RPS27L基因启动子的插入缺失突变调节猪骨骼肌生长

doi:10.1016/j.jia.2024.05.005

摘要/Abstract

摘要： 产肉性状的遗传改良一直是猪育种的主要目标。地理隔离、自然和人工选择导致中国本地猪和西方商品猪在产肉性状上存在显著差异。比较基因组和转录组为挖掘与骨骼肌生长发育相关遗传变异和基因提供了强有力工具。然而，目前可利用的遗传变异和基因的数量仍然非常有限。在本研究中，利用中外猪种的比较转录组数据发现，核糖体蛋白S27样（RPS27L）基因在猪的骨骼肌中高表达，且在中国本地猪骨骼肌中的表达水平显著高于西方商品猪。细胞功能实验结果表明，过表达RPS27L促进了猪骨骼成肌细胞的增殖并抑制其分化。相反，RPS27L的敲低则抑制了该肌细胞的增殖并促进了分化。有趣的是，在RPS27L启动子区域发现了一个13bp的插入缺失(InDel)变异，该变异序列在中国本地猪品种中呈现插入型，在西方商品猪中则主要呈现缺失型。双荧光素酶报告实验进一步表明，该InDel通过影响转录因子3(TCF3)和肌源性分化抗原(MYOD)与启动子的结合调控RPS27L的表达水平。此外，关联分析显示RPS27L的表达水平与猪背膘厚呈正相关。同时，该InDel还与猪240日龄时体重呈显著负相关。综上，本研究表明RPS27L是调节猪骨骼肌生长发育的潜在基因，并可能作为猪产肉性状改良的候选标记。本研究不仅为动物育种提供了新的分子标记，还有助于了解人类骨骼肌的发育以及相关疾病的发生。

Abstract:

Genetic improvement of meat production traits has always been the primary goal of pig breeding. Geographical isolation, natural and artificial selection led to significant differences in the phenotypes of meat production traits between Chinese local pigs and Western commercial pigs. Comparative genomics and transcriptomics analysis provided powerful tools to identify genetic variants and genes associated with skeletal muscle growth. However, the number of available genetic variants and genes are still limited. In this study, a comprehensive comparison of transcriptomes showed that ribosomal protein S27-like (RPS27L) gene was highly expressed in skeletal muscle and up-regulated in Chinese local pigs when compared with Western commercial pigs. Functional analysis revealed that overexpression of RPS27L promoted myoblast proliferation and repressed differentiation in pig skeletal muscle cells. Conversely, the knockdown of RPS27L led to the inhibition of myoblast proliferation and the promotion of differentiation. Notably, a 13-bp insertion-deletion (InDel) mutation was identified within the RPS27L promoter, inserted in Chinese local breeds and predominantly deleted in Western commercial breeds. Luciferase reporter assay suggested this InDel modulated RPS27L expression by influencing transcription factor 3 (TCF3) and myogenic differentiation antigen (MYOD) binding to promoter. Furthermore, a positive correlation was observed between the expression of RPS27L expression and backfat thickness. Association studies demonstrated this InDel was significantly associated with the body weight of pigs at the age of 240 days. Together, our results suggested that RPS27L was a regulator of skeletal muscle development and growth, and was a candidate marker for improving meat production traits in pigs. This study not only provided a biomarker for animal breeding, but also was helpful for understanding skeletal muscle development and muscle-related disease in humans.

Key words: pig , InDel , , RPS27L , , skeletal muscle , , growth and development

. RPS27L基因启动子的插入缺失突变调节猪骨骼肌生长[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.05.005.

Xiaoqin Liu, Xinhao Fan, Junyu Yan, Longchao Zhang, Lixian Wang, Honor Calnan, Yalan Yang, Graham Gardner, Rong Zhou, Zhonglin Tang. An InDel in the Promoter of Ribosomal Protein S27-like Gene Regulates Skeletal Muscle Growth in Pigs[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.05.005.

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