香蕉穿孔线虫的分支酸变位酶在线虫致病性中起重要作用

doi:10.1016/j.jia.2023.04.040

摘要/Abstract

摘要：

植物寄生线虫在侵染植物的过程中通过口针或者体壁向植物细胞和组织中释放对成功侵染致病发挥重要作用的一系列蛋白。本研究利用香蕉穿孔线虫（Radopholus similis）转录组数据鉴定克隆了香蕉穿孔线虫分支酸变位酶（RsCM）基因。该基因在香蕉穿孔线虫的食道腺特异表达，且在雌虫和幼虫中高表达，为单拷贝基因。在烟草叶片中的瞬时表达，结果表明RsCM定位在烟草叶片细胞的细胞质和细胞核，能够抑制flg22诱导的包括胼胝质沉积和防卫基因表达的PTI反应，并能够抑制BAX诱导的细胞坏死，但不能抑制Gpa2/RBP-1 诱导的细胞坏死。RsCM的RNAi转基因番茄对香蕉穿孔线虫的侵染致病和繁殖表现明显抑制作用；而过表达转基因番茄对香蕉穿孔线虫侵染的抵抗力明显降低，并且病根中与水杨酸途径相关的2个基因PR1和PR5的表达量显著下调，表明RsCM可能参与了水杨酸途径的抑制。本研究结果表明，RsCM抑制了寄主的免疫系统，为防治香蕉穿孔线虫提供了新的靶标，同时也为研究迁移性寄生植物线虫分支酸变位酶基因的功能和作用机制提供了新的数据。

Abstract:

In the process of infecting plants, plant parasitic nematodes release a series of proteins that play an essential role in the successful infection and pathogenesis of plant cells and tissues through stylets or body walls. In this study, based on transcriptome data, a chorismate mutase gene of Radopholus similis (RsCM) was identified and cloned, which is a single copy gene specifically expressed in the oesophageal gland and highly expressed in juveniles and females. Transient expression of RsCM in tobacco leaves showed that it was localised in the cytoplasm and nucleus of tobacco leaf cells, which inhibited the pattern-triggered immunity (PTI) induced by flg22, including callose deposition and defence gene expression, and cell death induced by immune elicitors BAX, but could not inhibit cell death induced by immune elicitors Gpa2/RBP-1. The RNA interference (RNAi) transgenic tomato of RsCM obviously inhibited the infection, pathogenicity, and reproduction of R. similis. However, the resistance of the overexpression transgenic tomato of RsCM to R. similis infection was significantly reduced, and the expression levels of two salicylic acid (SA) pathway genes (PR1 and PR5) in roots infected by the nematode were significantly down-regulated, which indicated that RsCM might be involved in the inhibition of SA pathway. The results of this study demonstrate that RsCM suppresses the host immune system and might be a new target for the control of R. similis, which also provides new data for the function and mechanism of CM genes of migratory parasitic plant nematodes.

Key words: Radopholus similis , chorismate mutase , plant defense , transgenic tomato

. 香蕉穿孔线虫的分支酸变位酶在线虫致病性中起重要作用[J]. Journal of Integrative Agriculture, 2024, 23(3): 923-937.

Sihua Yang, Junyi Li, Shuai Yang, Shiqiao Tang, Huizhong Wang, Chunling Xu, Hui Xie.

A chorismate mutase from Radopholus similis plays an essential role in pathogenicity [J]. Journal of Integrative Agriculture, 2024, 23(3): 923-937.

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