小粒水稻不育系卓201S粒型QTL遗传分析与精细定位

doi:10.1016/j.jia.2023.07.026

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (7): 2155-2163.DOI: 10.1016/j.jia.2023.07.026

小粒水稻不育系卓201S粒型QTL遗传分析与精细定位

收稿日期:2023-03-05 接受日期:2023-07-06 出版日期:2024-07-20 发布日期:2024-07-01

Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S

Bin Lei^{1, 2*}, Jiale Shao^2*, Feng Zhang², Jian Wang², Yunhua Xiao¹, Zhijun Cheng^2#, Wenbang Tang^{1, 4#}, Jianmin Wan^{2, 3#}

¹ College of Agronomy, Hunan Agricultural University, Changsha 410128, China
²National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
³State Key Laboratory for Crop Genetics and Germplasm Enhancement/Jiangsu Plant Gene Engineering Research Center, Nanjing Agricultural University, Nanjing 210095, China
⁴ State Key Laboratory of Hybrid Rice, Hunan Hybrid Rice Research Center, Changsha 410125, China

Received:2023-03-05 Accepted:2023-07-06 Online:2024-07-20 Published:2024-07-01
About author:#Correspondence Zhijun Cheng, E-mail: chengzhijun@caas.cn; Wenbang Tang, E-mail: tangwenbang@163.com; Jianmin Wan, E-mail: wanjianmin@caas.cn * These authors contributed equally to this study.
Supported by:
This research was supported by the National Natural Science Foundation of China (32172078 and U22A20502).

摘要/Abstract

摘要： 小粒水稻不育系卓201S (Z201S) 的开发和应用显示了其在机械化杂交水稻制种方面的潜力，从而显著降低制种成本。然而，调控卓201S小粒特性的分子机制尚不清楚。本研究利用小粒水稻不育系卓201S和普通粒型水稻品种R2115构建的近等基因系进行了遗传分析。结果表明，Z201S的小粒性状是由一个部分显性基因控制，同时提高了穗粒数。利用图位克隆的方法，将目标基因定位在2号染色体短臂、分子标记LB354和LB63之间约113 kb区间内。转基因分析和基因表达分析显示LOC_Os02g14760是最可能的候选基因，表明Z201S的小粒性状由一个新的基因位点控制。

Abstract: The development and application of the small-grain rice sterile line Zhuo201S (Z201S) has demonstrated its potential for mechanized hybrid rice seed production, leading to significant cost reductions. However, the molecular mechanism responsible for the small-grain size characteristic of Z201S remains unclear. In this study, we conducted a genetic analysis using near-isogenic lines constructed from Z210S, a small-grain rice sterile line, and R2115, a normal-grain variety. The results revealed that the small-grain trait in Z201S is governed by a single partially dominant gene which also enhances grain number. Through mapping, we localized the causal gene to the short arm of chromosome 2, within a 113 kb physical region delimited by the molecular markers S2-4-1 and LB63. Transgenic analysis and gene expression assays indicated LOC_Os02g14760 as the most likely candidate gene, suggesting that the small-grain size trait of Z201S is controlled by a novel locus that has not been previously identified.

Key words: rice , grain size , map-based cloning

Bin Lei, Jiale Shao, Feng Zhang, Jian Wang, Yunhua Xiao, Zhijun Cheng, Wenbang Tang, Jianmin Wan. 小粒水稻不育系卓201S粒型QTL遗传分析与精细定位[J]. Journal of Integrative Agriculture, 2024, 23(7): 2155-2163.

Bin Lei, Jiale Shao, Feng Zhang, Jian Wang, Yunhua Xiao, Zhijun Cheng, Wenbang Tang, Jianmin Wan. Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S[J]. Journal of Integrative Agriculture, 2024, 23(7): 2155-2163.

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小粒水稻不育系卓201S粒型QTL遗传分析与精细定位

Genetic analysis and fine mapping of a grain size QTL in the small-grain sterile rice line Zhuo201S

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