大麦HvGL7-2H基因在籽粒发育中的功能及优异单倍型鉴定

doi:10.1016/j.jia.2024.03.025

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (11): 4153-4167.DOI: 10.1016/j.jia.2024.03.025

大麦HvGL7-2H基因在籽粒发育中的功能及优异单倍型鉴定

收稿日期:2023-10-16 修回日期:2024-03-06 接受日期:2024-01-02 出版日期:2025-11-20 发布日期:2025-10-11

Functional characterization and identification of superior haplotypes of barley HvGL7-2H (Hordeum vulgare L.) in grain features

Rui Liu^{1, 2*}, Hongna Cheng^{1, 3*}, Dandan Qin^1*, Le Xu³, Fuchao Xu¹, Qing Xu¹, Yanchun Peng¹, Shuangtao Ge¹, Longqing Sun¹, Guoqing Dong^2#, Jing Dong^1#

¹Key Laboratory for Crop Molecular Breeding, Ministry of Agriculture and Rural Affairs/Hubei Key Laboratory of Food Crop Germplasm and Genetic Improvement/Hubei Hongshan Laboratory/Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan 430064, China
²Wuhan Polytechnic University, Wuhan 430023, China
³Key Laboratory of Sustainable Crop Production in the Middle Reaches of the Yangtze River (Co-construction by Ministry and Province), Ministry of Agriculture and Rural Affairs/College of Agriculture, Yangtze University, Jingzhou 434023, China

Received:2023-10-16 Revised:2024-03-06 Accepted:2024-01-02 Online:2025-11-20 Published:2025-10-11
About author:#Correspondence Jing Dong, E-mail: dongjingsir061@163.com; Guoqing Dong, E-mail: donggq@whpu.edu.cn * These authors contributed equally to this study.
Supported by:
This work was financially supported by the National Natural Science Foundation of China (31771774), the National Key Research and Development Program of China (2018YFD1000700 and 2018YFD1000706), the Young Top-notch Talent Cultivation Program of Hubei Province, Hubei Hongshan Laboratory, China and the China Agriculture Research System of Ministry of Agriculture and Rural Affairs (CARS-05).

摘要/Abstract

摘要：

大麦(Hordeum vulgare L.)是世界上种植面积第四大的谷类作物，粒长、粒宽、千粒重等籽粒性状对大麦的产量和品质至关重要。粒型相关基因的克隆及优异等位变异鉴定是大麦分子标记辅助选择的基础。本研究根据已知的水稻GL7基因，从大麦中克隆出其同源基因HvGL7-2H。基于EMS诱变的大麦地方品种“哈铁系”突变体库进行该基因的功能验证，结果表明HvGL7-2H在大麦粒长形成中发挥着重要作用。利用363份大麦种质资源材料，通过基于候选基因的关联分析方法，对其优异单倍型进行了筛选。结果表明，Hap3是粒长和千粒重的优异单倍型，Hap4是千粒重的优异单倍型。综上所述，本研究所鉴定的携带HvGL7-2H优异单倍型的大麦种质资源是重要的基因资源和种质资源，所挖掘的目标性状优异单倍型也将有助于大麦粒型和产量的分子遗传改良。

Abstract:

Barley (Hordeum vulgare L.) ranks as the fourth most cultivated cereal crop globally by planting area. Kernel characteristics, including grain length, grain width, and thousand-grain weight (TGW), are essential determinants of barley yield and quality. The identification and cloning of genes related to kernel traits, along with the detection of superior alleles, are fundamental for marker-assisted selection in barley breeding. This study presents the cloning of HvGL7-2H from barley, based on the known rice GL7 gene. The functional significance of HvGL7-2H in grain length was confirmed through ethyl methane sulfonate (EMS) mutants of the barley landrace “Hatiexi”. A candidate gene-based association analysis was conducted using a panel of 363 barley accessions to identify superior haplotypes for HvGL7-2H. The analysis revealed that Hap3 represented the superior haplotype for both grain length and TGW, while Hap4 emerged as the superior haplotype for TGW. These findings indicate that genotypes carrying the superior allele serve as valuable genetic resources, and the molecular markers identified herein will facilitate grain size and yield improvement in barley breeding programs.

Key words: barley , HvGL7-2H , functional characterization , candidate gene-based association analysis , superior haplotype , grain features

Rui Liu, Hongna Cheng, Dandan Qin, Le Xu, Fuchao Xu, Qing Xu, Yanchun Peng, Shuangtao Ge, Longqing Sun, Guoqing Dong, Jing Dong. 大麦HvGL7-2H基因在籽粒发育中的功能及优异单倍型鉴定[J]. Journal of Integrative Agriculture, 2025, 24(11): 4153-4167.

Rui Liu, Hongna Cheng, Dandan Qin, Le Xu, Fuchao Xu, Qing Xu, Yanchun Peng, Shuangtao Ge, Longqing Sun, Guoqing Dong, Jing Dong. Functional characterization and identification of superior haplotypes of barley HvGL7-2H (Hordeum vulgare L.) in grain features[J]. Journal of Integrative Agriculture, 2025, 24(11): 4153-4167.

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大麦HvGL7-2H基因在籽粒发育中的功能及优异单倍型鉴定

Functional characterization and identification of superior haplotypes of barley HvGL7-2H (Hordeum vulgare L.) in grain features

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