EjGASA6通过增强赤霉素生物合成促进开花和根伸长

doi:10.1016/j.jia.2024.03.066

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (5): 1568-1579.DOI: 10.1016/j.jia.2024.03.066

EjGASA6通过增强赤霉素生物合成促进开花和根伸长

修回日期:2023-03-30 接受日期:2023-11-02 出版日期:2024-05-20 发布日期:2024-04-23

EjGASA6 promotes flowering and root elongation by enhancing gibberellin biosynthesis

Qian Chen^{1, 2*}, Shunyuan Yong^{1, 2*}, Fan Xu³, Hao Fu^1,
2, Jiangbo Dang^{1, 2}, Qiao He^{1, 2}, Danlong Jing^1,
2, Di Wu^{1, 2}, Guolu Liang^{1, 2}, Qigao Guo^{1, 2#}

¹Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River (Ministry of Education)/College of Horticulture and Landscape Architecture, Southwest University, Chongqing 400715, China
²State Cultivation Base of Crop Stress Biology for Southern Mountainous Land of Southwest University/Academy of Agricultural Sciences of Southwest University, Chongqing 400715, China
³College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, China

Revised:2023-03-30 Accepted:2023-11-02 Online:2024-05-20 Published:2024-04-23
About author:Qian Chen, E-mail: chenqiansuaige@163.com; Shunyuan Yong, E-mail: Yongshunyuan2022@163.com; #Correspondence Qigao Guo, E-mail: qgguo@126.com * These authors contributed equally to this study.
Supported by:
This work was financially supported by the National Key R&D Program of China (2023YFD1600800), the National Nature Science Foundation of China (32102321), the Chongqing Science and Technology Commission, China (cstc2024ycjh-bgzxm0202, cstc2021jscx-gksbX0010 and cstc2021jcyj-msxmX1156), the Chongqing Forestry Administration, China (YuLinKeYan2022-14), the Innovation Research Group Funds for Chongqing Universities, China (CXQT19005), the Characteristic Fruit Industry and Technology System Innovation Team of Chongqing Agriculture and Rural Affairs Commission, China [(2022)164 and 2020(3)01], and the Chongqing Postgraduate Research and Innovation Programme, China (CYB23128).

摘要/Abstract

摘要： Gibberellic Acid-Stimulated Arabidopsis（GASA）基因家族广泛参与了基因表达调控和植物生长发育及胁迫反应。为了研究枇杷GASA基因在植物生长发育调控中的功能，我们克隆了一个与拟南芥AtGASA6同源的枇杷EjGASA6基因。通过对受赤霉素诱导的EjGASA6基因进行异源表达，发现转基因植株开花时间提前，主根变长，这些表型特征与赤霉素含量增加有关。转录组和qRT-PCR分析结果显示，编码赤霉素生物合成关键酶的GA3ox1和GA20ox1基因的表达量显著升高。此外，通过双荧光素酶实验我们证实了EjGASA6可以增强GA20ox1的启动子活性。综上，我们的研究结果表明，EjGASA6通过正调控赤霉素的生物合成来提前开花时间和促进主根伸长。该研究结果拓宽了对GASAs在植物发育和生长中的作用的认识，为进一步研究EjGASA6在枇杷生长和发育中的调节功能奠定了基础。

Abstract:

The Gibberellic Acid-stimulated Arabidopsis (GASA) gene family is involved in the regulation of gene expression and plant growth, development, and stress responses. To investigate the function of loquat GASA genes in the growth and developmental regulation of plants, a loquat EjGASA6 gene homologous to Arabidopsis AtGASA6 was cloned. EjGASA6 expression was induced by gibberellin, and ectopic transgenic plants containing this gene exhibited earlier bloom and longer primary roots since these phenotypic characteristics are related to higher gibberellin content. Transcriptome analysis and qRT-PCR results showed that the expression levels of GA3ox1 and GA20ox1, which encode key enzymes in gibberellin biosynthesis, were significantly increased. Furthermore, we confirmed that EjGASA6 could promote the expression of GA20ox1 via the luciferase reporter system. Overall, our results suggest that EjGASA6 promotes blooming and main-root elongation by positively regulating gibberellin biosynthesis. These findings broaden our understanding of the role of GASAs in plant development and growth, and lay the groundwork for future research into the functions of EjGASA6 in regulating loquat growth and development.

Key words: loquat , EjGASA6 , gibberellin synthesis , biological function

. EjGASA6通过增强赤霉素生物合成促进开花和根伸长[J]. Journal of Integrative Agriculture, 2024, 23(5): 1568-1579.

Qian Chen, Shunyuan Yong, Fan Xu, Hao Fu, Jiangbo Dang, Qiao He, Danlong Jing, Di Wu, Guolu Liang, Qigao Guo.

EjGASA6 promotes flowering and root elongation by enhancing gibberellin biosynthesis [J]. Journal of Integrative Agriculture, 2024, 23(5): 1568-1579.

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EjGASA6通过增强赤霉素生物合成促进开花和根伸长

EjGASA6 promotes flowering and root elongation by enhancing gibberellin biosynthesis

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