E93对棉铃虫幼虫到蛹的变态和对翅与卵巢发育的影响及其在RNAi害虫防治中的潜能研究

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (5): 1916-1929.DOI: 10.1016/j.jia.2024.06.015

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E93对棉铃虫幼虫到蛹的变态和对翅与卵巢发育的影响及其在RNAi害虫防治中的潜能研究

收稿日期:2024-04-03 修回日期:2024-06-27 接受日期:2024-05-30 出版日期:2025-05-20 发布日期:2025-04-18

dsHaE93 shows a high potential for the pest control of Helicoverpa armigera by inhibiting larval-pupal metamorphosis and development of wing and ovary

Kai Wang, Longlong Sun, Mengdan Zhang, Shuting Chen, Guiying Xie, Shiheng An, Wenbo Chen^#, Xincheng Zhao^#

Henan International Joint Laboratory of Green Pest Control, College of Plant Protection, Henan Agricultural University, Zhengzhou 450046, China

Received:2024-04-03 Revised:2024-06-27 Accepted:2024-05-30 Online:2025-05-20 Published:2025-04-18
About author:Kai Wang, E-mail: wangkai990115@163.com; #Correspondence Wenbo Chen, E-mail: wenbochen@henau.edu.cn; Xincheng Zhao, E-mail: xincheng@henau.edu.cn
Supported by:
This work was supported by the National Natural Science Foundation of China (32001912 and 32370525) and the Henan Provincial Natural Science Foundation, China (232300420012).

摘要/Abstract

摘要：

在模式昆虫中，已有研究证明蜕皮激素诱导的转录因子E93在昆虫的变态发育过程中，如幼虫组织重塑和成虫组织形成等，发挥多种作用。敲低E93基因后，可导致昆虫无法完成变态发育过程，表明E93是害虫防治的潜在靶标基因。在本项研究中，首次鉴定了棉铃虫HaE93基因，发现HaE93基因在棉铃虫卵期、预蛹期和蛹期均高表达。注射dsHaE93后，约为60%的棉铃虫在幼虫到蛹期死亡。约30%的棉铃虫可以化蛹，但化蛹时间延迟，且羽化后翅和卵巢畸形，这些结果表明干扰HaE93基因导致约90%的个体不能繁殖后代。干扰HaE93基因后，qRT-PCR测定显示蜕皮激素响应基因、几丁质合成相关基因、翅发育和卵巢发育相关基因的表达水平显著下调。这些结果表明HaE93通过调控发育相关基因的表达参与调控棉铃虫的变态以及表皮、翅和卵巢的发育。最后，通过饲喂棉铃虫dsHaE93，结果显示棉铃虫死亡率和表型与注射dsHaE93的相似，这表明HaE93可作为RNAi方法的靶基因来防控棉铃虫。

Abstract:

The ecdysone-induced transcription factor E93 in model insects plays multiple roles in the insect metamorphosis processes, such as remodeling larval tissues and determining adult tissue formation. The knockdown of E93 in insects leads to incomplete metamorphosis, suggesting that E93 is a potential target for pest control. In this study, the HaE93 gene in the cotton bollworm Helicoverpa armigera, a polyphagous pest of various commercial crops worldwide, was identified and found to have high expression in the egg, prepupal, and pupal stages. The injection of dsHaE93 induced about 60% mortality in H. armigera at the larval-pupal stage. About 30% survived but showed delayed pupation and abnormal wings, and the females developed reduced ovaries. Therefore, about 90% of the HaE93 knockdown individuals failed to reproduce before they died. The results of qRT-PCR showed that the expression levels of ecdysone primary-response genes, chitin synthesis-related genes, and wing and ovary development-related genes were reduced in HaE93 knockdown H. armigera. These results indicated that HaE93 plays a critical role in larva-pupa-adult metamorphosis and the development of the cuticle, wing, and ovary in female H. armigera by regulating the expression of the associated genes. Bioassays of dsHaE93 administered by either oral delivery or injection showed similar knockdown results, which suggested that HaE93 can be used as a target gene for the RNAi control of the pest H. armigera.

Key words: Helicoverpa armigera , transcription factor E93 , RNAi , development and metamorphosis , wing and ovary development

Kai Wang, Longlong Sun, Mengdan Zhang, Shuting Chen, Guiying Xie, Shiheng An, Wenbo Chen, Xincheng Zhao. E93对棉铃虫幼虫到蛹的变态和对翅与卵巢发育的影响及其在RNAi害虫防治中的潜能研究[J]. Journal of Integrative Agriculture, 2025, 24(5): 1916-1929.

Kai Wang, Longlong Sun, Mengdan Zhang, Shuting Chen, Guiying Xie, Shiheng An, Wenbo Chen, Xincheng Zhao. dsHaE93 shows a high potential for the pest control of Helicoverpa armigera by inhibiting larval-pupal metamorphosis and development of wing and ovary[J]. Journal of Integrative Agriculture, 2025, 24(5): 1916-1929.

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