中国H10Nx亚型禽流感病毒的遗传进化及生物学特性分析

doi:10.1016/j.jia.2023.10.028

摘要/Abstract

摘要：

H10亚型禽流感病毒（AIV）在中国流行的40余年间感染宿主从野生鸟类扩大到家禽和哺乳动物。近年，H10亚型AIV在中国南方多省造成人感染事件。辽宁省位于中国东北部，该省以前少有H10亚型禽流感病毒的相关研究。本研究在AIV主动监测工作中，自辽宁省活禽市场分离到一株H10N3型禽流感病毒，命名为A/chicken/Liaoning/SY1080/2021(SY1080)。SY1080可在小鼠肺脏和鼻甲内复制，对小鼠呈低致病性。本研究将SY1080与国内已公布的256株H10亚型AIV一同进行遗传进化、宿主多样性、分子特征等分析，系统解析H10Nx亚型AIV在中国的遗传进化规律。进化分析表明，包括SY1080毒株在内的250株H10亚型AIV属于欧亚谱系，只有7株H10亚型AIV属于北美谱系。我国分离的H10亚型AIV可划分为63种基因型，SY1080与H10N3亚型AIV人源分离株，均属于G60基因型。H10亚型AIV在流行过程中获得多个哺乳动物适应性和毒力增强的相关位点突变，并且与H9N2亚型AIV重组，产生内部基因以H9N2亚型AIV为主的H10亚型重组病毒。本研究总结了H10Nx病毒在我国遗传进化规律和主要生物学特性，研究结果提示该亚型病毒具有潜在公共卫生风险，应加强对H10亚型毒株的监测。

Abstract:

H10 subtype avian influenza viruses (AIV) have been circulating in China for 40 years. H10 AIVs in China have expanded their host range from wild birds to domestic poultry and mammals, even human. Most of the H10 subtype AIVs reported in China were isolate from the southeast part. We isolated an H10N3 AIV, A/Chicken/Liaoning/SY1080/2021 (SY1080), from live poultry market (LPM) in Liaoning Province of the Northeast China. SY1080 replicated efficiently in mice lungs and nasal turbinates without prior adaptation. We systematically compared SY1080 with other H10 subtype isolates in China. Phylogenetic analysis showed that SY1080 and most of the H10 strains belonged to the Eurasian lineage. H10 AIVs in China have formed 63 genotypes. SY1080 as well as the H10N3 strains from human infections belonged to G60 genotype. H10Nx AIV acquired multiple mammalian adaptive and virulence related mutations during circulation and the recent reassortants derived internal genes from chicken H9N2 AIVs. The H10Nx subtypes AIVs posed potential threat to public health. These results suggested we should strengthen the surveillance and evaluation of H10 subtype strains.

Key words: avian influenza virus , H10 subtype , adaptation , pathogenicity

Yina Xu, Hailing Li, Haoyu Leng, Chaofan Su, Siqi Tang, Yongtao Wang, Shiwei Zhang, Yali Feng, Yanan Wu, Daxin Wang, Ying Zhang. 中国H10Nx亚型禽流感病毒的遗传进化及生物学特性分析[J]. Journal of Integrative Agriculture, 2024, 23(11): 3860-3869.

Yina Xu, Hailing Li, Haoyu Leng, Chaofan Su, Siqi Tang, Yongtao Wang, Shiwei Zhang, Yali Feng, Yanan Wu, Daxin Wang, Ying Zhang. Genetic and biological properties of H10Nx influenza viruses in China[J]. Journal of Integrative Agriculture, 2024, 23(11): 3860-3869.

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