水稻生长素上调小RNA基因OsSAUR33促进种子耐老化

doi:10.1016/j.jia.2023.07.024

Journal of Integrative Agriculture ›› 2025, Vol. 24 ›› Issue (1): 61-71.DOI: 10.1016/j.jia.2023.07.024

水稻生长素上调小RNA基因OsSAUR33促进种子耐老化

收稿日期:2023-05-10 接受日期:2023-06-27 出版日期:2025-01-20 发布日期:2025-01-06

Small auxin-up RNA gene OsSAUR33 promotes seed aging tolerance in rice

Shan Sun^1*, Wenjun Li^2*, Yanfen Fang¹, Qianqian Huang¹, Zhibo Huang¹, Chengjing Wang¹, Jia Zhao¹,Yongqi He^1#, Zhoufei Wang^1#

¹Laboratory of Seed Science and Technology,South China Agricultural University/Guangdong Key Laboratory of Plant Molecular Breeding/State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China
²School of Agriculture, Yunnan University, Kunming 650091, China

Received:2023-05-10 Accepted:2023-06-27 Online:2025-01-20 Published:2025-01-06
About author:Shan Sun, E-mail: ss2021@stu.scau.edu.cn; Wenjun Li, E-mail: wenjunli@ynu.edu.cn; #Correspondence Zhoufei Wang, Tel: +86-20-85280203, E-mail: wangzf@scau.edu.cn; Yongqi He, Tel: +86-20-85280203, E-mail: hyq@scau.edu.cn *These authors contributed equally to this study.
Supported by:
This work was supported by the Key-Area Research and Development Program of Guangdong Province, China (2022B0202060006), the National Natural Science Foundation of China (32201838, 32272157, 32172052, and 31971995), the Natural Science Foundation of Guangdong Province, China (2023A1515012052), the Project of Sanya Yazhou Bay Science and Technology City, China (SCKJ-JYRC-2022-87), and the Double First-class Discipline Promotion Project, China (2021B10564001).

摘要/Abstract

摘要：

种子贮藏过程中的耐老化特性是作物生产的一个重要性状。然而，有关水稻生长素上调小RNA基因对种子耐老化能力的调控作用知之甚少。本研究报道了一个水稻生长素上调小RNA基因OsSAUR33参与调控种子耐老化能力。在种子萌发阶段，与未老化的种子相比，在老化种子中OsSAUR33表达被显著诱导。相应的，与野生型（WT）相比，在自然老化和人工老化条件下，OsSAUR33突变体种子活力显著下降。OsSAUR33基因通过增加种子发芽过程中活性氧（ROS）水平，从而提高老化种子的活力。在种子发芽过程中，与野生型相比，在OsSAUR33突变体的老化种子中ROS积累显著延迟。此外，过氧化氢（H₂O₂）处理能显著提高不同水稻品种老化种子的活力。总之，本研究结果为今后改善水稻种子耐老化特性提供重要理论和技术支撑。

Abstract:

Seed aging tolerance during storage is generally an important trait for crop production, yet the role of small auxin-up RNA genes in conferring seed aging tolerance is largely unknown in rice. In this study, one small auxin-up RNA gene, OsSAUR33, was found to be involved in the regulation of seed aging tolerance in rice. The expression of OsSAUR33 was significantly induced in aged seeds compared with unaged seeds during the seed germination phase. Accordingly, the disruption of OsSAUR33 significantly reduced seed vigor compared to the wild type (WT) in response to natural storage or artificial aging treatments. The rice OsSAUR33 gene promotes the vigor of aged seeds by enhancing their reactive oxygen species (ROS) level during seed germination, and the accumulation of ROS was significantly delayed in the aged seeds of Ossaur33 mutants in comparison with WT during seed germination. Hydrogen peroxide (H₂O₂) treatments promoted the vigor of aged seeds in various rice varieties. Our results provide timely theoretical and technical insights for the trait improvement of seed aging tolerance in rice.

Key words: auxin-responsive gene , reactive oxygen species , rice , seed aging , seed vigor

Shan Sun, Wenjun Li, Yanfen Fang, Qianqian Huang, Zhibo Huang, Chengjing Wang, Jia Zhao, Yongqi He, Zhoufei Wang. 水稻生长素上调小RNA基因OsSAUR33促进种子耐老化[J]. Journal of Integrative Agriculture, 2025, 24(1): 61-71.

Shan Sun, Wenjun Li, Yanfen Fang, Qianqian Huang, Zhibo Huang, Chengjing Wang, Jia Zhao, Yongqi He, Zhoufei Wang. Small auxin-up RNA gene OsSAUR33 promotes seed aging tolerance in rice[J]. Journal of Integrative Agriculture, 2025, 24(1): 61-71.

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水稻生长素上调小RNA基因OsSAUR33促进种子耐老化

Small auxin-up RNA gene OsSAUR33 promotes seed aging tolerance in rice

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