利用小麦-粗山羊草渐渗系鉴定和精细定位籽粒蛋白含量主效qGPC4D

doi:10.1016/j.jia.2024.07.029

摘要/Abstract

摘要： 小麦是一种重要的粮食作物，对粮食安全和人类健康至关重要。提高小麦品质已成为育种家满足市场日益增长的需求的重要任务。本研究以高产小麦品种济麦22和粗山羊草Y215为亲本构建了一套小麦-粗山羊草渐渗系。利用55K SNP芯片鉴定群体基因型并筛选出2727个高质量的SNP，同时测定两个环境下的籽粒品质相关性状。通过QTL分析鉴定出8个与籽粒蛋白质含量、淀粉含量和湿面筋含量相关的QTL。其中，qGPC4D为具有环境稳定性的主效QTL，其有利等位基因来自粗山羊草Y215。随后，利用渐渗系进一步精细定位，将qGPC4D缩小到9.88 Mb的物理区间内，并开发了三个连锁的KASP标记。本试验为小麦优质育种提供了重要的候选基因、优良的渐渗系和连锁的KASP标记。

Abstract:

Wheat is a staple cereal crop that is crucial for food security and human health. Improving wheat quality has become an essential task for allowing breeders to meet escalating market demand. In this study, a set of wheat–Aegilops tauschii introgression lines was developed from a cross between the high-yielding wheat variety Jimai 22 and Ae. tauschii Y215. A high-density genetic map containing 2,727 single nucleotide polymorphisms (SNPs) was constructed using a 55K SNP array, and it was used to conduct a quantitative trait loci (QTLs) analysis for grain quality-related traits. Eight QTLs were identified for grain protein content (GPC), starch content, and wet gluten content in two environments. Among them, a major and environmentally stable QTL for GPC, qGPC4D, was identified, with favorable alleles contributed by Ae. tauschii Y215. Subsequently, qGPC4D was narrowed down to a 9.88 Mb physical interval through further fine mapping utilizing the introgression lines. In addition, three linked SNP of qGPC4D were converted into high-throughput kompetitive allele-specific PCR (KASP) markers and validated in the introgression population. These findings offer promising candidate genes, elite introgression lines, and KASP markers for high-quality wheat breeding.

Key words: wheat , Aegilops tauschii , protein content , QTL , KASP

Yijun Wang, Jinhao Han, Tenglong Zhang, Mengjia Sun, Hongyu Ren, Cunyao Bo, Yuqing Diao, Xin Ma, Hongwei Wang, Xiaoqian Wang. 利用小麦-粗山羊草渐渗系鉴定和精细定位籽粒蛋白含量主效qGPC4D[J]. Journal of Integrative Agriculture, 2026, 25(5): 1813-1821.

Yijun Wang, Jinhao Han, Tenglong Zhang, Mengjia Sun, Hongyu Ren, Cunyao Bo, Yuqing Diao, Xin Ma, Hongwei Wang, Xiaoqian Wang. Identification and fine mapping of a major QTL for grain protein content, qGPC4D, using wheat–Aegilops tauschii introgression lines[J]. Journal of Integrative Agriculture, 2026, 25(5): 1813-1821.

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