玉米糖转运蛋白家族基因的鉴定和表达分析以及ZmSTP2和ZmSTP20在抗病中的作用

doi:10.1016/j.jia.2022.12.014

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (11): 3458-3473.DOI: 10.1016/j.jia.2022.12.014

玉米糖转运蛋白家族基因的鉴定和表达分析以及ZmSTP2和ZmSTP20在抗病中的作用

收稿日期:2022-09-16 接受日期:2022-11-09 出版日期:2023-11-20 发布日期:2023-11-08

Identification and expression analysis of sugar transporter family genes reveal the role of ZmSTP2 and ZmSTP20 in maize disease resistance

MA Yu-xin^{1, 2*}, ZHOU Zhi-jun^4*, CAO Hong-zhe^{1, 2}, ZHOU Fan^{1, 2}, SI He-long², ZANG Jin-ping², XING Ji-hong^{1, 2, 3#}, ZHANG Kang^{1, 2, 3#}, DONG Jin-gao^{1, 2#}

¹State Key Laboratory of North China Crop Improvement and Regulation, Hebei Agricultural University, Baoding 071000, P.R.China
²Hebei Key Laboratory of Plant Physiology and Molecular Pathology, Hebei Agricultural University, Baoding 071000, P.R.China
³ Hebei Bioinformatic Utilization and Technological Innovation Center for Agricultural Microbes, Hebei Agricultural University, Baoding 071000, P.R.China
⁴Experimental Training Center of Hebei Agricultural University, Hebei Agricultural University, Baoding 071000, P.R.China

Received:2022-09-16 Accepted:2022-11-09 Online:2023-11-20 Published:2023-11-08
About author:#Correspondence XING Ji-hong, E-mail: xingjihong2000@126.com; ZHANG Kang, E-mail: zk7588@163.com; DONG Jin-gao, E-mail: dongjingao@126.com * These authors contributed equally to this study.
Supported by:
This work was supported by the National Natural Science Foundation of China (31901864), the State Key Laboratory of North China Crop Improvement and Regulation (NCCIR2020ZZ-9), the Research Project of Science and Technology in Universities of Hebei Province (BJK2022006), earmarked fund for China Agriculture Research System (CARS-02), the Key Research and Development Projects of Hebei (19226503D), and the Central Government Guides Local Science and Technology Development Projects (216Z6501G, 216Z6502G).

摘要/Abstract

摘要：

糖是植物生长发育中不可或缺的生长能源，在通过疏水屏障时需要糖转运蛋白（STP）的帮助。当玉米受到病原体侵染时，糖的含量会产生波动，但是糖转运蛋白如何在玉米抗病过程中发挥作用还没有清晰地研究。为了鉴定玉米(Zea mays)糖转运蛋白家族成员并分析其在不同组织和生理条件下的表达规律，本研究利用生物信息学方法对玉米基因组中糖转运蛋白编码基因进行了系统性鉴定和分析，利用同源性分析鉴定玉米糖转运蛋白编码基因，利用保守结构域分析对糖转运蛋白进行结构域鉴定，利用转录组数据对糖转运蛋白编码基因进行表达规律分析，利用激素处理试验，验证了该家族成员在激素处理下的表达规律，并利用病斑侵染突变体，验证了该家族成员ZmSTP2和ZmSTP20具有抗病性。结果表明，玉米糖转运蛋白家族包含24个成员，均预测分布在细胞膜上，具有高度保守的跨膜转运结构域，玉米糖转运蛋白编码基因在不同组织中和胁迫下表达水平有明显差异，其中ZmSTP2和ZmSTP20在禾谷镰孢（Fusarium graminearum）侵染后表达水平持续上升，通过对zmstp2和zmstp20突变体进行抗病分析，发现接种玉米圆斑菌（Cochliobolus carbonum）、玉米大斑菌（Setosphaeria turcica）、玉米小斑菌（Cochliobolus heterostrophus）和禾谷镰孢（F. graminearum）后，zmstp2和zmstp20突变体的病斑面积显著高于野生型B73。本研究在全基因组层面对玉米糖转运蛋白编码基因进行了系统性鉴定和分析，明确了玉米糖转运蛋白的编码基因，揭示了糖转运蛋白编码基因在玉米不同组织及生物和非生物胁迫中的表达规律，为进一步阐明其功能奠定了重要的理论基础。

Abstract:

Sugar is an indispensable source of energy for plant growth and development, and it requires the participation of sugar transporter proteins (STPs) for crossing the hydrophobic barrier in plants. Here, we systematically identified the genes encoding sugar transporters in the genome of maize (Zea mays L.), analyzed their expression patterns under different conditions, and determined their functions in disease resistance. The results showed that the mazie sugar transporter family contained 24 members, all of which were predicted to be distributed on the cell membrane and had a highly conserved transmembrane transport domain. The tissue-specific expression of the maize sugar transporter genes was analyzed, and the expression level of these genes was found to be significantly different in different tissues. The analysis of biotic and abiotic stress data showed that the expression levels of the sugar transporter genes changed significantly under different stress factors. The expression levels of ZmSTP2 and ZmSTP20 continued to increase following Fusarium graminearum infection. By performing disease resistance analysis of zmstp2 and zmstp20 mutants, we found that after inoculation with Cochliobolus carbonum, Setosphaeria turcica, Cochliobolus heterostrophus, and F. graminearum, the lesion area of the mutants was significantly higher than that of the wild-type B73 plant. In this study, the genes encoding sugar transporters in maize were systematically identified and analyzed at the whole genome level. The expression patterns of the sugar transporter-encoding genes in different tissues of maize and under biotic and abiotic stresses were revealed, which laid an important theoretical foundation for further elucidation of their functions.

Key words: maize , sugar transporter , gene expression , disease resistance

MA Yu-xin, ZHOU Zhi-jun, CAO Hong-zhe, ZHOU Fan, SI He-long, ZANG Jin-ping, XING Ji-hong, ZHANG Kang, DONG Jin-gao. 玉米糖转运蛋白家族基因的鉴定和表达分析以及ZmSTP2和ZmSTP20在抗病中的作用[J]. Journal of Integrative Agriculture, 2023, 22(11): 3458-3473.

MA Yu-xin, ZHOU Zhi-jun, CAO Hong-zhe, ZHOU Fan, SI He-long, ZANG Jin-ping, XING Ji-hong, ZHANG Kang, DONG Jin-gao. Identification and expression analysis of sugar transporter family genes reveal the role of ZmSTP2 and ZmSTP20 in maize disease resistance[J]. Journal of Integrative Agriculture, 2023, 22(11): 3458-3473.

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玉米糖转运蛋白家族基因的鉴定和表达分析以及ZmSTP2和ZmSTP20在抗病中的作用

Identification and expression analysis of sugar transporter family genes reveal the role of ZmSTP2 and ZmSTP20 in maize disease resistance

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