玉米雌穗发育及下位小花退化相关基因MIR172e的精细定位与功能分析

doi:10.1016/j.jia.2023.10.030

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (4): 1372-1389.DOI: 10.1016/j.jia.2023.10.030

玉米雌穗发育及下位小花退化相关基因MIR172e的精细定位与功能分析

收稿日期:2023-07-10 接受日期:2023-10-08 出版日期:2025-04-20 发布日期:2025-03-17

Fine mapping and discovery of MIR172e, a candidate gene required for inflorescence development and lower floret abortion in maize ear

Lanjie Zheng^1*, Qianlong Zhang^1*, Huiying Liu¹, Xiaoqing Wang³, Xiangge Zhang⁴, Zhiwei Hu², Shi Li¹, Li Ji¹, Manchun Ji¹, Yong Gu⁵, Jiaheng Yang¹, Yong Shi¹, Yubi Huang^2#, Xu Zheng^1#

¹State Key Laboratory of Wheat and Maize Crop Science/Center for Crop Genome Engineering, College of Agronomy, Henan Agricultural University, Zhengzhou 450046, China
²College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China
³School of Agricultural Sciences, Zhengzhou University, Zhengzhou 450002, China
⁴Industrial Crop Research Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China
⁵Sichuan Yuliang Testing Co., Ltd., Chengdu 611130, China

Received:2023-07-10 Accepted:2023-10-08 Online:2025-04-20 Published:2025-03-17
About author:Lanjie Zheng, E-mail: zhenglanjie@henau.edu.cn; Qianlong Zhang, E-mail: 13525484695@163.com; #Correspondence Xu Zheng, Tel: +86-371-56990188, E-mail: zhengxu@henau.edu.cn; Yubi Huang, +86-28-86290875, E-mail: yubihuang@sohu.com * These authors contributed equally to this study.
Supported by:
This work was supported by the Natural Science Foundation of Henan Province, China (232300421260), the Tackling Key Problems in Science and Technology of Henan Province, China (222102110465, to LZ and 232102111097, to YS), and the Open Project Program (SKL-KF202214) of the State Key Laboratory of Crop Gene Exploration and Utilization in Southwest China.

摘要/Abstract

摘要：

玉米（Zea mays L.）是雌雄同株异花植物，其雌穗和雄穗分别为雌花序和雄花序。成熟的玉米雌花序通常能产生数百粒籽粒，决定籽粒的大小及粒重，直接影响玉米的最终产量。本研究鉴定了一个玉米隐性花序发育缺陷突变体tasselseed2016（ts2016），该突变体雌雄花序在发育过程中表现出多方面的缺陷且籽粒产量降低，包括雌雄花序上各类分生组织的确定性和小花中器官身份的缺失、雌穗下位小花退化障碍及籽粒变小。通过图位克隆及等位测试，确定该表型由microRNA基因MIR172e基因突变造成。此外，本研究鉴定到一个调控玉米雌穗下位小花退化的分子模块——miR172e/ETHYLENE RESPONSIVE ELEMENT BINDING197 (EREB197)。转录组分析表明，MIR172e突变能抑制玉米雌穗发育中的多个生物过程，特别是花发育和激素相关途径。另外，我们发现MIR172e DNA序列突变影响其RNA的转录，导致突变位点后的转录延伸受阻。本研究揭示了MIR172e在玉米花序发育和籽粒产量中的作用和分子机制，加深了我们对玉米花发育调控的认识。

Abstract:

Maize (Zea mays L.) is a monoecious grass species with separate male and female inflorescences which form the tassel and ear, respectively. The mature ear inflorescences usually bear hundreds of grains, so they directly influence maize grain production and yield. Here, we isolated a recessive maize mutant, tasselseed2016 (ts2016), which exhibits pleiotropic inflorescence defects and reduced grain yield. These defects include the loss of determinacy and identity in meristems and floral organs, as well as a lack of the lower floret abortion in maize ear, and a smaller grain size. Using map-based cloning and allelic testing, we identified and confirmed the microRNA gene MIR172e as the target gene controlling these related traits. Furthermore, our evidence uncovered a new potential miR172e/ETHYLENE RESPONSIVE ELEMENT BINDING197 (EREB197) regulatory module which controls lower floret abortion in maize ear. Transcriptome analysis revealed that the mutation of MIR172e represses multiple biological processes, particularly the flower development and hormone-related pathways in maize ear. We also found that a mutation in the DNA sequence of MIR172e affects RNA transcription, resulting in elongation blockage at the mutant site. Our results reveal the function and molecular mechanism of MIR172e in maize inflorescences and grain yield, and this study deepens our knowledge of maize inflorescence development.

Key words: inflorescence , ear , ts2016 , MIR172e , grain yield , maize (Zea mays L.)

Lanjie Zheng, Qianlong Zhang, Huiying Liu, Xiaoqing Wang, Xiangge Zhang, Zhiwei Hu, Shi Li, Li Ji, Manchun Ji, Yong Gu, Jiaheng Yang, Yong Shi, Yubi Huang, Xu Zheng. 玉米雌穗发育及下位小花退化相关基因MIR172e的精细定位与功能分析[J]. Journal of Integrative Agriculture, 2025, 24(4): 1372-1389.

Lanjie Zheng, Qianlong Zhang, Huiying Liu, Xiaoqing Wang, Xiangge Zhang, Zhiwei Hu, Shi Li, Li Ji, Manchun Ji, Yong Gu, Jiaheng Yang, Yong Shi, Yubi Huang, Xu Zheng. Fine mapping and discovery of MIR172e, a candidate gene required for inflorescence development and lower floret abortion in maize ear[J]. Journal of Integrative Agriculture, 2025, 24(4): 1372-1389.

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玉米雌穗发育及下位小花退化相关基因MIR172e的精细定位与功能分析

Fine mapping and discovery of MIR172e, a candidate gene required for inflorescence development and lower floret abortion in maize ear

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