蒺藜苜蓿MtRAV1基因调节生长发育的功能分析

doi:10.1016/j.jia.2023.12.032

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (5): 1944-1957.DOI: 10.1016/j.jia.2023.12.032

蒺藜苜蓿MtRAV1基因调节生长发育的功能分析

收稿日期:2023-04-16 修回日期:2024-01-03 接受日期:2023-11-28 出版日期:2025-05-20 发布日期:2025-04-18

Functional identification of Medicago truncatula MtRAV1 in regulating growth and development

Shumin Wang^{1, 2*}, Tao Guo^3*, Shaolin Zhang², Hong Yang², Li Li², Qingchuan Yang¹, Junping Quan^2#, Ruicai Long^1#

¹Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China
²Nanshan Botanical Garden Management Office, Chongqing 400065, China
³Chongqing Key Laboratory of Germplasm Innovation and Utilization of Native Plants, Chongqing Landscape and Gardening Research Institute, Chongqing 401329, China

Received:2023-04-16 Revised:2024-01-03 Accepted:2023-11-28 Online:2025-05-20 Published:2025-04-18
About author:Shumin Wang, E-mail: wsmin2011@126.com; Tao Guo, E-mail: yushen0002008@126.com; #Correspondence Ruicai Long, E-mail: dragongodsgod@163.com; Junping Quan, E-mail: 151209328@qq.com * These authors contributed equally to this study.
Supported by:
This work was supported by the Key Projects in Science and Technology of Inner Mongolia, China (2021ZD0031), the China Agriculture Research System of MOF and MARA (CARS-34), the Performance Incentive and Guidance Special Project of Scientific Research Institution, Chongqing Science and Technology Committee, China (cstc2022jxjl80019), and the Natural Science Foundation of Chongqing, China (CSTB2022NSCQ-BHX0744).

摘要/Abstract

摘要：

RAV（Related to ABI3/VP1）转录因子属于AP2或B3超家族，是植物特异性转录因子。已有研究表明，RAVs基因参与植物生长发育调控和激素信号转导，但其具体作用机制还不清晰，有待于进一步研究。为研究苜蓿RAVs基因分子调控机制，本研究从蒺藜苜蓿中筛选到3个RAVs基因，对其进行进化分析，将其中一个RAV基因命名为MtRAV1，构建了该基因的过表达载体并转化蒺藜苜蓿，对过表达转基因株系与野生型植株进行了表型比较分析。为进一步筛选MtRAV1基因下游作用基因，使用DAP-seq和RNA-seq方法过表达转基因株系与野生型植株进行测序比较分析。研究结果表明，MtRAV1过表达蒺藜苜蓿与野生型相比表现出植株矮化、开花延迟、叶片和花器官变小、分枝数量增加、果荚和种子变小等性状，基因表达分析结果表明MtRAV1过表达株系中MtFTa1、MtSOC1、MtGA3OX1、MtDWARF14和MtCCD7等开花调控和生长调控相关基因的表达受到明显抑制。RNA-seq分析结果表明MtRAV1可能通过影响光合作用、昼夜节律和植物激素（特别是生长素）信号传导途径中相关基因的表达来调控植物的生长发育。DAP-seq与RNA-seq联合分析结果表明MtRAV1抑制光合系统II和生长素信号通路相关基因MtFd-l3、MtLhcb-l2和MtSAUR-l等的表达，进而抑制植物生长。综上所述，本研究解析了MtRAV1在蒺藜苜蓿生长发育调控过程中的作用机制，预测其主要通过生长素信号传导途径影响植物的生长发育，MtRAV1基因被初步证明为蒺藜苜蓿的一个关键生长抑制因子，该基因为高产早熟苜蓿新品种的选育提供了潜在的候选基因。

Abstract:

Related to ABI3 and VP1 (RAV) transcription factors belong to the AP2 and B3 superfamily. RAVs genes have been reported to be involved in plant growth and development regulation. This study screened three RAV genes from Medicago truncatula and named one of them MtRAV1. The MtRAV1 overexpressing plants exhibits traits such as plant dwarfing, delayed flowering, reduced leaf and floral organs, increased branching, and reduced pods and seeds. Gene expression analysis results showed that overexpression of MtRAV1 inhibited the expression of Flowering Locus T (MtFTa1), Suppressor of Overexpression of CO 1 (MtSOC1), GA3-oxidase1 (MtGA3OX1), DWARF14 (MtD14) and Carotenoid Cleavage Dioxygenase 7 (MtCCD7). To further investigate the regulation pathway involved by MtRAV1, RNA-sequencing (RNA-seq) and DNA affinity purification sequencing (DAP-seq) analysis were conducted. RNA-seq results indicated that MtRAV1 might affect plant growth and development by regulating some genes in photosynthesis, circadian rhythm and plant hormone signaling pathways, especially the auxin signaling pathway. Conjoint analysis of DAP-seq and RNA-seq revealed that MtRAV1 might inhibit the expression of Ferredoxin (MtFd-l3), Light-harvesting Chlorophyll a/b Binding Protein 1 (MtLhcb-l2) and Small Auxin Up-regulated RNA (MtSAUR-l), which related to photosystem II and auxin signaling pathway. Summarily, MtRAV1 was preliminarily proven to be a key growth inhibitory factor in M. truncatula.

Key words: RAV transcription factor , growth and development , photosynthesis , plant hormone signal transduction

Shumin Wang, Tao Guo, Shaolin Zhang, Hong Yang, Li Li, Qingchuan Yang, Junping Quan, Ruicai Long. 蒺藜苜蓿MtRAV1基因调节生长发育的功能分析[J]. Journal of Integrative Agriculture, 2025, 24(5): 1944-1957.

Shumin Wang, Tao Guo, Shaolin Zhang, Hong Yang, Li Li, Qingchuan Yang, Junping Quan, Ruicai Long. Functional identification of Medicago truncatula MtRAV1 in regulating growth and development[J]. Journal of Integrative Agriculture, 2025, 24(5): 1944-1957.

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蒺藜苜蓿MtRAV1基因调节生长发育的功能分析

Functional identification of Medicago truncatula MtRAV1 in regulating growth and development

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