崂山奶山羊泌乳期内乳脂肪球膜蛋白的变化研究

doi:10.1016/j.jia.2024.03.080

摘要/Abstract

摘要： 乳脂肪球膜（MFGM）结构复杂且具有多种功能，其组成受多种因素的影响。然而，目前关于整个泌乳期山羊乳脂肪球膜蛋白质组的系统研究很少。本研究共采集了来源于15只健康奶山羊的6个泌乳时间点的样品，采用数据独立采集（DIA）和数据依赖采集（DDA）两种方法，结合多元统计分析，对山羊MFGM蛋白质组进了分析。借助DIA方法，在整个泌乳期共鉴定出890种差异表达的MFGM蛋白。从1天到240天，嗜乳脂蛋白亚家族1成员A1、脂蛋白脂肪酶、围脂滴蛋白2、甘油三酯脂肪酶表达上调，而载脂蛋白E、补体C3、簇蛋白和免疫球蛋白则下调。此外，从1到90天，膜联蛋白A1、膜联蛋白A2、抗凝血酶III表达下调，然后到240天上调。蛋白-蛋白相互作用分析表明，白蛋白具有高度的关联性，因此被认为是中心蛋白。总之，我们的研究结果为了解整个泌乳期山羊MFGM蛋白的生物学特性提供了新的见解，这将有助于改进特定MFGM产品及婴儿配方奶粉的生产。

Abstract: The milk fat globule membrane (MFGM) is a complex structure with numerous functions, and its composition is affected by a number of circumstances. There have been few systematic investigations on goat MFGM proteome profiling during lactation. Individual milk samples from 15 healthy dairy goats were obtained at six lactation time points for investigation of the MFGM proteome using both data-independent acquisition (DIA) and data-dependent acquisition (DDA) proteomics techniques combined with multivariate statistical analysis. Using the DIA method, 890 variably abundant MFGM proteins were discovered throughout the lactation cycle. From 1 to 240 d, butyrophilin subfamily 1 member A1, lipoprotein lipase, perilipin-2, and adipose triglyceride lipase were upregulated, while APOE, complement C3, clusterin, and IgG were downregulated. Furthermore, from 1 to 90 d, annexin A1, annexin A2, and antithrombin-III were downregulated, then upregulated by d 240. Albumin had a high degree of connectedness, indicating that it was a key protein, according to protein-protein interaction research. Overall, our findings gave new insights into the biological features of MFGM protein in goat milk throughout lactation, which may aid in the creation of specialized MFGM products and infant formula.

Key words: goat , milk fat globule membrane protein , , data-independent acquisition , , data-dependent acquisition , , lactation , , proteomics

. 崂山奶山羊泌乳期内乳脂肪球膜蛋白的变化研究[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.03.080.

Chuozi Liang, Zhongna Yu, Guangming Zhu, Yixuan Li, Xueheng Sun, Hongning Jiang, Qijing Du, Rongbo Fan, Jun Wang, Yongxin Yang, Rongwei Han. Changes in milk fat globule membrane proteins along lactation stage of Laoshan dairy goat[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.03.080.

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