GbLMI1过表达促进棉花地上部营养生长

doi:10.1016/j.jia.2023.05.037

摘要/Abstract

摘要： 棉花叶片是光合作用和有机物质合成的主要场所。叶形对光合效率和冠层形成具有重要影响，进而影响棉花产量。研究表明LMI1是调控叶形的关键基因。本研究利用35S启动子构建LMI1基因过表达载体，转化至棉花并获得了LMI1过表达植株。对过表达T₁和T₂代植株田间性状统计分析，发现过表达植株叶片显著增大、茎变粗和干重显著增加。叶脉和叶柄的徒手切片观察发现过表达植株细胞数量显著增加。此外，转录组分析发现赤霉素合成通路和NAC基因家族相关的基因差异表达，推测LMI1参与了细胞壁形成和细胞增殖，进而促进了茎变粗。GO富集分析在钙离子结合条目，KEGG富集分析在脂肪酸降解、磷脂酰肌醇信号转导系统和cAMP信号途径通路。结果表明，LMI1过表达植株响应赤霉素信号，通过第二信使信号（cAMP，CA²⁺），进而增强功能，促进植物营养生长。本研究为探究LMI1促进棉花营养生长，进而提高产量提供理论基础。

Abstract:

Leaves are the main organ for photosynthesis and organic synthesis in cotton. Leaf shape has important effects on photosynthetic efficiency and canopy formation, thereby affecting cotton yield. Previous studies have shown that LMI1 (LATE MERISTEM IDENTITY1) is the main gene regulating leaf shape. In this study, the LMI1 gene was inserted into the 35S promoter expression vector, and cotton plants overexpressing LMI1 (OE) were obtained through genetic transformation. Statistical analysis of the biological traits of the T₁ and T₂ populations showed that compared to the wild type (WT), OE plants had significantly larger leaves, thicker stems and significantly greater dry weight. Furthermore, plant sections of the main vein and petiole showed that the numbers of cells in those tissues of OE plants were significantly greater. In addition, RNA-seq analysis revealed the differential expression of genes related to gibberellin synthesis and NAC gene family (genes containing the NAC domain) between the OE and WT plants, suggesting that LMI1 is involved in secondary wall formation and cell proliferation, which promotes stem thickening. Moreover, Gene Ontology (GO) analysis revealed enrichment in the terms of calcium ion binding, and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed enrichment in the terms of fatty acid degradation, phosphatidylinositol signal transduction system, and cAMP (cyclic adenosine monophosphate) signal pathway. These results suggested that LMI1 OE plants are responsive to gibberellin hormone signals, and have altered messenger signals (cAMP, Ca²⁺) which amplify this function, to promote stronger aboveground vegetative growth. This study found the LMI1 greatly increased the vegetative growth in cotton, which is the basic requirement for higher yield.

Key words: LMI1 , over-expressing , cotton , vegetative growth

Zhili Chong, Yunxiao Wei, Kaili Li, Muhammad Aneeq Ur Rahman, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Liangrong He, Rui Zhang.

GbLMI1过表达促进棉花地上部营养生长 [J]. Journal of Integrative Agriculture, 2024, 23(10): 3457-3467.

Zhili Chong, Yunxiao Wei, Kaili Li, Muhammad Aneeq Ur Rahman, Chengzhen Liang, Zhigang Meng, Yuan Wang, Sandui Guo, Liangrong He, Rui Zhang. GbLMI1 over-expression improves cotton aboveground vegetative growth[J]. Journal of Integrative Agriculture, 2024, 23(10): 3457-3467.

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