蓝光通过调节橙色大白菜內叶中的类胡萝卜素代谢物诱导叶片颜色变化

doi:10.1016/j.jia.2023.09.029

摘要/Abstract

摘要：

类胡萝卜素参与植物叶色的形成以及光系统的光保护。在这项研究中，我们发现蓝光会显着上调橙色大白菜内叶中的总类胡萝卜素。此外，转录组学分析显示，蓝光处理诱导类胡萝卜素代谢途径上游的光合作用（BrHY5-2、BrCOP1、BrDET1）和MEP（甲基赤藓糖醇 4-磷酸）（BrGGPS、BrDXS、BrHDR）途径中的基因上调。类胡萝卜素代谢组学分析显示，蓝光处理后几种橙色和红色类胡萝卜素（番茄红素、玉米黄质、β-胡萝卜素、叶黄素和β-隐黄质）的积累有助于叶片橙色的加深，表明短期蓝光治疗可用于促进“有益营养”的积累。光信号基因 BrHY5-2 参与了橙色大白菜中类胡萝卜素生物合成的蓝光诱导转录调控。BrHY5-2在拟南芥中的过表达显著增加了总类胡萝卜素含量和对蓝光的敏感性。上述发现揭示了蓝光诱导橙色大白菜品系中类胡萝卜素合成和积累的新见解，并提供了提高蔬菜营养价值的新方法。

Abstract:

Carotenoids are involved in the formation of plant leaf color as well as photosystem photoprotection. This study showed that blue light significantly induced up-regulation of the total carotenoid content in the inner leaves of orange-head Chinese cabbage (OHCC). Furthermore, the transcriptomic analysis revealed that blue light treatment induced up-regulation of genes in photosynthesis (BrHY5-2, BrCOP1 and BrDET1) and the methylerythritol 4-phosphate pathways (BrGGPS, BrDXS and BrHDR) upstream of the carotenoid metabolic pathway. Carotenoid metabolomic analysis revealed that the accumulation of several orange and red carotenoids (lycopene, zeaxanthin, β-carotene, lutein, and β-cryptoxanthin) after blue light treatment contributed to the deepening of the leaf coloration, suggesting that short-term blue light treatment could be used to boost nutritional quality. The light signal gene BrHY5-2 participated in the blue light-induced transcriptional regulation of carotenoid biosynthesis in OHCC. Overexpression of BrHY5-2 in Arabidopsis significantly increased the total carotenoid content and the sensitivity to blue light. The above findings revealed new insights about blue-light-induced carotenoid synthesis and accumulation in OHCC lines. They suggested a new engineering approach to increase the nutritional value of vegetables.

Key words: orange heading Chinese cabbage (OHCC) , carotenoid , nutrition , blue LED light , secondary metabolite , transcriptome

ZHANG Rui-xing, ZHANG Ni-nan, WANG Ya-xiu, Khan ABID, MA Shuai, BAI Xue, ZENG Qi, PAN Qi-ming, LI Bao-hua, ZHANG Lu-gang. 蓝光通过调节橙色大白菜內叶中的类胡萝卜素代谢物诱导叶片颜色变化[J]. Journal of Integrative Agriculture, 2023, 22(11): 3296-3311.

ZHANG Rui-xing, ZHANG Ni-nan, WANG Ya-xiu, Khan ABID, MA Shuai, BAI Xue, ZENG Qi, PAN Qi-ming, LI Bao-hua, ZHANG Lu-gang. Blue light induces leaf color change by modulating carotenoid metabolites in orange-head Chinese cabbage (Brassica rapa L. ssp. pekinensis)[J]. Journal of Integrative Agriculture, 2023, 22(11): 3296-3311.

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