Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (7): 1951-1966.DOI: 10.1016/j.jia.2023.02.031

• •    下一篇

叶绿体遗传转化技术的优化方法和工具箱

  

  • 收稿日期:2022-11-18 接受日期:2023-01-12 出版日期:2023-07-20 发布日期:2023-01-12

Advancing approach and toolbox in optimization of chloroplast genetic transformation technology

LIU Yu-xin1, 2, LI Fan1, 2, GAO Liang1, 3, TU Zhang-li1, 2, ZHOU Fei1, 2#, LIN Yong-jun1, 2   

  1. 1 National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, P.R.China

    2 Hubei Hongshan Laboratory, Wuhan 430070, P.R.China.

    3 Wuhan Towin Biotechnology Co., Ltd., Wuhan 430070, P.R.China

  • Received:2022-11-18 Accepted:2023-01-12 Online:2023-07-20 Published:2023-01-12
  • About author:#Correspondence ZHOU Fei, Tel: +86-27-87280550, E-mail:zhoufei@mail.hzau.edu.cn
  • Supported by:
    This work was funded by the Foundation of Hubei Hongshan Laboratory, China (2022hszd014) and the National Natural Science Foundation of China (31771752).

摘要:

叶绿体作为一种离散、高度结构化、半自主的细胞器。叶绿体的小基因组能成为合成生物学极有前途的平台。叶绿体基因工程作为一种特殊的合成生物学手段,对重建植物体内各种复杂的代谢途径具有巨大的潜力,例如提高作物的光合能力,植物的抗逆性或者合成新的药物和疫苗。然而,许多植物的叶绿体遗传转化效率低或无法进行。因此,新的转化技术和工具正在不断被开发。为了进一步拓展和促进叶绿体基因工程的应用,本文综述了近年来在叶绿体遗传转化方面的新技术,并对选择合适的合成生物元件用以构建高效的叶绿体转化载体进行了探讨。

Abstract:

Chloroplast is a discrete, highly structured, and semi-autonomous cellular organelle. The small genome of chloroplast makes it an up-and-coming platform for synthetic biology. As a special means of synthetic biology, chloroplast genetic engineering shows excellent potential in reconstructing various sophisticated metabolic pathways within the plants for specific purposes, such as improving crop photosynthetic capacity, enhancing plant stress resistance, and synthesizing new drugs and vaccines. However, many plant species exhibit limited efficiency or inability in chloroplast genetic transformation. Hence, new transformation technologies and tools are being constantly developed. In order to further expand and facilitate the application of chloroplast genetic engineering, this review summarizes the new technologies in chloroplast genetic transformation in recent years and discusses the choice of appropriate synthetic biological elements  for the construction of efficient chloroplast transformation vectors.

Key words: chloroplast ,  genetic engineering ,  new technology ,  plasmid optimization ,  nanotechnology