Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (10): 3311-3327.DOI: 10.1016/j.jia.2024.05.025

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提高富甘氨酸蛋白编码基因GhGRPL的表达可以通过促进次生壁的发育增强植物对非生物和生物胁迫的抗性

  

  • 收稿日期:2024-01-04 接受日期:2024-04-23 出版日期:2024-10-20 发布日期:2024-09-10

Upregulation of the glycine-rich protein-encoding gene GhGRPL enhances plant tolerance to abiotic and biotic stressors by promoting secondary cell wall development

Wanting Yu1*, Yonglu Dai1*, Junmin Chen1, Aimin Liang1, Yiping Wu1, Qingwei Suo1, Zhong Chen1, Xingying Yan1, Chuannan Wang1, Hanyan Lai1, Fanlong Wang1, Jingyi Zhang1, Qinzhao Liu1, Yi Wang1, Yaohua Li1, Lingfang Ran1, Jie Xiang1, Zhiwu Pei1, Yuehua Xiao1, 2, 3, 4#, Jianyan Zeng1, 2, 3, 4#   

  1. 1 College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China
    2 Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, China
    3 Chongqing Key Laboratory of Crop Molecular Improvement, Chongqing 400715, China
    4 Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China
  • Received:2024-01-04 Accepted:2024-04-23 Online:2024-10-20 Published:2024-09-10
  • About author:#Correspondence Jianyan Zeng, Tel: +86-23-68251883, E-mail: zengjianyan@swu.edu.cn; Yuehua Xiao, Tel: +86-23-68250042, E-mail: xiaoyuehua@swu.edu.cn * These authors contributed equally to this study.
  • Supported by:
    This work was supported by the Special Fund for the Youth Team of the Southwest Universities, China (SWU-XJPY 202306), the Fundamental Research Funds for the Central Universities, China (SWU-KR23009), and the National Natural Sciences Foundation of China (U2003209 and 31871539).

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摘要：

非生物和生物胁迫因子均对植物的生存、生物量生成和作物产量产生不利影响。随着全球可耕地的减少和全球变暖影响的加剧，这些胁迫因子对农业生产力的冲击将会日益严重。目前，全面增强植物对非生物和生物胁迫因子的抗性仍然充满挑战。次生壁在增强植物的抗胁迫能力中起着至关重要的作用。为了通过遗传操纵次生壁增加植物的抗胁迫能力，我们从棉花纤维中克隆了一个名为类富甘氨酸蛋白（GhGRPL）的细胞壁蛋白，该蛋白在纤维细胞次生壁合成期间特异性表达。值得注意的是，该蛋白质与其拟南芥同源蛋白AtGRP不同，其富甘氨酸结构域缺失部分甘氨酸残基。GhGRPL参与次生壁的沉积，上调GhGRPL的表达可以促进木质素的积累，从而增加次生壁的厚度，进而增强植物对包括干旱和盐胁迫在内的非生物胁迫因子以及包括大丽轮枝菌（V. dahliae）侵染在内的生物胁迫的抵抗力。相反，干扰棉花中GhGRPL的表达会减少木质素的积累并削弱这种抗性。总之，我们的研究结果揭示了GhGRPL在调控次生壁发育中的作用，特别是通过影响木质素的沉积，从而增强细胞壁的坚固性和不透水性方面。这些发现突显了将来利用GhGRPL在提高植物应对非生物和生物胁迫抗性中的前景。

Abstract:

Abiotic and biotic stressors adversely affect plant survival, biomass generation, and crop yields.  As the global availability of arable land declines and the impacts of global warming intensify, such stressors may have increasingly pronounced effects on agricultural productivity.  Currently, researchers face the overarching challenge of comprehensively enhancing plant resilience to abiotic and biotic stressors.  The secondary cell wall plays a crucial role in bolstering the stress resistance of plants.  To increase plant resistance to stress through genetic manipulation of the secondary cell wall, we cloned a cell wall protein designated glycine-rich protein-like (GhGRPL) from cotton fibers, and found that it is specifically expressed during the period of secondary cell wall biosynthesis.  Notably, this protein differs from its Arabidopsis homolog, AtGRP, since its glycine-rich domain is deficient in glycine residues.  GhGRPL is involved in secondary cell wall deposition.  Upregulation of GhGRPL enhances lignin accumulation and, consequently, the thickness of the secondary cell walls, thereby increasing the plant’s resistance to abiotic stressors, such as drought and salinity, and biotic threats, including Verticillium dahliae infection.  Conversely, interference with GhGRPL expression in cotton reduces lignin accumulation and compromises that resistance.  Taken together, our findings elucidate the role of GhGRPL in regulating secondary cell wall development through its influence on lignin deposition, which, in turn, reinforces cell wall robustness and impermeability.  These findings highlight the promising near-future prospect of adopting GhGRPL as a viable, effective approach for enhancing plant resilience to abiotic and biotic stress factors.


Key words: glycine-rich protein-like , secondary cell wall ,  abiotic stress ,  biotic stress ,  stress resistance