cAMP和钙离子信号循环效应对枣生长发育的影响

doi:10.1016/j.jia.2023.04.039

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (7): 2094-2110.DOI: 10.1016/j.jia.2023.04.039

cAMP和钙离子信号循环效应对枣生长发育的影响

收稿日期:2022-12-22 接受日期:2023-03-24 出版日期:2023-07-20 发布日期:2023-03-24

A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development

WANG Li-xin^1,²^*, WANG Lin-xia¹^,³^*, ZHANG Meng-ling^1*, QU Ying-yue^1*, YUAN Ye¹, Ehsan SADEGHNEZHAD⁴, GAO Meng-jiao¹, ZHAO Ruo-yu¹, QI Chao-feng¹, GUO Xiao-xue¹, ZHU Wen-hui¹, LI Rui-mei¹, DAI Li^1,², LIUMeng-jun^1,², LIU Zhi-guo^1,^2#

¹ College of Horticulture, Hebei Agricultural University, Baoding 071001, P.R.China
² Research Center of Chinese Jujube, Hebei Agricultural University, Baoding 071001, P.R.China
³ Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education/College of Life Sciences,
Northwest University, Xi’an 710069, P.R.China
⁴ Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, Teheran 14115, Iran

Received:2022-12-22 Accepted:2023-03-24 Online:2023-07-20 Published:2023-03-24
About author:#Correspondence LIU Zhi-guo, Tel/Fax: +86-312-7528962, E-mail: jujubeliu@163.com * These authors contributed equally to this study.
Supported by:
This research was funded by the Provincial Supporting Program of Hebei for the Returned Oversea Scholars, China (C20210114), the Science and Technology Project of Hebei Education Department (QN2022017), the Fundamental Scientific Research Fund of Universities in Hebei Province (KY2021059), the China Agriculture Research System (CARS-30-2-07), the National Key Research and Development Project of China (2019YFD1001605), the Natural Science Foundation of Hebei Province (C2020204082), the Funds for Hebei Jujube Industry Technology Research Institute after Operation Performance (205676155H), and the Young Talent Project of Hebei Agricultural University Foundation (YJ201853).

摘要/Abstract

摘要：

3',5'-环磷酸腺苷（cAMP）是一种重要的代谢产物，特别在枣中高效积累。然而，cAMP在枣细胞中的功能尚未得到系统研究。因此，我们通过枣细胞悬浮系的建立、原生质体分离及荧光强度分析研究了cAMP与钙离子信号的关系。首先，外源cAMP处理可以促进枣的生长和内源cAMP的积累。通过对过表达腺苷酸环化酶（ZjAC）的转基因拟南芥转录组分析鉴定出60个与钙离子信号相关的差异表达基因（DEG），发现这些基因参与钙离子信号转导及细胞间/内的反应。另外，外源cAMP和钙离子促进剂A23187等药物处理可以诱导枣细胞中ZjAC的表达、cAMP的积累及钙离子向细胞质中的流入，而钙离子螯合剂EGTA或腺苷酸环化酶抑制剂bithionol处理抑制了这种增加。此外，外源cAMP处理可以激活钙离子通道及相关下游基因，如ZjCNGC2和ZjMAPK2、ZjMAPKK2、ZjMAPKK4。总之，该研究结果表明cAMP的合成依赖于钙离子信号内流，钙离子信号和cAMP之间的级联放大效应参与细胞内信号转导从而促进枣的生长发育。

Abstract: 3´,5´-Cyclic adenosine monophosphate (cAMP) is an important metabolite that is specifically enriched in jujube. However, the effect of cAMP on jujube cellular responses has not been comprehensively studied. Here, we established jujube cell suspension cultures and investigated the calcium influx in response to cAMP treatment through protoplast isolation and fluorescence intensity. Firstly, cAMP treatment could promote jujube growth and increase the content of endogenous cAMP. Using transcriptome analysis with transgenic Arabidopsis plants overexpressing adenylate cyclase (ZjAC) as a positive control, we identified 60 calcium-related differential expressed genes (DEGs) that contributed to the calcium signaling and inter- or intra-cellular responses. Pharmacological treatments such as cAMP and the calcium ionophore A23187 could induce ZjAC expression, the accumulation of cAMP and calcium influx in jujube cells, while ethylene glycol tetraacetic acid (EGTA) or bithionol treatment inhibited these changes. Moreover, the calcium channels and transporters in calcium influx, such as the ZjCNGC2 channel and the mitogen activated protein (MAP) kinase pathway, could be activated by cAMP treatment. In summary, our findings demonstrated that cAMP biosynthesis is dependent on calcium influx and the amplifying effect between calcium and cAMP may be involved in intracellular signal induction, which might contribute to the growth and development of jujube.

Key words: cAMP , calcium , growth , suspension cells , protoplast , signaling , jujube

WANG Li-xin, WANG Lin-xia, ZHANG Meng-ling, QU Ying-yue, YUAN Ye, Ehsan SADEGHNEZHAD, GAO Meng-jiao, ZHAO Ruo-yu, QI Chao-feng, GUO Xiao-xue, ZHU Wen-hui, LI Rui-mei, DAI Li, LIU Meng-jun, LIU Zhi-guo. cAMP和钙离子信号循环效应对枣生长发育的影响[J]. Journal of Integrative Agriculture, 2023, 22(7): 2094-2110.

WANG Li-xin, WANG Lin-xia, ZHANG Meng-ling, QU Ying-yue, YUAN Ye, Ehsan SADEGHNEZHAD, GAO Meng-jiao, ZHAO Ruo-yu, QI Chao-feng, GUO Xiao-xue, ZHU Wen-hui, LI Rui-mei, DAI Li, LIU Meng-jun, LIU Zhi-guo. A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development[J]. Journal of Integrative Agriculture, 2023, 22(7): 2094-2110.

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cAMP和钙离子信号循环效应对枣生长发育的影响

A cyclic effect of cAMP and calcium signaling contributes to jujube growth and development

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