SbTEF1基因的自然变异对高粱幼苗耐盐性的影响

doi:10.1016/j.jia.2024.03.030

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (11): 4168-4181.DOI: 10.1016/j.jia.2024.03.030

SbTEF1基因的自然变异对高粱幼苗耐盐性的影响

收稿日期:2023-11-14 修回日期:2024-03-06 接受日期:2024-01-03 出版日期:2025-11-20 发布日期:2025-10-11

Natural variation in SbTEF1 contributes to salt tolerance in sorghum seedlings

Chang Liu¹, Lei Tian¹, Wenbo Yu¹, Yu Wang¹, Ziqing Yao¹, Yue Liu², Luomiao Yang³, Chunjuan Liu¹, Xiaolong Shi¹, Tao Liu⁴, Bingru Chen⁵, Zhenguo Wang⁶, Haiqiu Yu^{1, 7#}, Yufei Zhou^1#

¹College of Agriculture, Shenyang Agricultural University, Shenyang 110866, China
²Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang 110866, China
³College of Agriculture, Northeast Agricultural University, Harbin 150006, China
⁴Institute of Sorghum Research, Baicheng Academy of Agricultural Sciences, Baicheng 137000, China
⁵Institute of Sorghum Research, Jilin Academy of Agricultural Sciences, Changchun 130033, China
⁶Institute of Sorghum Research, Tongliao Agriculture and Animal Husbandry Sciences, Tongliao 028000, China
⁷Liaoning Agricultural Vocational and Technical College, Yingkou 115009, China

Received:2023-11-14 Revised:2024-03-06 Accepted:2024-01-03 Online:2025-11-20 Published:2025-10-11
About author:#Correspondence Yufei Zhou, Tel: +86-24-88487135, E-mail: zhouyufei@syau.edu.cn; Haiqiu Yu, Tel: +86-24-88487135, E-mail: yuhaiqiu@syau.edu.cn
Supported by:
This work was supported by the Science and Technology Program in Liaoning Province, China (2022-BS-172), the China Agriculture Research System of MOF and MARA (CARS-06-14.5-A17) and the Guiding Funds of the Central Government for the Local Scientific and Technological Development Basic Research Program of Jilin Province, China (202002068JC).

摘要/Abstract

摘要： 盐胁迫是影响作物产量和品质的主要非生物胁迫因素。由于耐盐基因资源的匮乏，耐盐育种的进程受到了极大的限制。高粱是一种具有较强盐碱耐受性的作物，挖掘其耐盐基因显得尤为重要。本研究对186份核心高粱种质材料进行重测序，结合盐胁迫下相对根长及根鲜重表型进行全基因组关联分析。结果显示，在多重耐盐表型共定位区间内共有8个候选基因，结合数据库注释及盐胁迫下基因表达结果，SbTEF1（一种编码转录延长因子蛋白的基因）被确定为耐盐候选基因。在此基础上，结合连锁不平衡分析、基因克隆、基因单倍型分析及等位基因效应分析，发现位于SbTEF1启动子区域的PAV284通过调节盐胁迫情况下基因表达影响高粱的耐盐性。PAV284可作为分子标记，为高粱种质的耐盐改良及高粱耐盐品种培育提供技术支撑。

Abstract:

Salt stress is a major constraint to crop productivity and quality. The limited availability of salt-tolerant genes poses significant challenges to breeding programs aimed at enhancing salt tolerance. Sorghum displays a remarkable ability to withstand saline conditions; therefore, elucidating the genetic underpinnings of this trait is crucial. This study entailed a comprehensive resequencing of 186 sorghum accessions to perform a genome-wide association study (GWAS) focusing on relative root length (RL) and root fresh weight (RFW) under salt stress conditions. We identified eight candidate genes within a co-localized region, among which SbTEF1 - a gene encoding a transcription elongation factor protein - was deemed a potential candidate due to its annotation and expression pattern alterations under salt stress. Haplotype analysis, gene cloning, linkage disequilibrium (LD) analysis, and allele effect analysis revealed that PAV284, located in the promoter region of SbTEF1, modulated gene expression under salt stress, which, in turn, influenced sorghum seedlings’ salt tolerance. PAV284 holds promise as a genetic marker for selecting salt-tolerant germplasm via marker-assisted breeding, enhancing the development of salt-tolerant sorghum cultivars.

Key words: salt stress , GWAS , transcription elongation factor , sorghum (Sorghum biocolor L.)

Chang Liu, Lei Tian, Wenbo Yu, Yu Wang, Ziqing Yao, Yue Liu, Luomiao Yang, Chunjuan Liu, Xiaolong Shi, Tao Liu, Bingru Chen, Zhenguo Wang, Haiqiu Yu, Yufei Zhou. SbTEF1基因的自然变异对高粱幼苗耐盐性的影响[J]. Journal of Integrative Agriculture, 2025, 24(11): 4168-4181.

Chang Liu, Lei Tian, Wenbo Yu, Yu Wang, Ziqing Yao, Yue Liu, Luomiao Yang, Chunjuan Liu, Xiaolong Shi, Tao Liu, Bingru Chen, Zhenguo Wang, Haiqiu Yu, Yufei Zhou. Natural variation in SbTEF1 contributes to salt tolerance in sorghum seedlings [J]. Journal of Integrative Agriculture, 2025, 24(11): 4168-4181.

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SbTEF1基因的自然变异对高粱幼苗耐盐性的影响

Natural variation in SbTEF1 contributes to salt tolerance in sorghum seedlings

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