新型多重耐药性整合结合元件ICEPmu3在多杀性巴氏杆菌中的鉴定

doi:10.1016/j.jia.2024.07.008

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (11): 3938-3942.DOI: 10.1016/j.jia.2024.07.008

新型多重耐药性整合结合元件ICEPmu3在多杀性巴氏杆菌中的鉴定

收稿日期:2023-11-16 接受日期:2024-04-22 出版日期:2024-11-20 发布日期:2024-10-10

Emergence of a novel multi-resistance-mediating integrative and conjugative element ICEPmu3 in Pasteurella multocida

Jiao He¹, Zhishuang Yang¹, Mingshu Wang^{1, 3, 4}, Renyong Jia^{1, 3, 4}, Shun Chen^{1, 2, 3, 4}, Mafeng Liu^{1, 2, 3, 4}, Xinxin Zhao^{1, 2, 3, 4}, Qiao Yang^{1, 2, 3, 4}, Ying Wu^{1, 2, 3, 4}, Shaqiu Zhang^{1, 2, 3, 4}, Juan Huang^{1, 2, 3, 4}, Xumin Ou^{1, 2, 3, 4}, Di Sun^{1, 2, 3, 4}, Bin Tian^{1, 2, 3, 4}, Yu He^{1, 2, 3, 4}, Zhen Wu^{1, 2, 3, 4}, Anchun Cheng^{1, 2, 3, 4#}, Dekang Zhu^{1, 2, 3, 4#}

¹ Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China
² Key Laboratory of Agricultural Bioinformatics, Ministry of Education, Chengdu 611100, China
³ International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 610199, China
⁴ Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education, Chengdu 610199, China

Received:2023-11-16 Accepted:2024-04-22 Online:2024-11-20 Published:2024-10-10
About author:Jiao He, Tel: +86-15680737990, E-mail: hejiaovet@163.com; #Correspondence Anchun Cheng, E-mail: chenganchun@vip.163.com; Dekang Zhu, E-mail: zdk24@sicau.edu.cn
Supported by:
This work was supported by the earmarked fund for China Agriculture Research System (CARS-42-17), and the Sichuan Veterinary Medicine and Drug Innovation Group of China Agricultural Research System (SCCXTD-2021-18).

摘要/Abstract

摘要：

本研究从中国鸭中分离出一株高致病性且多重耐药的多杀性巴氏杆菌（Pasteurella multocida，PM）HN141014。基于HN141014的全基因组生物信息学分析及共轭转移试验，鉴定出一种新型的多重耐药整合接合元件（ICEPmu3）。该元件属于ICEHin1056亚家族，具有种内种间转移特性，包含除tfc1和tfc20外几乎完整的Ⅳ型分泌系统，携带tetR(B)-tet(B)-tetC、aph(3ʹ)-Ia和sul2-strA-strB等多种耐药基因。进一步分析发现，ICEPmu3还存在于亚洲和美洲源的其他3株PM和8株禽类芽孢杆菌中。这些结果表明ICEPmu3的出现和流行可能会加剧养禽业中PM的防控难度。

Jiao He, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Bin Tian, Yu He, Zhen Wu, Anchun Cheng, Dekang Zhu. 新型多重耐药性整合结合元件ICEPmu3在多杀性巴氏杆菌中的鉴定[J]. Journal of Integrative Agriculture, 2024, 23(11): 3938-3942.

Jiao He, Zhishuang Yang, Mingshu Wang, Renyong Jia, Shun Chen, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Juan Huang, Xumin Ou, Di Sun, Bin Tian, Yu He, Zhen Wu, Anchun Cheng, Dekang Zhu. Emergence of a novel multi-resistance-mediating integrative and conjugative element ICEPmu3 in Pasteurella multocida[J]. Journal of Integrative Agriculture, 2024, 23(11): 3938-3942.

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新型多重耐药性整合结合元件ICEPmu3在多杀性巴氏杆菌中的鉴定

Emergence of a novel multi-resistance-mediating integrative and conjugative element ICEPmu3 in Pasteurella multocida

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