谷子EMS突变体叶绿素含量、气孔导度及光合速率变异分析

doi:10.1016/j.jia.2022.10.014

摘要/Abstract

摘要：

叶绿素(Chl)含量，尤其是Chl b含量和气孔导度(G_s)是影响净光合速率(P_n)的关键因素。谷子(Setaria italica)是二倍体C₄禾本科植物，基因组简单，转化效率高，已被广泛应用于光合作用和耐旱研究中。本研究对48株EMS诱变的谷子材料进行了Chl含量、G_s以及P_n的表征。发现分别有24、34以及35个突变体材料在Chl含量、G_s以及P_n上发生了显著的变异。相关分析表明，G_s的增加与P_n的增加存在显著正相关关系，Chl b含量的降低与Pn的降低存在弱相关关系。值得注意的是，我们鉴定到两个突变体，Chl b含量显著低于对照豫谷1号，但P_n显著高于豫谷1号。同时，有7个突变体，G_s显著降低于对照，但P_n不显著降低。由此，本研究鉴定了丰富的遗传变异株系，对这些株系对进一步研究将有助于解析Chl含量、G_s和P_n之间的关系，以及C₄物种高效光合和节水的机理

Abstract:

Chlorophyll (Chl) content, especially Chl b content, and stomatal conductance (G_s) are key factors that greatly affect net photosynthetic rate (P_n). Setaria italica, a diploid C₄ panicoid species with a simple genome and high transformation efficiency, has been widely accepted as a model in photosynthesis and drought-tolerance research. In the current study, Chl content, G_s, and P_n of 48 Setaria mutants induced by ethyl methanesulfonate were characterized. A total of 24, 34 and 35 mutants had significant variations in Chl content, G_s, and P_n, respectively. Correlation analysis showed that positive correlation exists between increased G_s and increased Pn, and a weak correlation between decreased Chl b content and decreased P_n was also found. Remarkably, two mutants behaved significantly decreased Chl b content but increased P_n when compared that of Yugu 1. Seven mutants behaved significantly decreased G_s but non-decreasing P_n when compared that of Yugu 1. The current study thus identified various genetic lines, further exploration of which would be beneficial to elucidate the relationship between Chl content, G_s and P_n and the mechanism underlying why C₄ species are efficient at photosynthesis and water saving.

Key words: photosynthetic capacity , chlorophyll content , stomatal conductance , EMS mutant variation , Setaria italica

TANG Chan-juan, LUO Ming-zhao, ZHANG Shuo, JIA Guan-qing, TANG Sha, JIA Yan-chao, ZHI Hui, DIAO Xian-min. 谷子EMS突变体叶绿素含量、气孔导度及光合速率变异分析[J]. Journal of Integrative Agriculture, 2023, 22(6): 1618-1630.

TANG Chan-juan, LUO Ming-zhao, ZHANG Shuo, JIA Guan-qing, TANG Sha, JIA Yan-chao, ZHI Hui, DIAO Xian-min. Variations in chlorophyll content, stomatal conductance and photosynthesis in Setaria EMS mutants[J]. Journal of Integrative Agriculture, 2023, 22(6): 1618-1630.

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