection.  Individual weight and litter weight of goat kids at birth and 21 d were not affected by maternal Cr supplementation.  Chromium concentrations in umbilical cord, colostrum and liver of goat kids were linearly increased by maternal Cr supplementation (P<0.05).  Maternal Cr supplementation quadratically increased serum total superoxide dismutase (T-SOD) activity of goat kids (P<0.05).  Goat kids from maternal Cr supplementation groups had higher serum T-SOD activity than those from the control group (P<0.05).  Serum malondialdehyde (MDA) concentration of goat kids was linearly decreased, whereas serum glutathione peroxidase (GSH-Px) activity was linearly increased as maternal Cr increased (P<0.05).  Maternal Cr supplementation linearly increased colostrum immunoglobulin A (IgA), immunoglobulin G (IgG) and immunoglobulin M (IgM) concentrations (P<0.05), and quadratically increased serum IgG and IgM concentrations of goat kids with the greatest response detected at 0.4 mg
per head d–1 maternal Cr supplementation group (P<0.05).  The villus height (VH) and the ratio of VH to crypt depth (CD) (V/C) in the duodenum of goat kids increased quadratically as maternal Cr increased, and both reached the highest values in the 1.2 mg per head d–1 maternal Cr supplementation group.  Maternal Cr supplementation linearly decreased ileal CD of goat kids.  The results indicate that 0.4–2.0 mg (0.47–2.41 mg by analysis) per head d–1 maternal Cr supplementation from CrPro could improve antioxidant capacity, immune response, and small intestinal morphology of goat kids injected with LPS.  

Key words: maternal chromium supplementation , goat kids ,  antioxidant capacity ,  immune response ,  intestinal morphology