利用全基因组连锁分析解析中麦578/济麦22 RIL群体黑胚病抗性遗传基础

doi:10.1016/j.jia.2023.12.039

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (9): 3311-3321.DOI: 10.1016/j.jia.2023.12.039

利用全基因组连锁分析解析中麦578/济麦22 RIL群体黑胚病抗性遗传基础

收稿日期:2023-09-21 修回日期:2024-01-03 接受日期:2023-11-08 出版日期:2025-09-20 发布日期:2025-08-11

Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22

Tiantian Chen^{1, 2}, Lei Li^1,
2, Dan Liu¹, Yubing Tian^{1, 2}, Lingli Li^{1, 2}, Jianqi Zeng^{1, 2}, Awais Rasheed¹, Shuanghe Cao^{1, 2}, Xianchun Xia¹, Zhonghu He^{1, 2, 3}, Jindong Liu^{1, 2#}, Yong Zhang^{1, 2#}

¹Institute of Crop Sciences, Chinese Academy of Agricultural Sciences (CAAS)/State Key Laboratory of Crop Gene Resources and Breeding, Ministry of Science and Technology/National Wheat Improvement Center, Beijing 100081, China
²Zhongyuan Research Center, Chinese Academy of Agricultural Sciences, Xinxiang 453519, China
³International Maize and Wheat Improvement Center (CIMMYT), China Office, c/o CAAS, Beijing 100081, China

Received:2023-09-21 Revised:2024-01-03 Accepted:2023-11-08 Online:2025-09-20 Published:2025-08-11
About author:Tiantian Chen, E-mail: 2925018015@qq.com; #Correspondence Jindong Liu, Tel: +86-10-82108889, E-mail: liujindong@caas.cn; Yong Zhang, Tel: +86-10-82108745, E-mail: zhangyong05@caas.cn
Supported by:
This work was funded by the National Natural Science Foundation of China (32272186), the Beijing Natural Science Foundation, China (6242031), the Basal Research Fund of the Chinese Academy of Agricultural Sciences (CAAS) (S2022QH04), the National Key R&D Program of China (2022YFD1201500), the Young Elite Scientists Sponsorship Program by China Association for Science and Technology (YESS, 2020QNRC001), the Modern Cold and Drought Characteristic Agricultural Seed Industry Research Project-2025, Gansu Province, China (ZYGG-2025-8), the Nanfan Special Project, CAAS (YBXM2303), and the Science and Technology Innovation Program of CAAS.

摘要/Abstract

摘要： 小麦黑胚病表现为籽粒胚部呈现明显的黑褐色斑点，会降低小麦品质和商品等级。挖掘抗性遗传位点可有效提升小麦品种黑胚病抗性。本研究以中麦578/济麦22重组自交系（Recombinant inbred line, RIL）的262份家系为研究材料，利用5个环境的黑胚率表型数据与小麦50K SNP芯片构建的高密度遗传连锁图谱，定位到6个稳定的黑胚病抗性遗传位点（Quantitative trait loci, QTL），并命名为QBp.caas-2A、QBp.caas-2B1、QBp.caas-2B2、QBp.caas-2D、QBp.caas-3A和QBp.caas-5B，分别解释2.1-28.8%的表型变异。其中，QBp.caas-2B1和QBp.caas-2B2的抗性等位基因来自中麦578，其余位点抗性等位基因来自济麦22。在以上位点中，QBp.caas-2B2、QBp.caas-2D和QBp.caas-3A与之前报道的位点接近或一致，而QBp.caas-2A、QBp.caas-2B1和QBp.caas-5B可能是新的抗性位点。利用165份自然品种对5个KASP标记（Kasp_2A_BP、Kasp_2B1_BP、Kasp_2B2_BP、Kasp_3A_BP和Kasp_5B_BP）的有效性进行了验证。本研究为小麦黑胚病分子标记辅助选择育种提供了可用QTL和分子标记。

Abstract:

Black point is a black discoloration of the grain embryo that reduces the grain quality and commodity grade. Identifying the underlying genetic loci can facilitate the improvement of black point resistance in wheat. Here, 262 recombinant inbred lines (RILs) from the cross of Zhongmai 578/Jimai 22 were evaluated for their black point reactions in five environments. A high-density genetic linkage map of the RIL population was constructed with the wheat 50K single nucleotide polymorphism (SNP) array. Six stable QTLs for black point resistance were detected, QBp.caas-2A, QBp.caas-2B1, QBp.caas-2B2, QBp.caas-2D, QBp.caas-3A, and QBp.caas-5B, which explained 2.1–28.8% of the phenotypic variances. The resistance alleles of QBp.caas-2B1 and QBp.caas-2B2 were contributed by Zhongmai 578 while the others were from Jimai 22. QBp.caas-2B2, QBp.caas-2D and QBp.caas-3A overlapped with previously reported loci, whereas QBp.caas-2A, QBp.caas-2B1 and QBp.caas-5B are likely to be new. Five kompetitive allele-specific PCR (KASP) markers, Kasp_2A_BP, Kasp_2B1_BP, Kasp_2B2_BP, Kasp_3A_BP, and Kasp_5B_BP, were validated in a natural population of 165 cultivars. The findings of this study provide useful QTLs and molecular markers for the improvement of black point resistance in wheat through marker-assisted breeding.

Key words: black point , candidate gene , common wheat , Kompetitive allele-specific PCR , quantitative trait locus

Tiantian Chen, Lei Li, Dan Liu, Yubing Tian, Lingli Li, Jianqi Zeng, Awais Rasheed, Shuanghe Cao, Xianchun Xia, Zhonghu He, Jindong Liu, Yong Zhang. 利用全基因组连锁分析解析中麦578/济麦22 RIL群体黑胚病抗性遗传基础[J]. Journal of Integrative Agriculture, 2025, 24(9): 3311-3321.

Tiantian Chen, Lei Li, Dan Liu, Yubing Tian, Lingli Li, Jianqi Zeng, Awais Rasheed, Shuanghe Cao, Xianchun Xia, Zhonghu He, Jindong Liu, Yong Zhang. Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22[J]. Journal of Integrative Agriculture, 2025, 24(9): 3311-3321.

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利用全基因组连锁分析解析中麦578/济麦22 RIL群体黑胚病抗性遗传基础

Genome wide linkage mapping for black point resistance in a recombinant inbred line population of Zhongmai 578 and Jimai 22

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