ABA诱导基因IbTSJT1正调控转基因甘薯的抗旱性

doi:10.1016/j.jia.2023.10.015

摘要/Abstract

摘要：

TSJT1属于 Class-Ⅱ谷氨酰胺酰胺基转移酶超家族成员。目前有关TSJT1在植物中的作用及调控机制的研究很少。本研究从甘薯抗旱品系徐薯55-2中分离出受植物激素ABA诱导的基因IbTSJT1。该基因的表达受PEG6000和ABA的强烈诱导。亚细胞定位表明，IbTSJT1蛋白定位于细胞核和细胞膜上。过表达IbTSJT1基因显著提高了甘薯的抗旱性。干旱条件下，过表达甘薯植株中ABA和脯氨酸含量提高，SOD和POD活性增强，ROS清除系统基因被上调。叶片气孔开度实验表明，过表达IbTSJT1基因提高了转基因甘薯植株的ABA敏感性。酵母单杂交（Y1H）、凝胶阻滞实验（EMSA）、双荧光素酶（Dual-Luc）和染色质免疫沉淀（ChIP-qPCR）等实验结果表明，IbABF2直接结合在IbTSJT1基因启动子中的ABA响应元件ABRE上，并激活其表达。这些研究结果表明，IbTSJT1基因介导ABA依赖的干旱胁迫响应，并通过诱导气孔关闭和激活ROS清除系统来增强转基因甘薯的抗旱性。本研究为提高甘薯和其他植物的抗旱性提供了新的候选基因。

Abstract:

The TSJT1 protein belongs to the class-II glutamine amidotransferase (GATase) superfamily. Research on the functions and underlying mechanisms of TSJT1 in plants is limited. In this study, the abscisic acid (ABA)-inducible gene IbTSJT1 was isolated from drought-tolerant sweetpotato line Xushu 55-2. Its expression was strongly induced by PEG6000 and ABA. The IbTSJT1 protein was localized in the nucleus and cell membrane. IbTSJT1-overexpressing sweetpotato plants exhibited significantly enhanced drought tolerance. Their ABA and proline contents and superoxide dismutase (SOD) and peroxidase (POD) activities were increased, and their reactive oxygen species (ROS) scavenging-related genes were upregulated under drought stress. The stomatal aperture assay confirmed that the IbTSJT1-overexpressing plants had greater sensitivity to ABA. The results of yeast one-hybrid (Y1H) assay, electrophoretic mobility shift assay (EMSA), luciferase reporter assay and ChIP-qPCR assay indicated that IbABF2 can directly bind to the cis-acting ABA-responsive element (ABRE) in the IbTSJT1 promoter to activate the expression of IbTSJT1. These findings suggest that IbTSJT1 mediates ABA-dependent drought stress responses and enhances drought tolerance by inducing stomatal closure and activating the ROS scavenging system in transgenic sweetpotato. Our study provides a novel gene for improving drought tolerance in sweetpotato and other plants.

Key words: sweetpotato , IbTSJT1 , ABA , IbABF2 , drought tolerance

Yuxin Wang, Huan Zhang, Shaopei Gao, Hong Zhai, Shaozhen He, Ning Zhao, Qingchang Liu. ABA诱导基因IbTSJT1正调控转基因甘薯的抗旱性[J]. Journal of Integrative Agriculture, 2025, 24(4): 1390-1402.

Yuxin Wang, Huan Zhang, Shaopei Gao, Hong Zhai, Shaozhen He, Ning Zhao, Qingchang Liu. The ABA-inducible gene IbTSJT1 positively regulates drought tolerance in transgenic sweetpotato[J]. Journal of Integrative Agriculture, 2025, 24(4): 1390-1402.

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