Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (5): 1685-1702.DOI: 10.1016/j.jia.2023.09.002

• • 上一篇    下一篇

大豆依赖根际酸性磷酸酶活性及特定磷矿化细菌参与酸性土壤有机磷循环

  

  • 收稿日期:2023-06-20 接受日期:2023-07-28 出版日期:2024-05-20 发布日期:2024-04-24

Soybean (Glycine max) rhizosphere organic phosphorus recycling relies on acid phosphatase activity and specific phosphorus-mineralizing-related bacteria in phosphate deficient acidic soils

Qianqian Chen1*, Qian Zhao1*, Baoxing Xie1*, Xing Lu1, Qi Guo1, Guoxuan Liu1, Ming Zhou1, Jihui Tian2, Weiguo Lu3, Kang Chen1, Jiang Tian1#, Cuiyue Liang1#   

  1. 1 Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

    2 College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China

    3 Henan Academy of Crops Molecular Breeding, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China

  • Received:2023-06-20 Accepted:2023-07-28 Online:2024-05-20 Published:2024-04-24
  • About author:#Correspondence Jiang Tian, Tel: +86-20-85283380, E-mail: jtian@scau.edu.cn; Cuiyue Liang, Mobile: +86-13570467247, E-mail: liangcy@scau.edu.cn * These authors contributed equally to this study.
  • Supported by:
    This work was supported by grants from the National Key Research and Development Program of China (2021YFF1000500), the Open Competition Program of Ten Major Directions of Agricultural Science and Technology Innovation for the 14th Five-Year Plan of Guangdong Province, China (2022SDZG07), the Key Areas Research and Development Programs of Guangdong Province, China (2022B0202060005), the STIC Grant of China (SGDX20210823103535007), the Major Program of Guangdong Basic and Applied Research, China (2019B030302006), and the Natural Science Foundation of Guangdong Province, China (2021A1515010826 and 2020A1515110261).

PDF

摘要：

细菌在调节土壤磷循环过程中发挥着重要的作用。而作物与磷有效性的交互作用对土壤细菌群落的影响，以及细菌群落的重塑对土壤磷循环的反馈作用尚不清楚。本研究选用6份磷效率不同的大豆（Glycine max）基因型为试验材料，进行不同磷肥水平处理的酸性土壤田间试验。测定了非根际土和根际土壤中酸性磷酸酶（Acid phosphatase，AcP）活性和有机磷浓度，并通过微生物高通量测序技术分析土壤16S rRNA和phoC基因细菌群落结构。试验结果表明，低磷肥处理条件下，土壤有机磷浓度与土壤AcP活性以及大豆植株磷含量均呈显著负相关。土壤磷有效性以及大豆根际效应均影响了土壤细菌群落组成。而根际优势物种变形菌门（Proteobacteria）在低磷处理下的相对丰度与土壤有机磷浓度和AcP活性密切相关。土壤phoC基因高通量测序结果显示，大豆根际土壤中贪铜菌属（Cupriavidus）和克雷伯氏菌（Klebsiella）的相对丰度较非根际土高，而黄单胞菌属（Xanthomonas）的相对丰度较低。其中，贪铜菌属（Cupriavidus）为土壤phoC基因优势细菌属，且与土壤有机磷浓度呈显著负相关。以上结果表明，在磷有效性较低的酸性土壤，大豆可能依赖其根系招募的phoC基因细菌（例如，贪铜菌属）所产生的酸性磷酸酶，活化利用土壤有机磷。

Abstract:

Bacteria play critical roles in regulating soil phosphorus (P) cycling.  The effects of interactions between crops and soil P-availability on bacterial communities and the feedback regulation of soil P cycling by the bacterial community modifications are poorly understood.  Here, six soybean (Glycine max) genotypes with differences in P efficiency were cultivated in acidic soils with long-term sufficient or deficient P-fertilizer treatments.  The acid phosphatase (AcP) activities, organic-P concentrations and associated bacterial community compositions were determined in bulk and rhizosphere soils.  The results showed that both soybean plant P content and the soil AcP activity were negatively correlated with soil organic-P concentration in P-deficient acidic soils.  Soil P-availability affected the ɑ-diversity of bacteria in both bulk and rhizosphere soils.  However, soybean had a stronger effect on the bacterial community composition, as reflected by the similar biomarker bacteria in the rhizosphere soils in both P-treatments.  The relative abundance of biomarker bacteria Proteobacteria was strongly correlated with soil organic-P concentration and AcP activity in low-P treatments.  Further high-throughput sequencing of the phoC gene revealed an obvious shift in Proteobacteria groups between bulk soils and rhizosphere soils, which was emphasized by the higher relative abundances of Cupriavidus and Klebsiella, and lower relative abundance of Xanthomonas in rhizosphere soils.  Among them, Cupriavidus was the dominant phoC bacterial genus, and it was negatively correlated with the soil organic-P concentration.  These findings suggest that soybean growth relies on organic-P mineralization in P-deficient acidic soils, which might be partially achieved by recruiting specific phoC-harboring bacteria, such as Cupriavidus.

Key words: organic phosphorus ,  acid phosphatase ,  soybean ,  bacterial community ,  phoC-harboring bacteria ,  rhizosphere