EPSPS通过打破棉花中木质素和类黄酮生物合成的平衡来调节细胞伸长

doi:10.1016/j.jia.2023.11.002

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (10): 3437-3456.DOI: 10.1016/j.jia.2023.11.002

EPSPS通过打破棉花中木质素和类黄酮生物合成的平衡来调节细胞伸长

收稿日期:2023-07-31 接受日期:2023-09-25 出版日期:2024-10-20 发布日期:2024-09-11

EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton

Qingdi Yan^{1, 2}, Wei Hu³, Chenxu Gao³, Lan Yang¹, Jiaxian Yang³, Renju Liu³, Masum Billah¹, Yongjun Lin², Ji Liu^1, ⁴, Pengfei Miao^{1, 4}, Zhaoen Yang^{1, 3#}, Fuguang Li^{1, 3#}, Wenqiang Qin^{1, 4#}

¹State Key Laboratory of Cotton Bio‑breeding and Integrated Utilization/Institute of Cotton Research, Chinese Academy of Agricultural Sciences, Anyang 455000, China
² National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China
³ State Key Laboratory of Cotton Bio-breeding and Integrated Utilization/Zhengzhou University, Zhengzhou 450066, China
⁴National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572025, China

Received:2023-07-31 Accepted:2023-09-25 Online:2024-10-20 Published:2024-09-11
About author:#Correspondence Zhaoen Yang, E-mail: yangzhaoen0925@126.com; Fuguang Li, E-mail: Aylifug@caas.cn; Wenqiang Qin, E-mail: qinwenqiang2005@163.com
Supported by:
This work was supported by funding from the Natural Science Foundation of Henan Province, China (232300421010), the Key Research and Development Project of Henan Province, China (231111110400), the Hainan Provincial Joint Project of Sanya Yazhou Bay Science and Technology City, China (320LH045), the Hainan Yazhou Bay Seed Laboratory, China (B21HJ0215), the Fundamental Research Funds of State Key Laboratory of Cotton Biology, China (2021CBE03), the Central Public-interest Scientific Institution Basal Research Fund, China (Y2023XK16), and the Innovation Program of the Chinese Academy of Agricultural Sciences (CAAS-ASTIP-IVFCAAS to F.G.L).

摘要/Abstract

摘要：

EPSPS是莽草酸合成途径中的关键基因，已被广泛用于培育抗除草剂作物。然而，它在调节细胞伸长中的作用却知之甚少。通过过表达EPSPS基因，我们产生了对草甘膦有抗性的品系，表现出意想不到的矮化表型。代表性品系DHR1在其整个生长期表现出稳定的矮化表型。除株高外，DHR1的其他农艺性状与其转基因外植体ZM24相似。石蜡切片实验表明，由于节间细胞的伸长和分裂减少，导致DHR1节间缩短。外源激素恢复实验证实DHR1不是典型的BR或GA相关的矮化突变体。杂交分析和精细定位证实了EPSPS基因是矮化的因果基因，并且该表型可以在不同的基因型中遗传。转录组和代谢组分析显示，与ZM24相比，苯丙烷合成途径相关的基因在DHR1中富集。类黄酮代谢物在DHR1中富集，而木质素代谢物减少。黄酮类化合物的增加可能导致生长素信号通路基因的差异表达，并改变生长素反应，从而影响细胞伸长。这项研究提供了一种新的矮化策略，并将加速棉花栽培的轻型化和机械化收获的改进。

Abstract:

EPSPS is a key gene in the shikimic acid synthesis pathway that has been widely used in breeding crops with herbicide resistance. However, its role in regulating cell elongation is poorly understood. Through the overexpression of EPSPS genes, we generated lines resistant to glyphosate that exhibit an unexpected dwarf phenotype. A representative line, DHR1, exhibits a stable dwarf phenotype throughout its entire growth period. Except for plant height, the other agronomic traits of DHR1 are similar to its transgenic explants ZM24. Paraffin section observations showed that DHR1 internodes are shortened due to reduced elongation and division of the internode cells. Exogenous hormones confirmed that DHR1 is not a classical brassinolide (BR)- or gibberellin (GA)-related dwarfing mutant. Hybridization analysis and fine mapping confirmed that the EPSPS gene is the causal gene for dwarfism, and the phenotype can be inherited in different genotypes. Transcriptome and metabolome analyses showed that genes associated with the phenylpropanoid synthesis pathway are enriched in DHR1 compared with ZM24. Flavonoid metabolites are enriched in DHR1, whereas lignin metabolites are reduced. The enhancement of flavonoids likely results in differential expression of auxin signal pathway genes and alters the auxin response, subsequently affecting cell elongation. This study provides a new strategy for generating dwarfs and will accelerate advancements in light simplification in the cultivation and mechanized harvesting of cotton.

Key words: EPSPS , cotton , T-DNA , dwarfism , herbicide resistance

Qingdi Yan, Wei Hu, Chenxu Gao, Lan Yang, Jiaxian Yang, Renju Liu, Masum Billah, Yongjun Lin, Ji Liu, Pengfei Miao, Zhaoen Yang, Fuguang Li, Wenqiang Qin. EPSPS通过打破棉花中木质素和类黄酮生物合成的平衡来调节细胞伸长[J]. Journal of Integrative Agriculture, 2024, 23(10): 3437-3456.

Qingdi Yan, Wei Hu, Chenxu Gao, Lan Yang, Jiaxian Yang, Renju Liu, Masum Billah, Yongjun Lin, Ji Liu, Pengfei Miao, Zhaoen Yang, Fuguang Li, Wenqiang Qin. EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton[J]. Journal of Integrative Agriculture, 2024, 23(10): 3437-3456.

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EPSPS通过打破棉花中木质素和类黄酮生物合成的平衡来调节细胞伸长

EPSPS regulates cell elongation by disrupting the balance of lignin and flavonoid biosynthesis in cotton

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