Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (8): 2470-2482.DOI: 10.1016/j.jia.2023.02.028

• • 上一篇    下一篇

NlMIP和Gαi/q偶联受体NlA10的结合促进雌性褐飞虱腹部振动的产生

  

  • 收稿日期:2022-09-24 接受日期:2022-12-20 出版日期:2023-08-20 发布日期:2022-12-20

The combination of NlMIP and Gαi/q coupled-receptor NlA10 promotes abdominal vibration production in female Nilaparvata lugens (Stål)

SU Qin1, 2, LÜ Jun1, LI Wan-xue1, CHEN Wei-wen1, LUO Min-shi1, ZHANG Chuan-chuan1, ZHANG Wen-qing1#   

  1. 1 State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, P.R.China

    2 Department of Forensic Medicine, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou 510080, P.R.China

  • Received:2022-09-24 Accepted:2022-12-20 Online:2023-08-20 Published:2022-12-20
  • About author:SU Qin, E-mail: 470457020@qq.com; #Correspondence ZHANG Wen-qing, Tel: +86-20-39332963, Fax: +86-20-39943515, E-mail: lsszwq@mail.sysu.edu.cn
  • Supported by:

    The study was funded by the National Natural Science Foundation of China (31730073).

PDF

摘要：

对于某些性成熟昆虫（包括褐飞虱）而言，腹部振动（abdominal vibration，AV）是交配过程的启动信号，对成功交配至关重要。目前，关于调控AV的遗传和分子机制的研究很少。我们以往与AV相关的转录组学研究表明，肌抑制肽（myoinhibitory peptide，NlMIP）是调控雌性褐飞虱AV的潜在基因，但其对AV的调控机制尚未报道。本文证实了NlMIP参与调控雌性褐飞虱的AV和交配行为。当NlMIP的敲低效率为59.00%时，雌性褐飞虱在1小时内产生AV的频率和交配成功率分别下降了38.89%和61.11%。此外，6条NlMIP成熟肽也能够调控雌性褐飞虱AV的产生及其交配行为，其中NlMIP2的作用最强。基于系统发育树分析和NlMIP成熟肽能有效激活A家族神经肽G蛋白偶联受体10（A-family neuropeptide GPCR 10，NlA10）的结果表明NlA10是NlMIP的潜在受体。NlA10被敲低后，雌性褐飞虱1小时内产生AV的频率和交配成功率分别下降了28.89%和43.33%。当NlA10被NlMIP2激活时，NlA10偶联到Gαi/q信号通路，从而抑制下游AC/cAMP/PKA，并激活PLC/Ca2+/PKC信号通路，进而级联激活MEK1/2介导ERK1/2的磷酸化，最终调控雌性褐飞虱的AV。这些结果为通过干扰雌性褐飞虱的AV进行害虫防治提供了依据。

Abstract:

For various sexually mature insects, including the brown planthopper (BPH, Nilaparvata lugens), the abdominal vibration (AV) signal is the initiation of the mating process, and it is critical to the success of mating.  Currently, there are few studies on the genetic and molecular mechanisms of AV regulation.  Our previous AV-related transcriptomic study in female BPH identified myoinhibitory peptide (NlMIP) as a gene that potentially affects AV status in females, but how NlMIP affects AV status remains unknown.  In this study, we confirmed that NlMIP regulates AV production and mating behavior in female BPH.  When the RNAi knockdown efficiency of NlMIP was 59.00%, the probability of females producing AV and the mating rate in 1 h decreased by 38.89 and 61.11%, respectively.  In addition, six mature peptides of NlMIP were synthesized and they were able to regulate AV production and mating behavior in females, with NlMIP2 having the strongest effect.  The A-family neuropeptide GPCR 10 (NlA10) was found to be a potential receptor for NlMIP based on a phylogenetic tree analysis and the fact that NlMIP mature peptides effectively activated NlA10.  After NlA10 was knocked down, the probability of females producing AV and the mating rate in 1 h had reductions of 28.89 and 43.33%, respectively.  When activated by NlMIP2, NlA10 coupled the Gαi/q signalling pathways, thereby inhibiting the downstream AC/cAMP/PKA, activating the PLC/Ca2+/PKC signalling pathways and then activating MEK1/2 in a cascade to mediate the phosphorylation of ERK1/2, and finally regulating the AV of females.  These results provide a basis for the prevention and control of the brown planthopper pest by disrupting female AV.

Key words: abdominal vibration ,  mating disruption ,  myoinhibitory peptide (MIP) ,  NlA10 ,  GPCR ,  brown planthopper