PDL1依赖的反式作用干扰小RNA调节水稻侧生器官的极性发育

doi:10.1016/j.jia.2024.01.025

摘要/Abstract

摘要： 叶片和颖壳作为侧生器官，对光合作用和种子形态有重要影响，从而影响产量。然而，控制水稻极性发育的分子机制仍需进一步研究。本研究中，我们分离了一个水稻polarity defect of lateral organs 1 (pdl1)突变体，该突变体表现出由极性发育缺陷引起的扭曲的/丝状的叶片和裂开的/丝状的外稃。PDL1编码定位于细胞质颗粒的SUPPRESSOR OF GENE SILENCING 3蛋白。PDL1表达于茎尖分生组织、花序分生组织、花分生组织以及叶片和花器官等侧生器官。PDL1参与了tasiR-ARF的合成，可能进一步调控OsARFs的表达。同时，远轴面特征基因miR165/166和近轴面特征基因OSHBs的表达水平分别显著升高和降低。本研究结果阐明了PDL1介导的tasiR-ARF合成进而调控水稻侧生器官极性发育的分子机制。

Abstract: Leaves and glumes act as lateral organs and have essential effects on photosynthesis and seed morphology, thus affecting yield. However, the molecular mechanisms controlling their polarity development in rice is still worth further analysis. Here, we isolated a polarity defect of lateral organs 1 (pdl1) mutant in rice, which exhibits twisted/filamentous-shaped leaves and cracked/filamentous-shaped lemmas caused by defects in polarity development. PDL1 encodes a SUPPRESSOR OF GENE SILENCING 3 protein localized in the cytoplasm granules. PDL1 is expressed in the shoot apical meristem, inflorescence meristem, floral meristem, and lateral organs including leaf and floral organs. PDL1 is involved in the synthesis of tasiR-ARF, which may subsequently modulate the expression of OsARFs. Meanwhile, the expression of abaxial miR165/166 and the adaxial identity genes OSHBs was increased and decreased significantly, respectively. The results of this study clarified the molecular mechanism that the PDL1-mediated tasiR-ARF synthesis regulates the lateral organ polarity development in rice.

Key words: lateral organs , , polarity development , , ta-siRNA , , Oryza , sativa , L

. PDL1依赖的反式作用干扰小RNA调节水稻侧生器官的极性发育[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.01.025.

Yi Zhang, Jing You, Jun Tang, Wenwen Xiao, Mi Wei, Ruhui Wu, Jinyan Liu, Hanying Zong, Shuoyu Zhang, Jie Qiu, Huan Chen, Yinghua Ling, Fangming Zhao, Yunfeng Li, Guanghua He, Ting Zhang. PDL1-dependent trans-acting siRNAs regulate lateral organ polarity development in rice[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2024.01.025.

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