创造小麦大规模EMS突变群体用于功能基因组学和育种应用

doi:10.1016/j.jia.2023.05.039

Journal of Integrative Agriculture ›› 2024, Vol. 23 ›› Issue (2): 484-493.DOI: 10.1016/j.jia.2023.05.039

创造小麦大规模EMS突变群体用于功能基因组学和育种应用

收稿日期:2023-01-31 接受日期:2023-05-15 出版日期:2024-02-20 发布日期:2024-01-29

Creating large EMS populations for functional genomics and breeding in wheat

Wenqiang Wang^{1, 2*}, Xizhen Guan^1*, Yong Gan³, Guojun Liu³, Chunhao Zou¹, Weikang Wang¹, Jifa Zhang¹, Huifei Zhang¹, Qunqun Hao², Fei Ni³, Jiajie Wu³, Lynn Epstein⁴, Daolin Fu^{1, 3#}

¹Spring Valley Agriscience Co., Ltd., Jinan 250300, China
²College of Life Sciences, Zaozhuang University, Zaozhuang 277160, China
³National Key Laboratory of Wheat Improvement, College of Agronomy, Shandong Agricultural University, Tai’an 271018, China
⁴Department of Plant Pathology, University of California, Davis, CA 95616, USA

Received:2023-01-31 Accepted:2023-05-15 Online:2024-02-20 Published:2024-01-29
About author:Wenqiang Wang, E-mail: wangwenqiang881202@163.com; Xizhen Guan, E-mail: xzhguan@svjn.com.cn; #Correspondence Daolin Fu, Tel: +86-531-88766999, E-mail: dlfu@svjn.com.cn, dlfu@sdau.edu.cn
Supported by:
This work was supported by the National Key Research and Development Program of China (2022YFF1002300), the Quancheng ‘5150’ Talent Program, China (07962021047) and the Agriculture Applied Technology Initiative of Jinan Government, China (CX202113).

摘要/Abstract

摘要：

小麦种质资源是基础研究、应用研究和小麦遗传育种的基础资源，可以通过收集野生资源、积累育种品系、引入诱变材料等多种途径获得。甲基磺酸乙酯（EMS）是一种烷化剂，在植物中可以有效的诱导产生遗传变异。本研究中，我们以中国的小麦品种“鲁研128”、“济麦38”、“济麦44”和“山农30”亲本，创制了包含百万级的EMS突变群体。在M₁代中，突变群体的表型变异丰富，鉴定到>3000个叶绿素缺失突变体、2519个密穗突变体和1692个雄性不育突变体。此外，我们也鉴定到一些稀有突变，例如30个双穗突变。遗传学分析发现M₁代中部分叶绿素缺失和密穗突变体在M₂或M₃都能稳定遗传。为了更好的保留该突变群体达到更高的世代，我们设计了单粒传的保存方法。目前，我们正在用M₂突变群体筛选其它重要的农艺性状，如抗精喹禾灵除草剂的突变体。本突变群体可用于科研合作，旨在为小麦的基础研究和遗传育种提供一个有价值的工具。

Abstract:

Wheat germplasm is a fundamental resource for basic research, applied studies, and wheat breeding, which can be enriched normally by several paths, such as collecting natural lines, accumulating breeding lines, and introducing mutagenesis materials. Ethyl methane sulfonate (EMS) is an alkylating agent that can effectively introduce genetic variations in a wide variety of plant species. In this study, we created a million-scale EMS population (MEP) that started with the Chinese wheat cultivars ‘Luyan 128’, ‘Jimai 38’, ‘Jimai 44’, and ‘Shannong 30’. In the M₁ generation, the MEP had numerous phenotypical variations, such as >3,000 chlorophyll-deficient mutants, 2,519 compact spikes, and 1,692 male sterile spikes. There were also rare mutations, including 30 independent tillers each with double heads. Some M₁ variations of chlorophyll-deficiency and compact spikes were inheritable, appearing in the M₂ or M₃ generations. To advance the entire MEP to higher generations, we adopted a single-seed descendent (SSD) approach. All other seed composites of M₂ were used to screen other agronomically important traits, such as the tolerance to herbicide quizalofop-P-methyl. The MEP is available for collaborative projects, and provides a valuable toolbox for wheat genetics and breeding for sustainable agriculture.

Key words: wheat , germplasm , ethyl methane sulfonate , genetics and breeding

. 创造小麦大规模EMS突变群体用于功能基因组学和育种应用[J]. Journal of Integrative Agriculture, 2024, 23(2): 484-493.

Wenqiang Wang, Xizhen Guan, Yong Gan, Guojun Liu, Chunhao Zou, Weikang Wang, Jifa Zhang, Huifei Zhang, Qunqun Hao, Fei Ni, Jiajie Wu, Lynn Epstein, Daolin Fu.

Creating large EMS populations for functional genomics and breeding in wheat [J]. Journal of Integrative Agriculture, 2024, 23(2): 484-493.

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创造小麦大规模EMS突变群体用于功能基因组学和育种应用

Creating large EMS populations for functional genomics and breeding in wheat

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