Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (2): 731-734.DOI: 10.1016/j.jia.2023.11.042

• • 上一篇    

恶唑烷酮/苯丙醇耐药基因optrA在猪源多发性链球菌的全球首次发现

  

  • 收稿日期:2023-06-23 接受日期:2023-10-30 出版日期:2024-02-20 发布日期:2024-01-30

First identification of the oxazolidinone/phenicol resistance gene optrA in Streptococcus pluranimalium worldwide

Kuan Zhao1*, Longyu Zhou2, 3*, Shixia Zhang1, Wanjiang Zhang2#, Yao Zhu2#   

  1. 1 College of Veterinary Medicine, Hebei Agricultural University, Baoding 071001, China

    2 State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China

    3 College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China

  • Received:2023-06-23 Accepted:2023-10-30 Online:2024-02-20 Published:2024-01-30
  • About author:ZHAO Kuan, Fax: +98-0312-7528377, E-mail: zhaokuan519@126.com; ZHOU Long-yu, Tel: E-mail: zly6987@163.com; #Correspondence ZHANG Wan-jiang, Tel: +86-451-51051734, Fax: +86-0451-51997166, E-mail: zhangwanjiang@caas.cn; ZHU Yao, Tel: +86-451-51051734, Fax: +86-0451-51997166, E-mail: yaozhu922@163.com *These authors contributed equally to the work.
  • Supported by:
    This work was supported by the National Key Research and Development Program of China (grant no. 2022YFF0710505). The central Public-interest Scientific Institution Basal Research Fund (grant no. 1610302022001) and the Talents Introduction Projects of Hebei Agricultural University (grant no. YJ201945).

摘要:

恶唑烷酮类抗生素作为目前治疗多重耐药革兰氏阳性菌感染最有效的抗生素之一,其耐药基因optrA的流行和传播备受关注,然而,目前optrA基因在链球菌中的流行情况尚不十分清楚。本文从山东省某猪场分离出一株多发性链球菌(Streptococcus pluranimalium S. pluranimaliumSP28,通过肉汤稀释法测定了该分离株的药物敏感性,其次利用IlluminaNanopore测序技术测定了该分离株的全基因组序列,通过生物信息学技术对全基因组序列进行比对分析,最后利用PCR方法检测插入序列IS1216E介导的易位单元(Translocatable UnitTU)。研究结果显示,分离株SP28表现出多重耐药表型和相对高的利奈唑胺MIC值。全基因组测序结果表明,成功获得了该分离株的完整染色体序列和一个质粒序列。耐药基因检测分析发现,该分离株的染色体携带optrA基因以及多个介导其它类型抗生素的耐药基因。耐药基因环境分析显示optrAfexA所在的耐药区域侧翼为两个同向的IS1216E插入序列,PCR检测结果显示携带optrA的耐药区域能够利用TU的方式进行水平转移,进而导致optrA的广泛传播。本研究首次在S. pluranimalium中发现耐药基因optrA,同时进一步揭示了IS1216EoptrA基因转移中发挥的重要作用。

关键词: 恶唑烷酮类抗生素 ,  optrA基因 ,  多发性链球菌 ,  IS1216E

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