黄瓜黄色条纹转运蛋白基因启动子突变导致黄色子叶表型的研究

doi:10.1016/j.jia.2023.11.024

摘要/Abstract

摘要：

在高等植物中，叶色突变体被视为研究叶绿素生物合成、光合作用机制和叶绿体发育的理想材料。在此，我们鉴定了一个在栽培黄瓜中表现出黄色子叶的自发突变体yc412。该黄致死突变体表现出现异常的叶绿体超微结构、较低的光合能力和色素含量。通过BSA测序和精细定位，我们将黄色子叶（yc）位点缩小到3号染色体上的一个～96.8 kb区间。通过重测序技术和分子克隆，我们确定Csyc为候选基因，该基因编码一个黄条纹转运蛋白。Csyc启动子区域的T到C突变导致yc412的黄色子叶表型。与YZU027A（野生型）相比，Csyc在yc412子叶中的表达显著下调。通过病毒诱导基因沉默技术在黄瓜中沉默Csyc，导致叶片的叶绿素含量显著降低，叶片褪绿。此外，转录组分析表明，在yc412子叶中，与叶绿体相关基因和叶绿素生物合成相关基因显著下调。本研究结果为黄条纹转运蛋白在植物叶绿体发育和叶绿素合成中的分子功能提供了新的认识。

Abstract: Leaf color mutants in higher plants are considered to be ideal materials for studying the chlorophyll biosynthesis, photosynthesis mechanism and chloroplast development. Herein, we identified a spontaneous mutant, yc412, in cultivated cucumber that exhibited yellow cotyledons. The yellow-lethal mutant was diagnosed with an abnormal chloroplast ultrastructure, and reduced photosynthetic capacity and pigment content. Through bulked segregant analysis-based whole-genome sequencing and fine genetic mapping, we narrowed the yellow cotyledons (yc) locus to a 96.8 kb interval on chromosome 3. By resequencing and molecular cloning, we showed that Csyc is a potential candidate gene, which encodes a yellow stripe-like (YSL) transporter. The T to C mutation in the promoter region of Csyc caused the yellow cotyledon phenotype in yc412. Compared to YZU027A (WT), the expression of Csyc was significantly downregulated in the cotyledons of yc412. Silencing of Csyc in cucumber via virus-induced gene silencing resulted in chlorotic leaves, mainly by suppressing the chlorophyll content. Furthermore, a comparative transcriptome analysis revealed that chloroplast-related genes and chlorophyll biosynthesis genes were significantly downregulated in yc412 cotyledons. Our results provide new insights into the molecular function of the YSL transporter in plant chloroplast development and chlorophyll synthesis.

Key words: cucumber , yellow-lethal cotyledons , chloroplast development , yellow stripe-like transporter

. 黄瓜黄色条纹转运蛋白基因启动子突变导致黄色子叶表型的研究[J]. Journal of Integrative Agriculture, 2024, 23(3): 849-862.

Jiawei Pan, Jia Song, Rahat Sharif, Xuewen Xu, Shutong Li, Xuehao Chen.

A mutation in the promoter of the yellow stripe-like transporter gene in cucumber results in a yellow cotyledon phenotype [J]. Journal of Integrative Agriculture, 2024, 23(3): 849-862.

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