鸡源阿尔伯蒂埃希氏菌中共携带blaCTX-M-55和blaTEM-141基因多重耐药质粒的鉴定及特性

doi:10.1016/j.jia.2023.12.038

摘要/Abstract

摘要： 抗生素的不合理使用导致细菌耐药性日趋严重，已成为全球严重的公共卫生问题。产超广谱β-内酰胺酶（ESBL）细菌因具有耐药性强、耐药谱广、散播快等特点，倍受关注。为了解肉鸡中阿尔伯蒂埃希氏菌的耐药特征及耐药质粒携带情况，本研究从养禽场采集50份病鸡脾脏和肝脏样品，通过选择性培养基、PCR方法分离鉴定阿尔伯蒂埃希氏菌。然后测定阿尔伯蒂埃希氏菌分离株的耐药性及耐药基因，并对多重耐药分离株进行接合试验、全基因组测序分析耐药基因的可转移性及耐药质粒特征。经过选择性培养基分离、PCR检测共鉴定4株阿尔伯蒂埃希氏菌。药物敏感性敏结果显示，4株分离株对四环素类、氯霉素类、β-内酰胺类、氨基糖苷类、多粘菌素B、磺胺类、喹诺酮类等7类抗生素具有耐药性。其中，EA04菌株携带ESBL基因bla_CTX-M和bla_TEM。基于ST4638型阿尔伯蒂埃希氏菌的核心基因组多位点序列分型（cgMLST）构建系统进化树，推断EA04菌株与人源阿尔伯蒂埃希氏菌分离株处于相近进化分支。全基因组测序表明，ESBL基因bla_CTX-M-55和bla_TEM-141共存于大小约为75 kb的IncFII型质粒pEA04.2。比较基因组分析表明，bla_CTX-M-55和bla_TEM-141基因位于IS15-bla_CTX-M-55-wbuC-bla_TEM-141-IS26区域，在其他多种细菌中发现高度同源序列。接合试验显示，质粒pEA04.2可转移至大肠杆菌EC600，并介导对第三代头孢菌素类抗生素产生耐药性。本研究分离到一株多重耐药阿尔伯蒂埃希氏菌，并首次揭示了阿尔伯蒂埃希氏菌携带的bla_CTX-M-55和bla_TEM-141基因位于可转移质粒pEA04.2的IS15-bla_CTX-M-55-wbuC-bla_TEM-141-IS26区域中，可导致其对第三代头孢菌素药物产生耐药性。研究结果表明，鸡可能会作为多重耐药阿尔伯蒂埃希氏菌的储藏库，从而增加了耐药基因在人类、动物和环境之间横向传播的风险，具有潜在的公共卫生学意义。

Abstract: The inappropriate use of cephalosporins lead to the occurrence and global spread of bacteria resistant to these antimicrobials. In this study, we isolated four Escherichia albertii (E. albertii) strains from broilers in Eastern China. The antimicrobial susceptibility and genomic characterization of these E. albertii isolates were determined. Our results revealed that these four E. albertii isolates exhibited resistance to tetracyclines, chloramphenicol, β-lactams, aminoglycosides, polymyxin B, sulfonamides, quinolones, and other antimicrobials. Among them, EA04 isolate was multidrug resistant and harbored extended-spectrum β-lactamases (ESBL) genes bla_CTX-M and bla_TEM. Whole genome sequencing and core-genome Multilocus sequence typing (cgMLST) based on all ST4638 E. albertii for EA04 inferred highly probable epidemiological links between selected human isolates. Additionally, the ESBL genes bla_TEM-141 and bla_CTX-M-55 were coexistent in an approximately 75 kb IncFII plasmid pEA04.2 in EA04. Comparative analysis indicated that genes bla_TEM-141 and bla_CTX-M-55 were located in IS15-bla_CTX-M-55-wbuC-bla_TEM-141-IS26 region, which similar structures were identified in various bacteria. Furthermore, the plasmid pEA04.2 could be transferable to Escherichia coli EC600 and lead to the resistance to third-generation cephalosporins. These results suggested that chicken potentially serve as a reservoir for multidrug resistant E. albertii, which increases the risk of horizontal transfer of antimicrobial resistance between humans, animals and environment.

Key words: Escherichia albertii , , antimicrobial resistance , , plasmid , , whole genome sequencing

. 鸡源阿尔伯蒂埃希氏菌中共携带bla_CTX-M-55和bla_TEM-141基因多重耐药质粒的鉴定及特性[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2023.12.038.

Weiqi Guo, Di Wang, Xinyu Wang, Zhiyang Wang, Hong Zhu, Jiangang Hu, Beibei Zhang, Jingjing Qi, Mingxing Tian, Yanqing Bao, Na Li, Wanjiang Zhang, Shao-hui Wang. Identification and characterization of a plasmid co-harboring bla_CTX-M-55and bla_TEM-141in Escherichia albertii from broiler in China[J]. Journal of Integrative Agriculture, DOI: 10.1016/j.jia.2023.12.038.

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