苦荞麦糖转运体家族的进化和表达分析揭示FtERD23在干旱胁迫下的潜在功能

doi:10.1016/j.jia.2024.03.031

摘要/Abstract

摘要： 干旱正在成为作物生产的常见威胁。为了应对这种压力并确保全球粮食安全，鉴定和利用优异的抗旱基因对于开发抗旱作物品种至关重要。然而，目前已知糖转运体参与了许多植物的抗逆性，但是苦荞麦的糖转运体基因家族尚未得到系统分析。本研究从品苦基因组中鉴定了140个糖转运体基因，并将其分为10个亚族。结构分析表明，与其他亚家族相比，SGB/pGlcT亚家族内含子数量最多。启动子区域存在丰富的非生物胁迫相关顺式作用元件。共线性分析表明，FtSUT7、FtSTP28、FtPLT1和FtINT2基因的相对较为古老。对各种非生物胁迫下糖转运基因的表达进行了筛选，结果表明FtERD23、FtINT2、FtpGlcT2和FtSTP27可能与苦荞抗逆性有关。此外，还观察到FtERD23的过表达也许是通过葡萄糖转运维持渗透压，从而增强干旱胁迫耐受性。此外，利用WGCNA和FCMA进行的基因共表达分析发现了六个可能调控FtERD23表达并参与植物抗旱的转录因子。总之，这一系统分析为进一步确定糖转运体基因的功能特性以提高苦荞麦及其相关物种的耐旱性提供了理论依据。

Abstract: Drought is becoming a common threat to crop production. To combat this stress and ensure global food security, the identification and utilization of excellent drought-resistant genes are crucial for developing drought-resistant crop varieties. However, sugar transporters are known to be involved in stress tolerance in many plants, while the sugar transporter gene family of Tartary buckwheat has not been systematically analyzed yet. In this study, 140 sugar transporter genes were identified from the ‘Pinku’ Tartary buckwheat genome and classified into ten subfamilies. Structural analysis showed that subfamily SGB/pGlcT had the highest number of introns compared to other subfamilies, and abundant abiotic stress-related cis-acting elements existed in the promoter region. Collinear analysis revealed that FtSUT7, FtSTP28, FtPLT1, and FtINT2 genes are relatively ancient. The expression of sugar transporter genes was screened under various abiotic stresses which revealed the association of stress tolerance with different sugar transporter genes, i.e., FtERD23, FtINT2, FtpGlcT2, and FtSTP27. Further, it was observed that the overexpression of FtERD23 maintains osmotic pressure through glucose transport, which may enhance drought stress tolerance. Moreover, gene co-expression analyses using WGCNA and FCMA identified six transcription factors that may regulate FtERD23 expression and are involved in plant drought tolerance. In summary, this systematic analysis provides a theoretical basis for further functional characterization of sugar transporter genes to improve drought tolerance in Tartary buckwheat and its related species.

Key words: Tartary buckwheat , drought tolerance , , sugar transporter

. 苦荞麦糖转运体家族的进化和表达分析揭示FtERD23在干旱胁迫下的潜在功能[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.03.031.

Dili Lai, Md. Nurul Huda, Yawen Xiao, Tanzim Jahan, Wei Li, Yuqi He, Kaixuan Zhang, Jianping Cheng, Jingjun Ruan, Meiliang Zhou. Evolutionary and expression analysis of sugar transporters from Tartary buckwheat revealed the potential function of FtERD23 in drought stress[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.03.031.

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