敲除草地贪夜蛾的气味受体共受体基因揭示了雌雄虫嗅觉系统的功能差异

doi:10.1016/j.jia.2022.11.004

Journal of Integrative Agriculture ›› 2023, Vol. 22 ›› Issue (7): 2162-2172.DOI: 10.1016/j.jia.2022.11.004

敲除草地贪夜蛾的气味受体共受体基因揭示了雌雄虫嗅觉系统的功能差异

收稿日期:2022-08-08 接受日期:2022-09-27 出版日期:2023-07-20 发布日期:2022-09-27

Mutagenesis of odorant coreceptor Orco reveals the distinct role of olfaction between sexes in Spodoptera frugiperda

CAO Song^{1, 2}, SUN Dong-dong², LIU Yang², YANG Qing¹, WANG Gui-rong^{1, 2#}

¹ Shenzhen Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of
Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences, Shenzhen
518120, P.R.China
² State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of
Agricultural Sciences, Beijing 100193, P.R.China

Received:2022-08-08 Accepted:2022-09-27 Online:2023-07-20 Published:2022-09-27
About author:CAO Song, E-mail: csong_onnz@163.com; #Correspondence WANG Gui-rong, E-mail: wangguirong@caas.cn
Supported by:
This work was funded by the National Natural Science Foundation of China (32130089), the China Postdoctoral Science Foundation (2020M680785), and the Shenzhen Science and Technology Program, China (KQTD20180411143628272), the Central Public-interest Scientific Institution Basal Research Fund of Chinese Academy of Agricultural Sciences (CAAS-ZDRW202108), the Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District, China (PT202101-02), and the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences (ASTIP).

摘要/Abstract

摘要：

昆虫的气味受体（Odorant receprot，OR）是它们探测和识别与其生存密切相关的外部化学线索的关键。昆虫识别配体的特异性受体与一个广泛存在的气味受体共受体（Odorant receptor coreceptor，Orco）构成了异聚体复合体。本研究运用CRISPR/Cas9基因敲除技术对我国重大入侵害虫草地贪夜蛾的Orco基因进行了敲除，并揭示了该基因在气味受体调控的嗅觉行为中的重要作用。触角电位结果表明，Orco基因敲除之后，雌雄成虫对8种测试的植物挥发物和两种性信息素的电生理反应均丧失。然而，Orco基因对雌雄成虫交配行为的影响却存在明显的差异：雄虫Orco突变体的交配行为完全被干扰而雌虫Orco突变体的交配行为却不受影响。产卵实验结果表明，Orco突变体雌虫的产卵量比野生型雌虫的产卵量减少了24.1%。总之，这些结果表明了Orco是一个干扰草地贪夜蛾正常行为的优良靶标，并为害虫的防治提供了一种可行的方法。

Abstract:

Odorant receptor (OR) is crucial for insects to detect and recognize external chemical cues closely related to their survival. The insect OR forms a heteromeric complex composed of a ligand-specific receptor and a ubiquitously odorant receptor coreceptor (Orco). This study used the CRISPR/Cas9 technique to knock out (KO) Orco and reveal its essential role in acting on OR-meditated olfactory behavior in a critical invasive agricultural pest, the fall armyworm (FAW), Spodoptera frugiperda. Electroantennogram (EAG) results suggested that the Orco mutants of both male and female moths severely reduced their electrophysiological responses to the eight tested plant volatiles and two sex pheromones. However, the Orco gene played distinct roles in mating behavior between sexes: the mating behavior was fully disrupted in mutated males but not in mutated females. The oviposition result indicated that the Orco KO females displayed reduced egg laying by 24.1% compared with the mated wild type (WT) females. Overall, these results strongly suggest that Orco is an excellent target for disrupting FAW’s normal behavior and provides a feasible pest control approach.

Key words: Spodoptera frugiperda , Orco , CRISPR/Cas9 , EAG , olfactory behavior

CAO Song, SUN Dong-dong, LIU Yang, YANG Qing, WANG Gui-rong. 敲除草地贪夜蛾的气味受体共受体基因揭示了雌雄虫嗅觉系统的功能差异[J]. Journal of Integrative Agriculture, 2023, 22(7): 2162-2172.

CAO Song, SUN Dong-dong, LIU Yang, YANG Qing, WANG Gui-rong.

Mutagenesis of odorant coreceptor Orco reveals the distinct role of olfaction between sexes in Spodoptera frugiperda [J]. Journal of Integrative Agriculture, 2023, 22(7): 2162-2172.

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敲除草地贪夜蛾的气味受体共受体基因揭示了雌雄虫嗅觉系统的功能差异

Mutagenesis of odorant coreceptor Orco reveals the distinct role of olfaction between sexes in Spodoptera frugiperda

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