梨树腐烂病菌代谢产物和菌丝生长提高梨火疫病菌致病性

doi:10.1016/j.jia.2024.05.020

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (9): 3045-3054.DOI: 10.1016/j.jia.2024.05.020

梨树腐烂病菌代谢产物和菌丝生长提高梨火疫病菌致病性

收稿日期:2023-11-30 接受日期:2024-04-17 出版日期:2024-09-20 发布日期:2024-08-21

Cytospora pyri promotes Erwinia amylovora virulence by providing metabolites and hyphae

Tong Shen^{1, 2}, Mengdi Ye³, Yeping Xu^{1, 2}, Bohan Ding^{1, 2}, Hongtao Li^{1, 2}, Li Zhang³, Jun Wang³, Yanli Tian^{1, 2, 4}, Baishi Hu^{1, 2, 4, 5#}, Youfu Zhao⁶

¹ College of Plant Protection/Key Laboratory of Integrated Management of Crop Diseases and Pests, Nanjing Agricultural University, Nanjing 210095, China
² Key Laboratory of Plant Quarantine Pests Monitoring and Control, Ministry of Agriculture and Rural Affairs, Nanjing 210095, China
³Plant Protection and Quarantine Station of Xinjiang Uygur Autonomous Region, Urumqi 830001, China
⁴Xinjiang Key Laboratory of Agricultural Biosafety, Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830013, China
⁵College of Agriculture, Xinjiang Agricultural University, Urumqi 830052, China
⁶ Plant Pathology Department, Washington State University, Prosser, WA 24106, USA

Received:2023-11-30 Accepted:2024-04-17 Online:2024-09-20 Published:2024-08-21
About author:Tong Shen, E-mail: 18331519385@163.com; #Correspondence Baishi Hu, Tel/Fax: +86-25-84395240, E-mail: hbs@njau.edu.cn
Supported by:
This work was supported by the Major Science and Technology Projects in Xinjiang, China (2023A02006).

摘要/Abstract

摘要：

细菌-真菌互作广泛存在于自然界中。先前已有许多关于植物病原细菌与病原真菌互作的研究，但对梨树病原细菌与病原真菌的互作研究却鲜有报道。本团队在新疆库尔勒香梨园内发现梨腐烂病发生严重的梨园内常伴随梨火疫病的出现。然而这两种病原物在侵染梨树期间的关系尚不清楚。因此，本研究目的在于明确梨火疫病菌（Erwinia amylovora）与梨腐烂病菌（Cytospora pyri）之间是否存在互作以及探究其潜在的协同致病机制。本研究首先对采集到的梨树样品进行病原物检测，计算两种病原物的田间共存率；随后对E. amylovora和C. pyri进行了室内离体接种，观测病害的发生情况；最后通过对生长、物理互作、毒力因子产生及毒力因子编码基因表达的监测，解析E. amylovora与C. pyri互作的分子机制。结果表明，E. amylovora与C. pyri可在同一病斑中共存，且在离体梨树组织上同时接种两种病原菌可加重病害的发生。同时发现，C. pyri虽不影响E. amylovora的生长，却可以促进E. amylovora的扩散及毒力因子梨火疫毒素的产生。本研究结果表明，E. amylovora与C. pyri在侵染梨树期间存在协同致病作用，C. pyri可通过菌丝和代谢产物促进E. amylovora的扩散并提高致病力，从而加重病害的发生。本文是对梨树病原物E. amylovora与C. pyri互作研究的首次报道，开展梨树病原细菌与病原真菌的协同致病研究，对明确病原物的侵染机制，保证新疆梨产业平稳健康发展具有重要意义。

Abstract:

Bacterial–fungal interactions are widespread in nature. We observed that pear orchards affected by Cytospora pyri (formerly Valsa pyri) were often accompanied with Erwinia amylovora. However, the relationship of the two pathogens was unclear. The objective of this study was to determine whether the synergistic effect exists between E. amylovora and C. pyri. We first analyzed the coexistence frequencies of E. amylovora and C. pyri in pear trees. Virulence of the two pathogens, growth, physical interactions, amylovoran production, and expression of genes for amylovoran biosynthesis were conducted. Our results showed that E. amylovora and C. pyri could coexist on the same lesion and caused much more severe disease. We also found that E. amylovora could physically attach to C. pyri and the expression of amylovoran biosynthesis genes were up-regulated with fungal metabolite treatment. These results indicate that E. amylovora and C. pyri can cooperatively interact, which provides C. pyri with an opportunity to promote bacterial dispersal and production of virulence factor in E. amylovora.

Key words: pear fire blight , Erwinia amylovora , Valsa canker , amylovoran , synergy

Tong Shen, Mengdi Ye, Yeping Xu, Bohan Ding, Hongtao Li, Li Zhang, Jun Wang, Yanli Tian, Baishi Hu, Youfu Zhao. 梨树腐烂病菌代谢产物和菌丝生长提高梨火疫病菌致病性[J]. Journal of Integrative Agriculture, 2024, 23(9): 3045-3054.

Tong Shen, Mengdi Ye, Yeping Xu, Bohan Ding, Hongtao Li, Li Zhang, Jun Wang, Yanli Tian, Baishi Hu, Youfu Zhao. Cytospora pyri promotes Erwinia amylovora virulence by providing metabolites and hyphae[J]. Journal of Integrative Agriculture, 2024, 23(9): 3045-3054.

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梨树腐烂病菌代谢产物和菌丝生长提高梨火疫病菌致病性

Cytospora pyri promotes Erwinia amylovora virulence by providing metabolites and hyphae

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