草地贪夜蛾瞬时受体电位通道基因的鉴定及TRPA1的功能研究

doi:10.1016/j.jia.2023.09.023

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (6): 1994-2005.DOI: 10.1016/j.jia.2023.09.023

草地贪夜蛾瞬时受体电位通道基因的鉴定及TRPA1的功能研究

收稿日期:2023-04-27 接受日期:2023-08-02 出版日期:2024-06-20 发布日期:2024-05-30

Identification of transient receptor potential channel genes and functional characterization of TRPA1 in Spodoptera frugiperda

Yutong Zhang1*, Hangwei Liu2*, Song Cao2, 3*, Bin Li1, 2, Yang Liu1, Guirong Wang1, 2#

¹State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
²Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen 518120, China
³Institute of Evolution and Ecology, School of Life Sciences, Central China Normal University, Wuhan 430079, China

Received:2023-04-27 Accepted:2023-08-02 Online:2024-06-20 Published:2024-05-30
About author:Yutong Zhang, E-mail: 82101222333@caas.cn; Hangwei Liu, E-mail: liuhangwei2014@163.com; Song Cao, E-mail: csong@ccnu.edu.cn; #Correspondence Guirong Wang, E-mail: wangguirong@caas.cn * These authors contributed equally to this study.
Supported by:
This work was funded by the Shenzhen Science and Technology Program, China (KQTD20180411143628272), the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District, China (pt202101-02) and the National Key R&D Program of China (2022YFE0116500).

摘要/Abstract

摘要：

草地贪夜蛾是一种极具破坏性的害虫，由于其强大的繁殖和迁飞能力，已成为一种全球性害虫。瞬时受体电位（Transient Receptor Potential，TRP）通道是一个庞大的阳离子通道家族，在昆虫感知外界环境和维持内部稳态中发挥着举足轻重的作用。近年来，TRP通道在昆虫行为调控中的重要作用得到了广泛的研究。本研究从草地贪夜蛾的基因组中鉴定了15个TRP基因座共编码26个转录本，并分析了其在不同发育阶段的表达谱。结果表明，草地贪夜蛾含有4个TRPC基因、6个TRPA基因、1个TRPM基因、2个TRPV基因、1个TRPN基因和1个TRPML基因，但并未鉴定到TRPP基因。此外，利用爪蟾卵母细胞表达系统对SfruTRPA1的功能进行了鉴定，SfruTRPA1能被20 ~ 45℃范围内的升高温度激活，而在相同温度范围内重复刺激后无明显脱敏现象。另外，SfruTRPA1能被天然化学物质异硫氰酸烯丙酯（allyl isothiocyanate, AITC）和肉桂醛（cinnamaldehyde, CA）激活。本研究对草地贪夜蛾的TRP通道基因进行了鉴定，为开发针对TRP通道的新型农药提供了靶标，也为全面解析TRP通道在昆虫生理功能中的作用奠定了基础。

Abstract:

Spodoptera frugiperda is a highly destructive pest that has become a global problem due to its robust reproductive and migratory capabilities. Transient receptor potential (TRP) channels, which constitute a vast ion channel family, play pivotal roles in sensing the external environment and maintaining internal homeostasis in insects. TRP channels have been widely investigated for their critical roles in regulating various insect behaviors in recent years. In this study, we identified 15 TRP gene loci encoding 26 transcripts in the genome of S. frugiperda and analyzed their expression profiles at different developmental stages. The results revealed that S. frugiperda possesses four TRPC genes, six TRPA genes, one TRPM gene, two TRPV genes, one TRPN gene, and one TRPML gene, while a canonical TRPP is absent. Moreover, the SfruTRPA1 was functionally characterized using the Xenopus oocyte expression system. The results showed that SfruTRPA1 is activated by temperature increases from 20 to 45°C, and there is no significant desensitization after repeated stimuli within the same temperature range. Additionally, SfruTRPA1 is activated by certain natural chemicals, including allyl isothiocyanate (AITC) and cinnamaldehyde (CA). These findings provide valuable insights to the TRP genes in S. frugiperda.

Key words: Spodoptera frugiperda , transient receptor potential channel , expression profile , TRPA1 , Xenopus oocyte

. 草地贪夜蛾瞬时受体电位通道基因的鉴定及TRPA1的功能研究[J]. Journal of Integrative Agriculture, 2024, 23(6): 1994-2005.

Yutong Zhang, Hangwei Liu, Song Cao, Bin Li, Yang Liu, Guirong Wang.

Identification of transient receptor potential channel genes and functional characterization of TRPA1 in Spodoptera frugiperda [J]. Journal of Integrative Agriculture, 2024, 23(6): 1994-2005.

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草地贪夜蛾瞬时受体电位通道基因的鉴定及TRPA1的功能研究

Identification of transient receptor potential channel genes and functional characterization of TRPA1 in Spodoptera frugiperda

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