从中国某鸭场中鉴定出blaIMI介导的碳青霉烯类耐药肠杆菌属细菌

doi:10.1016/j.jia.2023.06.013

摘要/Abstract

摘要：

近年来，碳青霉烯耐药肠杆菌成为了一个威胁临床抗生素治疗的问题。肠杆菌属细菌作为腐生多宿主细菌，在环境、畜禽和人之间广泛存在，调查畜禽养殖生产过程中的碳青霉烯耐药肠杆菌属细菌，对预防和遏制肠杆菌属细菌碳青霉烯类耐药具有重要意义。

本研究从中国某养鸭场的鸭肠道与环境样本中，分离出携带bla_IMI的碳青霉烯类和黏菌素耐药肠杆菌属细菌。药敏试验显示，四株bla_IMI阳性肠杆菌属细菌分离株对碳青霉烯和黏菌素具有耐药性。PCR和Sanger测序证明了在不同ST型的菌株中检测到了三种bla_IMI亚型。全基因组测序证明bla_IMI基因存在于这些菌株的染色体或质粒中。接合转移实验证明了携带bla_IMI的质粒具有水平传播的能力。我们运用比较分析手段对其分子进化特征进行了研究，发现两株bla_IMI-16阳性阿氏肠杆菌之间具有高度相似的基因环境，但有着较远的亲缘关系，这提示了bla_IMI-16水平传播的可能。此外，携带bla_IMI-16的质粒是IncFⅠⅠ(Yp)质粒，该型质粒能够使宿主菌具备生长的竞争性优势。在该质粒上，bla_IMI-16的上下游鉴定出大量的可移动遗传元件（MGEs）序列，以IS1081、Tn903和ISE居多。本研究中的bla_IMI阳性肠杆菌属细菌中还携带有毒力基因，有助于增强菌株致病性。

综上所述，我们发现了在中国某鸭场内的肠杆菌属细菌中，bla_IMI由IncFⅠⅠ(Yp)质粒携带并促进其传播，从而导致了细菌碳青霉烯耐药性的传播。可移动遗传元件在水平传播中发挥着重要作用。对于bla_IMI基因的研究较少，此前，从未在中国大陆的养殖源细菌中发现，也未见有bla_IMI_-₁₆基因在中国大陆被鉴定的报道。我们的研究为不同亚型bla_IMI基因在中国的传播提供了证据，并为研究bla_IMI阳性耐药肠杆菌属细菌提供了参考数据。本研究强调：我们仍需要定期监测动物饲养过程中的bla_IMI阳性肠杆菌属细菌，以预防及遏制日益严重的碳青霉烯类药物耐药性传播的威胁。

Abstract:

Carbapenem- and colistin-resistant Enterobacter has been a clinical and therapy problem in recent years. Here, we report the carbapenem- and colistin-resistant Enterobacter harboring blaIMI isolated from intestinal samples and the environment of a duck farm in China. Four blaIMI-positive Enterobacter isolates were resistant to carbapenem and colistin. Three blaIMI subtypes were detected in different molecular categories of Enterobacter. The detection of the various IMI producers highlights the diversity of carbapenemases in a duck farm. Whole-genome sequencing demonstrated the blaIMI genes were present in chromosomes or plasmids in these strains. The conjugation experiment demonstrated the ability of blaIMI-carrying plasmid to transmit horizontally. The molecular evolution characteristics were examined through comparative genetic analysis. The study demonstrated the presence of chromosomal and plasmid blaIMI and the blaIMI-carrying plasmid exhibits a horizontal transmission between Enterobacter and Escherichia coli C600. The similar genetic content was discovered between two blaIMI-16-positive Enterobacter asburiae. In addition, a blaIMI-16-carrying plasmid is an IncFII(Yp) plasmid, and a substantial amount of mobile genetic elements were identified around blaIMI-16. The IS-like elements and IncFII(Yp) plasmid are significant in the propagation of blaIMI. Our study provides evidence for the transmission of diverse blaIMI genes in China and supplies additional reference data for blaIMI-positive antimicrobial-resistant Enterobacter. Routine surveys of blaIMI-positive Enterobacter from animal-raising environments must be given more focus

Key words: Enterobacter , antimicrobial resistance , carbapenemases , colistin

HUANG Hong-hao, LU Yi-xing, WU Su-juan, MA Zhen-bao, ZENG Dong-ping, ZENG Zhen-ling. 从中国某鸭场中鉴定出bla_IMI介导的碳青霉烯类耐药肠杆菌属细菌[J]. Journal of Integrative Agriculture, 2023, 22(8): 2500-2508.

HUANG Hong-hao, LU Yi-xing, WU Su-juan, MA Zhen-bao, ZENG Dong-ping, ZENG Zhen-ling. Identification of bla_IMI-mediated carbapenem-resistant Enterobacter from a duck farm in China[J]. Journal of Integrative Agriculture, 2023, 22(8): 2500-2508.

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