棉花乙烯转录因子GhERF91参与黄萎病菌的防御反应

doi:10.1016/j.jia.2023.07.022

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (10): 3328-3342.DOI: 10.1016/j.jia.2023.07.022

棉花乙烯转录因子GhERF91参与黄萎病菌的防御反应

收稿日期:2023-04-10 接受日期:2023-07-03 出版日期:2024-10-20 发布日期:2024-09-10

Cotton ethylene response factor GhERF91 is involved in the defense against Verticillium dahliae

Nurimanguli Aini¹, Yuanlong Wu¹, Zhenyuan Pan¹, Yizan Ma², Qiushuang An¹, Guangling Shui¹, Panxia Shao¹, Dingyi Yang², Hairong Lin¹, Binghui Tang³, Xin Wei⁴, Chunyuan You^{2, 3}, Longfu Zhu^2#, Dawei Zhang^4#, Zhongxu Lin^2#, Xinhui Nie^1#

¹ Key Laboratory of Oasis Eco-agriculture, Xinjiang Production and Construction Group/Agricultural College, Shihezi University, Shihezi 832003, China
²National Key Laboratory of Crop Genetic Improvement/College of Plant Sciences & Technology, Huazhong Agricultural University, Wuhan 430070, China
³ Cotton Research Institute, Shihezi Academy of Agricultural Science, Shihezi 832011, China
⁴ Institute of Economic Crops, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China

Received:2023-04-10 Accepted:2023-07-03 Online:2024-10-20 Published:2024-09-10
About author:#Correspondence Longfu Zhu, Tel: +86-27-87283955, E-mail: lfzhu@mail.hzau.edu.cn; Dawei Zhang, Tel: +86-571-63370369, E-mail: zbzdw012@126.com; Zhongxu Lin, E-mail: linzhongxu@mail.hzau.edu.cn; Xinhui Nie, Tel: +86-993-2058970, E-mail: xjnxh2004130@126.com
Supported by:
This work was supported by the fund for National Key Research and Development Program of China (2023YFD2301203-05), the BTNYGG, China (NYHXGG, 2023AA102) and the Key Programs for Science and Technology Development of Shihezi City, Xinjiang Production and Construction Corps, China (2022NY01).

摘要/Abstract

摘要：

棉花黄萎病使棉花生产受到重大损失。为了揭示棉花响应黄萎病的防御机制，本研究利用棉花品系M138（抗黄萎病）和P2（感黄萎病）进行了转录组分析。结果显示，在M138和P2中分别有11076个和6640个差异表达基因响应黄萎病菌的侵染。利用加权基因共表达网络分析方法对4633个差异表达转录因子进行共表达分析发现，一个与黄萎病菌的抗性反应密切相关的“MEblue”模块，该模块包含654个转录因子。并在这些差异表达转录因子中，发现了参与黄萎病防御反应的关键基因乙烯转录因子GhERF91。进一步，通过病毒诱导的基因沉默实验和外源乙烯利处理分析发现，GhERF91受乙烯诱导表达，并正调控棉花对黄萎病菌的响应。本研究提供了棉花响应黄萎病侵染的转录组数据，同时鉴定了与黄萎病抗性相关的关键基因GhERF91，为选育抗黄萎病的棉花品种提供了的遗传资源。

Abstract:

Verticillium dahliae causes significant losses in cotton production. To reveal the mechanism of the defense response to V. dahliae in cotton, transcriptomic analyses were performed using cotton cultivars M138 (V. dahliae-resistant) and P2 (V. dahliae-susceptible). The results revealed 11,076 and 6,640 differentially expressed genes (DEGs) in response to V. dahliae, respectively. The weighted gene co-expression network analysis of 4,633 transcription factors (TFs) indicated a “MEblue” module containing 654 TFs that strongly correlate with resistance to V. dahliae. Among these TFs, the ethylene response factor Ghi_A05G10166 (GhERF91) was identified as a putative hub gene with a defense response against V. dahliae. A virus-induced gene silencing assay and exogenous application of ethephon showed that GhERF91 is activated by ethylene and positively regulates the response to V. dahliae exposure in cotton. This study provides fundamental transcriptome data and a putative causal gene (GhERF91) associated with resistance to V. dahliae, as well as genetic resources for breeding V. dahliae-resistant cotton.

Key words: cotton , Verticillium wilt , RNA-sequencing , GhERF91 , virus-induced gene silencing

Nurimanguli Aini, Yuanlong Wu, Zhenyuan Pan, Yizan Ma, Qiushuang An, Guangling Shui, Panxia Shao, Dingyi Yang, Hairong Lin, Binghui Tang, Xin Wei, Chunyuan You, Longfu Zhu, Dawei Zhang, Zhongxu Lin, Xinhui Nie. 棉花乙烯转录因子GhERF91参与黄萎病菌的防御反应[J]. Journal of Integrative Agriculture, 2024, 23(10): 3328-3342.

Nurimanguli Aini, Yuanlong Wu, Zhenyuan Pan, Yizan Ma, Qiushuang An, Guangling Shui, Panxia Shao, Dingyi Yang, Hairong Lin, Binghui Tang, Xin Wei, Chunyuan You, Longfu Zhu, Dawei Zhang, Zhongxu Lin, Xinhui Nie. Cotton ethylene response factor GhERF91 is involved in the defense against Verticillium dahliae[J]. Journal of Integrative Agriculture, 2024, 23(10): 3328-3342.

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棉花乙烯转录因子GhERF91参与黄萎病菌的防御反应

Cotton ethylene response factor GhERF91 is involved in the defense against Verticillium dahliae

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