Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (2): 621-638.DOI: 10.1016/j.jia.2023.06.007

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昆虫病原真菌爪哇棒束孢菌和低剂量的呋虫胺对水稻害虫白背飞虱的高效协同控制

  

  • 收稿日期:2023-02-20 接受日期:2023-04-21 出版日期:2024-02-20 发布日期:2024-01-30

Synergistic effects of the entomopathogenic fungus Isaria javanica and low doses of dinotefuran on the efficient control of the rice pest Sogatella furcifera

Tingting Zhou1*, Qian Zhao1*, Chengzhou Li1, Lu Ye1, Yanfang Li2#, Nemat O. Keyhani3, Zhen Huang1#   

  1. 1 Department of Entomology, College of Plant Protection, South China Agricultural University, Guangzhou 510642, China

    2 Guangdong Provincial Key Laboratory of High Technology for Plant Protection, Plant Protection Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510642, China

    3 Department of Microbiology and Cell Science, Institute of Food and Agricultural Sciences, University of Florida, FL 32611, USA

  • Received:2023-02-20 Accepted:2023-04-21 Online:2024-02-20 Published:2024-01-30
  • About author:Tingting Zhou, E-mail: 1600468609@qq.com; Qian Zhao, E-mail: 861962790@qq.com; #Correspondence Yanfang Li, Tel: +86-20-87511154, E-mail: 328159805@qq.com; Zhen Huang, Tel: +86-20-85286089, E-mail: hzscau@scau.edu.cn * These authors contributed equally to this study.
  • Supported by:

    This study was funded by grants from the Science and Technology Planning Project of Guangzhou, China (202002020029), the Science and Technology Planning Project of Guangdong Province, China (2019B020217003), the National Key R&D Program of China (2018YFD02003), and the National Key Technology Support Program of China (201303019-02).

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摘要：

白背飞虱是水稻上的一种刺吸式口器类害虫，现已对常用的许多化学农药产生了中等到高强度的抗药性，严重发生时可导致水稻大面积减产。我们在室内和田间条件下研究了爪哇棒束孢菌单独、或与化学农药呋虫胺混合对白背飞虱的影响，研究结果表明：爪哇棒束孢菌可高效地侵染、杀死不同虫态的白背飞虱，可降低成虫的成活率、产卵量、影响卵巢的发育。室内生物测定结果表明爪哇棒束孢菌与低剂量的呋虫胺（4-16ppm）的混合物对白背飞虱的死亡率高于单独使用爪哇棒束孢菌或呋虫胺对白背飞虱的死亡率，并可显著地影响害虫的过氧化物歧化酶、过氧化氢酶、过氧化物酶、酚氧化酶等酶的活性。在田间爪哇棒束孢菌可有效地降低白背飞虱的种群数量（田间虫量只有对照区的22-64%），低剂量的爪哇棒束孢菌（1×104 孢子 mL-1）和呋虫胺（4.8-19.2%的田间推荐用量）在田间对白背飞虱的防治效果超过90%、表现为协同增效作用，在使用后的第3-14天内可将田间白背飞虱的种群数量持续控制在50头/100丛以下。我们的研究结果表明：昆虫病原真菌爪哇棒束孢菌可作为水稻害虫综合防治（IPM）的重要组成部分，用于控制水稻害虫。

Abstract: The rice planthopper, Sogatella furcifera, is a piercing-sucking insect pest of rice, Oryza sativa.  It is responsible for significant crop yield losses, and has developed moderate to high resistance to several commonly used chemical insecticides.  We investigated the effects of the insect fungal pathogen Isaria javanica, alone and in combination with the chemical insecticide dinotefuran, on S. furcifera under both laboratory and field conditions.  Our results show that I. javanica displays high infection efficiency and mortality for different stages of S. furcifera, reducing adult survival, female oviposition and ovary development.  Laboratory bioassays showed that the combined use of I. javanica with a low dose (4–16 mg L–1) of dinotefuran resulted in higher mortality in S. furcifera than the use of I. javanica or dinotefuran alone.  The combined treatment also had more significant effects on several host enzymes, including superoxide dismutase, catalase, peroxidase, and prophenol oxidase activities.  In field trials, I. javanica effectively suppressed populations of rice planthoppers to low levels (22–64% of the level in untreated plots).  Additional field experiments showed synergistic effects, i.e., enhanced efficiency, for the control of S. furcifera populations using the combination of a low dose of I. javanica (1×104 conidia mL–1) and a low dose of dinotefuran (~4.8–19.2% of normal field use levels), with control effects of >90% and a population level under 50 insects per 100 hills at 3–14 days post-treatment.  Our findings indicate that the entomogenous fungus I. javanica offers an attractive biological control addition as part of the integrated pest management (IPM) practices for the control of rice plant pests.

Key words: Isaria javanica ,  Sogatella furcifera ,  entomopathogenic fungus ,  white planthopper ,  rice ,  synergistic effect on pest control